Human/Mouse/Rat/Canine ALCAM/CD166 Antibody
Human/Mouse/Rat/Canine ALCAM/CD166 Antibody Summary
Trp28-Lys527
Accession # AAC06342
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat ALCAM/CD166 by Western Blot. Western blot shows lysates of mouse brain (cerebellumn) tissue, rat brain (cerebellum) tissue, H4-II-E-C3 rat hepatoma cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ALCAM/CD166 at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of ALCAM/CD166 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) activated or (B) resting were stained with Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C).
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Detection of ALCAM/CD166 in Canine PBMCs by Flow Cytometry. Canine peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
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ALCAM/CD166 in Mouse Liver. ALCAM/CD166 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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ALCAM/CD166 in Rat Mesenchymal Stem Cells. ALCAM/CD166 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Mouse ALCAM/CD166 by Simple WesternTM. Simple Western lane view shows lysates of mouse brain tissue and mouse lung tissue loaded at 0.2 mg/mL. A specific band was detected for ALCAM/CD166 at approximately 149 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ALCAM/CD166
ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain, and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation (1-4). The ECD of mouse ALCAM shares 97.5% and 93.2% aa sequence identity with rat and canine ALCAM ECD, respectively.
- Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
- Aruffo, A. et al. (1997) Immunol. Today 18:498.
- Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
- Van Kempen, L. et al. (2001) J. Biol. Chem. 276:25783.
Product Datasheets
Citations for Human/Mouse/Rat/Canine ALCAM/CD166 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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CD166-specific CAR-T cells potently target colorectal cancer cells
Authors: S He, S Li, J Guo, X Zeng, D Liang, Y Zhu, Y Li, D Yang, X Zhao
Translational Oncology, 2022;27(0):101575.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A comprehensive library of human transcription factors for cell fate engineering
Authors: AHM Ng, P Khoshakhla, JE Rojo Arias, G Pasquini, K Wang, A Swiersy, SL Shipman, E Appleton, K Kiaee, RE Kohman, A Vernet, M Dysart, K Leeper, W Saylor, JY Huang, A Graveline, J Taipale, DE Hill, M Vidal, JM Melero-Mar, V Busskamp, GM Church
Nature Biotechnology, 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
Authors: HF Renard, F Tyckaert, C Lo Giudice, T Hirsch, CA Valades-Cr, C Lemaigre, M Shafaq-Zad, C Wunder, R Wattiez, L Johannes, P van der Br, D Alsteens, P Morsomme
Nat Commun, 2020;11(1):1457.
Species: Mouse
Sample Types: Whole Cells
Applications: IF -
Axonal growth of midbrain dopamine neurons is modulated by the cell adhesion molecule ALCAM through trans-heterophilic interaction with L1Cam, ChL1 and Semaphorin
Authors: CR Bye, V Rytova, WF Alsanie, CL Parish, LH Thompson
J. Neurosci., 2019;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2018;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Tbr1 instructs laminar patterning of retinal ganglion cell dendrites
Authors: J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes
Nat. Neurosci., 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dual role of ALCAM in neuroinflammation and blood-brain barrier homeostasis
Authors: MA L‚cuyer, O Saint-Laur, L BourbonniŠ, S Larouche, C Larochelle, L Michel, M Charabati, M Abadier, S Zandee, N Haghayegh, E Gowing, C Pittet, R Lyck, B Engelhardt, A Prat
Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E524-E533.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.
Authors: Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L
Proc Natl Acad Sci U S A, 2015;112(15):E1946-55.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
Authors: Gu, Zirong, Imai, Fumiyasu, Kim, In Jung, Fujita, Hiroko, Katayama, Kei ichi, Mori, Kensaku, Yoshihara, Yoshihir, Yoshida, Yutaka
PLoS ONE, 2015;10(3):e0121550.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dbx1 triggers crucial molecular programs required for midline crossing by midbrain commissural axons.
Authors: Inamata, Yasuyuki, Shirasaki, Ryuichi
Development, 2014;141(6):1260-71.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Ionizing irradiation induces acute haematopoietic syndrome and gastrointestinal syndrome independently in mice.
Authors: Leibowitz B, Wei L, Zhang L, Ping X, Epperly M, Greenberger J, Cheng T, Yu J
Nat Commun, 2014;5(0):3494.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cocaine hijacks sigma1 receptor to initiate induction of activated leukocyte cell adhesion molecule: implication for increased monocyte adhesion and migration in the CNS.
Authors: Yao H, Kim K, Duan M, Hayashi T, Guo M, Morgello S, Prat A, Wang J, Su TP, Buch S
J. Neurosci., 2011;31(16):5942-55.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Molecular mechanisms controlling midline crossing by precerebellar neurons.
Authors: Di Meglio T, Nguyen-Ba-Charvet KT, Tessier-Lavigne M, Sotelo C, Chedotal A
J. Neurosci., 2008;28(25):6285-94.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Inhibition of p38 MAPK signaling in chondrocyte cultures results in enhanced osteogenic differentiation of perichondral cells.
Authors: Stanton LA, Beier F
Exp. Cell Res., 2007;313(1):146-55.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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