Detection of Mouse Bag-1 by Western Blot.
Western blot shows lysates of CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.6 µg/mL of Goat Anti-Mouse Bag-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF815) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bag-1 at approximately 32 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Immunoprecipitation of Mouse Bag‑1.
Bag‑1 was immunoprecipitated from lysates (3 x 106 cells) of CTLL‑2 mouse cytotoxic T cell line and A431 human epithelial carcinoma cell line following incubation with 5 µg Goat Anti-Mouse Bag‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF815) for 1 hour at 4 ºC. Bag‑1-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated Bag‑1 was detected by Western blot using 5 µg/mL Mouse Bag‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF815). The leftmost lane represents a control experiment where the antibody was incubated with no cell lysate. View our recommended buffer recipes for immunoprecipitation.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Bag-1 (Bcl-2-associated Athanogene 1) is a 31-kDa protein with an apparent molecular mass of 46 kDa that can bind to Bcl-2 and enhance its anti-apoptotic effect, resulting in increased protection from cell death induced by apoptotic stimuli. Bag-1 shares no significant homology with Bcl-2 or other Bcl-2 family proteins. The C-terminal region of Bag-1 binds the PDGF and HGF Receptor, and enhances growth factor-mediated protection from apoptosis. This represents a link between growth factor receptors and anti-apoptotic mechanisms.
Entrez Gene IDs:
573 (Human); 12017 (Mouse)
BAG family molecular chaperone regulator 1; Bag1; Bag-1; Bcl-2 associating athanogene-1 protein; Bcl-2-associated athanogene 1; BCL2-associated athanogene; Bcl-2-binding protein; glucocortoid receptor-associated protein RAP46,46-KD; HAP; RAP46
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