Mouse CD97 isoform 1 Antibody

Catalog # Availability Size / Price Qty
Detection of CD97 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (2)
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Mouse CD97 isoform 1 Antibody Summary

Species Reactivity
Detects mouse CD97 isoform 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD97 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant mouse CD97 isoform 1
Accession # Q9Z0M6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse CD97 Variant 2 (Catalog # 5494-CD)
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD97 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD97 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse CD97 isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3734, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD97

CD97 is a member of the LNB-TM7 protein family, which is a subfamily of the G-protein coupled receptor 2 family (1‑3). Molecules in this family have EGF-like modules coupled to class B G protein 7-transmembrane (TM) domains by a glycosylated (mucin) stalk. Three isoforms of CD97 are produced by alternative splicing in mouse. Isoform 1, which is the longest, contains four EGF-like domains. Only the N-terminal EGF-like domain does not bind calcium. In isoform 2, which is described in this insert, does not contain this sequence that corresponds to amino acid (aa) 120‑213 in isoform 1. Compared to isoform 1, isoform 3 lacks a 45 aa sequence between EGF-like domains 2 and 3 (4, 5). Cells known to express CD97 include monocytes, macrophages, T cells, select B cells, dendritic cells and, potentially, vascular and visceral smooth muscle cells (1, 6‑7). CD97 is also differentially expressed on murine hematopoietic stem- and progenitor-cells (7). CD55 (decay accelerating factor), a GPI-linked cell surface molecule with short consensus repeats that regulates complement activation on cell surfaces, chondroiten sulfate and the integrin alpha 5 beta (also known as VLA-5) have been identified as cellular ligands for CD97 (7). The composition of the EGF domain region defines the ligand specificity of the different CD97 isoforms (7). The first and second EGF domains interact with CD55, whereas the fourth EGF domain binds chondroitin sulfate (7). The ligand affinity of the CD97 isoforms differs (7). While affinity for CD55 is significantly higher for the smaller isoforms, chondroitin sulfate interacts exclusively with the largest isoforms (7). It has been demonstrated that CD97 is required for neutrophil migration and host defense (8).

  1. McKnight, A.J. and S. Gordon (1998) J Leukoc. Biol. 63:271.
  2. Stacey, M. et al. (2000) Trends Biochem. Sci. 25:284.
  3. Stacey, M. et al. (2003) Blood 102:2916.
  4. Hamann, J. et al. (2000) Int. Immunol. 12:439.
  5. Qian, Y.-M. et al. (1999) Immunology 98:303.
  6. Jaspars, L.H. et al. (2001) Tissue Antigens 57:325.
  7. Van Pel, M. et al. (2008) Haematologica 93:1137.
  8. Leemans, J.C. et al. (2004) J. Immunol. 172:1125.
Entrez Gene IDs
976 (Human); 26364 (Mouse)
Alternate Names
CD97; TM7LN1

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Citations for Mouse CD97 isoform 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Shear stress-dependent downregulation of the adhesion-G protein-coupled receptor CD97 on circulating leukocytes upon contact with its ligand CD55.
    Authors: Karpus O, Veninga H, Hoek R, Flierman D, van Buul J, Vandenakker C, vanBavel E, Medof M, van Lier R, Reedquist K, Hamann J
    J Immunol, 2013;190(7):3740-8.
  2. Analysis of CD97 expression and manipulation: antibody treatment but not gene targeting curtails granulocyte migration.
    Authors: Veninga H, Becker S, Hoek RM, Wobus M, Wandel E, van der Kaa J, van der Valk M, de Vos AF, Haase H, Owens B, van der Poll T, van Lier RA, Verbeek JS, Aust G, Hamann J
    J. Immunol., 2008;181(9):6574-83.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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