Mouse Aminopeptidase A/ENPEP Antibody

Catalog # Availability Size / Price Qty
AF2809
AF2809-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Mouse Aminopeptidase A/ENPEP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Aminopeptidase A/ENPEP in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human ENPEP is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase A/ENPEP
Arg41-Pro945
Accession # P16406
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL

Recombinant Mouse Aminopeptidase A/ENPEP

Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Aminopeptidase A/ENPEP, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase A/ENPEP

The mouse ENPEP gene encodes Aminopeptidase A (APA), which is also known as glutamyl aminopeptidase (EAP), aspartate aminopeptidase, angiotensinase A, Ca2+-activated glutamate aminopeptidase, membrane aminopeptidase II and the BP-1/6C3 antigen (2). The various names reflect its substrate specificity (prefer Glu or Asp and angiotensin II as one of the physiological substrates), Ca2+-dependence, cellular location (type II membrane protein) and immunological reactivity to antibody BP-1/6C3. The deduced amino acid sequence of mouse ENPEP consists of a short cytoplasmic tail (residues 1 to 18), a transmembrane region (residue 19 to 39), and a long ectodomain (residues 40 to 945) (1). In addition to the N-terminal zinc metalloprotease domain, the ectodomain also contains the C‑terminal region, which functions as an intramolecular chaperone required for the correct folding, cell surface expression and activity (3). The purified rmENPEP consists of the ectodomain and is active in the assay described above.

References
  1. Wu, Q. et al. (1990) Proc. Natl. Acad. Sci. USA 87:993.
  2. Wang, J. and M.D. Cooper (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 299, Academic Press, San Diego.
  3. Rozenfeld, R. et al. (2004) J. Biol. Chem. 279:43285.
Entrez Gene IDs
2028 (Human); 13809 (Mouse)
Alternate Names
Aminopeptidase A; APA; AP-A; CD249 antigen; CD249; Differentiation antigen gp160; EAP; EC 3.4.11; EC 3.4.11.7; ENPEP; glutamyl aminopeptidase (aminopeptidase A); glutamyl aminopeptidase; gp160

Product Datasheets

You must select a language.

x

Citation for Mouse Aminopeptidase A/ENPEP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Quantitative proteomics of MDCK cells identify unrecognized roles of clathrin adaptor AP-1 in polarized distribution of surface proteins
    Authors: PS Caceres, D Gravotta, PJ Zager, N Dephoure, E Rodriguez-
    Proc. Natl. Acad. Sci. U.S.A., 2019;116(24):11796-11805.
    Species: Canine
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse Aminopeptidase A/ENPEP Antibody

There are currently no reviews for this product. Be the first to review Mouse Aminopeptidase A/ENPEP Antibody and earn rewards!

Have you used Mouse Aminopeptidase A/ENPEP Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review