Mouse ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody

Catalog #: AF4886 Datasheet / COA / SDS

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AF4886
AF4886-SP

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Detection of Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL by Western Blot.

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Mouse ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) ASAHL is observed and less than 5% cross-reactivity rhASAHL-2 and recombinant mouse ASAHL-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ASAHL/N‑acylethanolamine-hydrolyzing Acid A
Val33-Ser362
Accession # AAH04572

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4886-AH), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4886) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase

The mouse ASAHL gene encodes N-acylethanolamine-hydrolyzing Acid Amidase (NAAA), a fatty acid amidase with maximal activity at acidic pH (1). NAAA hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl-, N-oleoyl-, and N-arachidonoyl, but is most active against N-palmitoylethanolamine (2). NAAA is a member of the choloylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (1). NAAA is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (1). Through its amidase activity, ASAHL may play a role in the termination of the actions of a variety of N-acylethanolamides (3). NAAA can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonoyl fluorophosphonate (2).

References

Tsuboi, K. et al. (2005) J. Biol. Chem. 280:11082.
Ueda, N. et al. (2001) J. Biol. Chem. 276:35552.
Sun, Y. X. et al. (2005) Biochim. Biophys. Acta 1736:211.

Entrez Gene IDs

27163 (Human); 67111 (Mouse); 497009 (Rat)

Alternate Names

Acid ceramidase-like protein; ASAHL; ASAH-like protein; EC 3.5.1.-; NAAA; N-acylethanolamine acid amidase; Nacylethanolaminehydrolyzing Acid Amidase; N-acylethanolamine-hydrolyzing acid amidase; N-acylsphingosine amidohydrolase (acid ceramidase)-like; N-acylsphingosine amidohydrolase-like; PLT