Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1055C
View all CCR8 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with mouse CCR8
Accession # P56484

Specificity

Stains mouse CCR8 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse CCR8 Antibody

Detection of CCR8 antibody in HEK293 Human Cell Line Transfected with Mouse CCR8 and eGFP antibody by Flow Cytometry.

Detection of CCR8 in HEK293 Human Cell Line Transfected with Mouse CCR8 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse CCR8 and eGFP was stained with either (A) Rabbit Anti-Mouse CCR8 Monoclonal Antibody (Catalog # MAB8324) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).

Applications for Mouse CCR8 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CCR8 and eGFP

Reviewed Applications

Read 1 review rated 3 using MAB8324 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR8

CCR8 (C-C chemokine Receptor 8; also known as CD198) is a 41-43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Mouse CCR8 is a 7-transmembrane protein that is 353 amino acids (aa) in length. It contains a 33 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. In mouse, CCR8 is N- and possibly O-glycosylated, and known to be sulfated on Tyr14 and 15. The unusual nature of these posttranslational modifications may lead to anomalous migration in SDS-PAGE. Over aa sequences 1-33 and 92-105 collectively, mouse CCR8 shares 64% and 85% aa identity with human and rat CCR8, respectively.

Alternate Names

CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1

Entrez Gene IDs

1237 (Human); 12776 (Mouse)

Gene Symbol

CCR8

UniProt

P56484

Additional CCR8 Products

Product Documents for Mouse CCR8 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCR8 Antibody

For research use only

Customer Reviews for Mouse CCR8 Antibody (1)

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  • Mouse CCR8 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: mouse bronchoalveolar lavage, Mouse splenocytes and CD4
    Species: Mouse
    Verified Customer | Posted 08/19/2016
    3-step staining; 1. unconjugated CCR8 antibody 2. Biotinylated anti-rabbit IgG 3. Streptavidin-APC
    Mouse CCR8 Antibody MAB8324

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