Mouse Cadherin-17 Antibody Summary
Accession # Q9R100
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Cadherin‑17 by Western Blot. Western blot shows lysates of mouse colon tissue and mouse spleen tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Mouse Cadherin‑17 Monoclonal Antibody (Catalog # MAB8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cadherin‑17 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cadherin‑17 in Mouse Intestine. Cadherin‑17 was detected in perfusion fixed frozen sections of mouse intestine using Rabbit Anti-Mouse Cadherin‑17 Monoclonal Antibody (Catalog # MAB8524) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Mouse Cadherin‑17 by Simple WesternTM. Simple Western lane view shows lysates of mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin‑17 at approximately 153 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Mouse Cadherin‑17 Monoclonal Antibody (Catalog # MAB8524). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. It associates homotypically and is selectively expressed on gastric and intestinal mucosa.
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Cell and Tissue Staining Kits
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