Mouse Alkaline Phosphatase/ALPL Antibody

Catalog # Availability Size / Price Qty
AF2910
AF2910-SP

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Detection of Mouse Alkaline Phosphatase/ALPL by Western Blot.
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Product Details
Citations (22)
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Reviews (2)

Mouse Alkaline Phosphatase/ALPL Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Alkaline Phosphatase/ALPL in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Alkaline Phosphatase/ALPL
Phe18-Gly503
Accession # P09242
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Alkaline Phosphatase/ALPL (Catalog # 2910-AP), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse Alkaline Phosphatase/ALPL antibody by Western Blot. View Larger

Detection of Mouse Alkaline Phosphatase/ALPL by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of ALPL antibody in Rat Stem Cells antibody by Flow Cytometry. View Larger

Detection of ALPL in Rat Stem Cells by Flow Cytometry. Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunohistochemistry Alkaline Phosphatase/ALPL antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Alkaline Phosphatase/ALPL in Mouse Embryo. Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2910) at 2 µg/mL overnight at 4 °C. Tissue was stained red and counterstained with DAPI (blue).Image courtesy of Paul J. Simmons, Ph.D., Director, Center for Stem Cell Research, The University of Texas Health Science Center at Houston, Houston, Texas, USA.View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunocytochemistry Alkaline Phosphatase/ALPL antibody in Rat Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

Alkaline Phosphatase/ALPL in Rat Mesenchymal Stem Cells. Alkaline Phosphatase/ALPL was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Alkaline Phosphatase/ALPL

Several distinct genes encode alkaline phosphatases (APs) in mice with different tissue-specific expression patterns. The Alpl gene, also known as Akp2, encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP) (1). The Alpl gene is a key regulator of bone mineralization in mice (2). A variety of mutations in the human ALPL gene leads to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 504 to 524) is not present in the mature form.

References
  1. Terao, M. and B. Mintz (1987) Proc. Natl. Acad. Sci. USA 84:7051.
  2. Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9445.
  3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Long Name
Alkaline Phosphatase Liver
Entrez Gene IDs
249 (Human); 11647 (Mouse)
Alternate Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

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Citations for Mouse Alkaline Phosphatase/ALPL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Orchestration of energy metabolism and osteogenesis by Mg2+ facilitates low-dose BMP-2-driven regeneration
    Authors: S Lin, S Yin, J Shi, G Yang, X Wen, W Zhang, M Zhou, X Jiang
    Bioactive materials, 2022;18(0):116-127.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Single-cell RNA landscape of the osteoimmunology microenvironment in periodontitis
    Authors: Y Chen, H Wang, Q Yang, W Zhao, Y Chen, Q Ni, W Li, J Shi, W Zhang, L Li, Y Xu, H Zhang, D Miao, L Xing, W Sun
    Theranostics, 2022;12(3):1074-1096.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. The Non-Erythropoietic EPO Analogue Cibinetide Inhibits Osteoclastogenesis In Vitro and Increases Bone Mineral Density in Mice
    Authors: Z Awida, A Bachar, H Saed, A Gorodov, N Ben-Califa, M Ibrahim, A Kolomansky, JA Iden, L Graniewitz, T Liron, S Hiram-Bab, M Brines, Y Gabet, D Neumann
    International Journal of Molecular Sciences, 2021;23(1):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The m6A demethylase FTO promotes the osteogenesis of mesenchymal stem cells by downregulating PPARG
    Authors: LS Chen, M Zhang, P Chen, XF Xiong, PQ Liu, HB Wang, JJ Wang, J Shen
    Acta pharmacologica Sinica, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Mitochondrial TNAP controls thermogenesis by hydrolysis of phosphocreatine
    Authors: Y Sun, JF Rahbani, MP Jedrychows, CL Riley, S Vidoni, D Bogoslavsk, B Hu, PA Dumesic, X Zeng, AB Wang, NH Knudsen, CR Kim, A Marasciull, JL Millán, ET Chouchani, L Kazak, BM Spiegelman
    Nature, 2021;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation
    Authors: S Yu, J Guo, Z Sun, C Lin, H Tao, Q Zhang, Y Cui, H Zuo, Y Lin, S Chen, H Liu, Z Chen
    Cell Death & Disease, 2021;12(2):197.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. T cell-depleting nanoparticles ameliorate bone loss by reducing activated T cells and regulating the Treg/Th17 balance
    Authors: X Yang, F Zhou, P Yuan, G Dou, X Liu, S Liu, X Wang, R Jin, Y Dong, J Zhou, Y Lv, Z Deng, S Liu, X Chen, Y Han, Y Jin
    Bioactive materials, 2021;6(10):3150-3163.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Impaired autophagy triggered by HDAC9 in mesenchymal stem cells accelerates bone mass loss
    Authors: L Zhang, M Qi, J Chen, J Zhao, L Li, J Hu, Y Jin, W Liu
    Stem Cell Res Ther, 2020;11(1):269.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. 4-phenylbutyric acid enhances the mineralization of osteogenesis imperfecta iPSC-derived osteoblasts
    Authors: S Takeyari, T Kubota, Y Ohata, M Fujiwara, T Kitaoka, Y Taga, K Mizuno, K Ozono
    J Biol Chem, 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. A polysaccharide from the dried rhizome of Drynaria fortunei (Kunze) J. Sm. prevents ovariectomized (OVX)-induced osteoporosis in rats
    Authors: X Sun, B Wei, Z Peng, X Chen, Q Fu, C Wang, J Zhen, J Sun
    J. Cell. Mol. Med., 2020;0(0):.
    Species: Rat
    Sample Types: Serum
  11. The Human Amniotic Mesenchymal Stem Cells (hAMSCs) Improve the Implant Osseointegration and Bone Regeneration in Maxillary Sinus Floor Elevation in Rabbits
    Authors: L Yin, ZX Zhou, M Shen, N Chen, F Jiang, SL Wang
    Stem Cells Int, 2019;2019(0):9845497.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers
    Authors: T Kazi, I Niibe, A Nishikawa, T Matsuzaki
    FEBS Open Bio, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  13. Responses of primary osteoblasts and osteoclasts from hemizygous beta-globin knockout thalassemic mice with elevated plasma glucose to 1,25-dihydroxyvitamin D3
    Authors: N Charoenpha, R Aeimlapa, S Sooksawanw, J Thongbunch, J Teerapornp, S Svasti, K Wongdee
    Sci Rep, 2019;9(1):13963.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. Morphological and functional analyses of skeletal muscles from an immunodeficient animal model of limb girdle muscular dystrophy type 2E
    Authors: G Giovannell, G Giacomazzi, H Grosemans, M Sampaolesi
    Muscle Nerve, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration
    Authors: MJ Carr, JS Toma, APW Johnston, PE Steadman, SA Yuzwa, N Mahmud, PW Frankland, DR Kaplan, FD Miller
    Cell Stem Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3? Complex in Hypophosphatasia
    Authors: W Liu, L Zhang, K Xuan, C Hu, L Li, Y Zhang, F Jin, Y Jin
    Theranostics, 2018;8(20):5575-5592.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Tissue-Nonspecific Alkaline Phosphatase Is Required for MC3T3 Osteoblast-Mediated Protection of Acute Myeloid Leukemia Cells from Apoptosis
    Authors: RM Sterner, KN Kremer, A Dudakovic, JJ Westendorf, AJ van Wijnen, KE Hedin
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Cardiac Fibroblasts Adopt Osteogenic Fates and Can Be Targeted to Attenuate Pathological Heart Calcification
    Authors: Arjun Deb
    Cell Stem Cell, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair.
    Authors: Yoshizaki, Keigo, Hu, Lizhi, Nguyen, Thai, Sakai, Kiyoshi, He, Bing, Fong, Chak, Yamada, Yoshihik, Bikle, Daniel D, Oda, Yuko
    PLoS ONE, 2014;9(6):e99991.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. DLX3 regulates bone mass by targeting genes supporting osteoblast differentiation and mineral homeostasis in vivo.
    Authors: Isaac, J, Erthal, J, Gordon, J, Duverger, O, Sun, H-W, Lichtler, A C, Stein, G S, Lian, J B, Morasso, M I
    Cell Death Differ, 2014;21(9):1365-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Midkine-deficiency increases the anabolic response of cortical bone to mechanical loading.
    Authors: Liedert A, Mattausch L, Rontgen V, Blakytny R, Vogele D, Pahl M, Bindl R, Neunaber C, Schinke T, Harroch S, Amling M, Ignatius A
    Bone, 2011;48(4):945-51.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Mouse Alkaline Phosphatase/ALPL Antibody

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Mouse Alkaline Phosphatase/ALPL Antibody
By Anonymous on 04/29/2021
Application: ELISA Sample Tested: Bone marrow cells Species: Mouse

Mouse bone marrow cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody or followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.


Mouse Alkaline Phosphatase/ALPL Antibody
By Anonymous on 06/28/2016
Application: Western Blot Sample Tested: Differentiated osteoblasts Species: Mouse