Detects mouse ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) ASAHL is observed and less than 5% cross-reactivity rhASAHL-2 and recombinant mouse ASAHL-2 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant mouse ASAHL/N‑acylethanolamine-hydrolyzing Acid A Val33-Ser362 Accession # AAH04572
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4886) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The mouse ASAHL gene encodes N-acylethanolamine-hydrolyzing Acid Amidase (NAAA), a fatty acid amidase with maximal activity at acidic pH (1). NAAA hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl-, N-oleoyl-, and N-arachidonoyl, but is most active against N-palmitoylethanolamine (2). NAAA is a member of the choloylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (1). NAAA is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (1). Through its amidase activity, ASAHL may play a role in the termination of the actions of a variety of N-acylethanolamides (3). NAAA can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonoyl fluorophosphonate (2).
Tsuboi, K. et al. (2005) J. Biol. Chem. 280:11082.
Ueda, N. et al. (2001) J. Biol. Chem. 276:35552.
Sun, Y. X. et al. (2005) Biochim. Biophys. Acta 1736:211.
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