Mouse CD200 R1 Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD200 R1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human CD200 R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD200 R1
    Thr26-Pro238
    Accession # BAE42266
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CD200 R1 Fc Chimera (Catalog # 2554-CD)
  • Flow Cytometry
    2.5 µg/106 cells
    MC/9‑2 mouse masT cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD200 R1 in Mouse Splenocytes. CD200 R1 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD200 R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2554) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD200 R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1‑3). The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, (5‑7) while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). The CD200 R-like molecules may interact differently with CD200 (11, 12). The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals (13‑15). CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 16).

  • References:
    1. Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
    2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483. 
    3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285. 
    4. Wright, G.J. et al. (2003) J. Immunol. 171:3034. 
    5. Shiratori, I. et al. (2005) J. Immunol. 175:4441. 
    6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348. 
    7. Fallarino, F. et al. (2004) J. Immunol. 173:3748. 
    8. Wright, G.J. et al. (2001) Immunology 102:173. 
    9. Hoek, R.M. et al. (2000) Science 290:1768.
    10. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
    11. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
    12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
    13. Zhang, S. and J.H. Phillips (2006) J. Leukoc. Biol. 79:363.
    14. Zhang, S. et al. (2004) J. Immunol. 173:6786.
    15. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
    16. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.
  • Long Name:
    CD200 Receptor 1
  • Entrez Gene IDs:
    131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)
  • Alternate Names:
    CD200 R1; CD200 receptor 1; CD200R1; CD200RMOX2Rcell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; CRTR2; HCRTR2; MOX2 receptor; MOX2R; OX2RCD200 cell surface glycoprotein receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. CD200R/Foxp3-mediated signalling regulates microglial activation.
    Authors: Yi M, Zhang E, Kim J, Baek H, Shin N, Kim S, Kim S, Kim H, Lee S, Park J, Kim Y, Kwon O, Lee Y, Oh S, Kim D
    Sci Rep, 2016;6(0):34901.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. The immunosuppressive surface ligand CD200 augments the metastatic capacity of squamous cell carcinoma.
    Authors: Stumpfova M, Ratner D, Desciak EB, Eliezri YD, Owens DM
    Cancer Res., 2010;70(7):2962-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
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