Detection of CCR8 in HEK293 Human Cell Line Transfected with Mouse CCR8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse CCR8 and eGFP was stained with either (A) Rabbit Anti-Mouse CCR8 Monoclonal Antibody (Catalog # MAB8324) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
12 months from date of receipt, -20 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 °C under sterile conditions after opening.
CCR8 (C-C chemokine Receptor 8; also known as CD198) is a 41-43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Mouse CCR8 is a 7-transmembrane protein that is 353 amino acids (aa) in length. It contains a 33 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. In mouse, CCR8 is N- and possibly O-glycosylated, and known to be sulfated on Tyr14 and 15. The unusual nature of these posttranslational modifications may lead to anomalous migration in SDS-PAGE. There are two potential isoforms, one that shows a deletion of aa 103-163, and another that shows a Met substitution for aa 125-166. Over aa sequences 1-33 and 92-105 collectively, mouse CCR8 shares 64% and 85% aa identity with human and rat CCR8, respectively.
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