Mouse ALCAM/CD166 Biotinylated Antibody Summary
Accession # AAC06342
Perfusion fixed frozen sections of mouse liver
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
ALCAM/CD166 in Mouse Liver. ALCAM/CD166 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse ALCAM/CD166 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1172) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation (1‑4).
- Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
- Aruffo, A. et al. (1997) Immunol. Today 18:498.
- Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
- Van Kempen, L. et al. (2001) J. Biol. Chem. 276:25783.
Citations for Mouse ALCAM/CD166 Biotinylated Antibody
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Isolation and characterization of endosteal niche cell populations that regulate hematopoietic stem cells.
Authors: Nakamura Y, Arai F, Iwasaki H
Sample Types: Whole Cells
Applications: Flow Cytometry
Combination of aging and dimethylhydrazine treatment causes an increase in cancer-stem cell population of rat colonic crypts.
Authors: Levi E, Misra S, Du J, Patel BB, Majumdar AP
Sample Types: Whole Tissue
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