Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Hamster - Cricetulus (Chinese Hamster), Transgenic Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Bag-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Bag-1

Specificity

Detects mouse Bag-1 in Western blots. Does not react with human Bag-1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Bag‑1 Antibody

Detection of Mouse Bag-1 antibody by Western Blot.

Detection of Mouse Bag-1 by Western Blot.

Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.6 µg/mL of Goat Anti-Mouse Bag-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF815) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bag-1 at approximately 32 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Immunoprecipitation of Mouse Bag‑1.

Immunoprecipitation of Mouse Bag‑1.

Bag‑1 was immunoprecipitated from lysates (3 x 106cells) of CTLL‑2 mouse cytotoxic T cell line and A431 human epithelial carcinoma cell line following incubation with 5 µg Goat Anti-Mouse Bag‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF815) for 1 hour at 4 ºC. Bag‑1-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated Bag‑1 was detected by Western blot using 5 µg/mL Mouse Bag‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF815). The leftmost lane represents a control experiment where the antibody was incubated with no cell lysate. View our recommended buffer recipes for immunoprecipitation.

Applications for Mouse Bag‑1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 µg/106 cells
Sample: CTLL‑2 mouse cytotoxic T cell line, see our available Western blot detection antibodies

Western Blot

5 µg/mL
Sample: Bag‑1 immunoprecipitated from lysates of CTLL‑2 mouse cytotoxic T cell line and A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bag-1

Bag-1 (Bcl-2-associated Athanogene 1) is a 31-kDa protein with an apparent molecular mass of 46 kDa that can bind to Bcl-2 and enhance its anti-apoptotic effect, resulting in increased protection from cell death induced by apoptotic stimuli. Bag-1 shares no significant homology with Bcl-2 or other Bcl-2 family proteins. The C-terminal region of Bag-1 binds the PDGF and HGF Receptor, and enhances growth factor-mediated protection from apoptosis. This represents a link between growth factor receptors and anti-apoptotic mechanisms.

Long Name

Bcl-2-associated Anthanogene-1

Alternate Names

Bag1

Entrez Gene IDs

573 (Human); 12017 (Mouse)

Gene Symbol

BAG1

Additional Bag-1 Products

Product Documents for Mouse Bag‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Bag‑1 Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Mouse Bag‑1 Antibody

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