Mouse Cadherin-17 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8524
MAB8524-SP
Detection  of Mouse Cadherin‑17 by Western Blot.
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Mouse Cadherin-17 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cadherin-17 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1262C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cadherin-17
Glu22-Met786
Accession # Q9R100
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunohistochemistry
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Mouse Cadherin‑17 by Western Blot. View Larger

Detection of Mouse Cadherin‑17 by Western Blot. Western blot shows lysates of mouse colon tissue and mouse spleen tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Mouse Cadherin‑17 Monoclonal Antibody (Catalog # MAB8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cadherin‑17 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cadherin‑17  in Mouse Intestine. View Larger

Cadherin‑17 in Mouse Intestine. Cadherin‑17 was detected in perfusion fixed frozen sections of mouse intestine using Rabbit Anti-Mouse Cadherin‑17 Monoclonal Antibody (Catalog # MAB8524) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western Detection  of Mouse Cadherin‑17 by Simple WesternTM. View Larger

Detection of Mouse Cadherin‑17 by Simple WesternTM. Simple Western lane view shows lysates of mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin‑17 at approximately 153 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Mouse Cadherin‑17 Monoclonal Antibody (Catalog # MAB8524). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-17

Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. It associates homotypically and is selectively expressed on gastric and intestinal mucosa.

Entrez Gene IDs
1015 (Human); 12557 (Mouse)
Alternate Names
cadherin 17, LI cadherin (liver-intestine); cadherin; cadherin-16; Cadherin17; Cadherin-17; CDH16; CDH17; FLJ26931; HPT-1 cadherin; HPT1; HPT-1; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1; Intestinal peptide-associated transporter HPT-1; LI cadherin; LI-cadherin; Liver-intestine cadherin; MGC138218; MGC142024

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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