Key Product Details

Species Reactivity

Mouse

Applications

Multiplex Immunofluorescence, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1250D
View all CD11b/Integrin alpha M Primary Antibodies »

Product Specifications

Immunogen

MouseCD11b/Integrin alpha M synthetic peptide
Accession # P11215

Specificity

Detects mouse CD11b/Integrin alpha M in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse CD11b/Integrin alpha M Antibody

Detection of CD11b in Mouse Spleen via seqIF™ staining on COMET™​

CD11b was detected in immersion fixed paraffin-embedded sections of mouse Spleen using Rabbit Anti-Mouse CD11b, Monoclonal Antibody (Catalog #MAB11242) at 3ug/mL dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of Mouse CD11b/Integrin alpha M by Western Blot.

Western blot shows lysates of mouse brain. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11242) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD11b/Integrin alpha M at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Mouse and Rat CD11b/Integrin alpha M by Simple WesternTM.

Simple Western lane view shows lysates of mouse brain and rat brain, loaded at 0.2 mg/mL. A specific band was detected for CD11b/Integrin alpha M at approximately 180-200 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11242). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse CD11b/Integrin alpha M Antibody

Application
Recommended Usage

Multiplex Immunofluorescence

3 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen

Simple Western

5 µg/mL
Sample: Mouse brain and rat brain

Western Blot

0.5 µg/mL
Sample: Mouse brain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD11b/Integrin alpha M

The Integrin alpha M subunit (CD11b), associates with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimeric Integrin CD11b/CD18, also known as Mac-1 and CR-3. Upon activation, CD11b/CD18 is expressed on granulocytes, monocytes, a subset of NK cells and activated lymphocytes. Integrin CD11b/CD18 functions as a receptor for complement fragment iC3b, ICAM-1 (CD54), ICAM-2 (CD102) and fibrinogen to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles (1‑3).

References

  1. Springer, T.A. et al. (1978) Eur. J. Immunol. 8:539.
  2. Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
  3. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.

Alternate Names

CD11b, Integrin alpha M, ITGAM

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Gene Symbol

ITGAM

UniProt

P11215 (Rat)

Additional CD11b/Integrin alpha M Products

Product Documents for Mouse CD11b/Integrin alpha M Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD11b/Integrin alpha M Antibody

For research use only

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