Mouse CMG-2/ANTXR2 Antibody Summary
Accession # Q6DFX2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse CMG‑2/ANTXR2 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, mouse thymus tissue, and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse CMG-2/ANTXR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3636) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CMG-2/ANTXR2 at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed anthrax toxin receptor (ATR) family protein (1‑4). CMG-2 is a ~55 kDa protein that contains a 31 amino acid (aa) signal sequence, a 287 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 148 aa cytoplasmic domain. Unlike human CMG-2 which has four isoforms, only one sequence has been reported for mouse CMG-2. The main functional domain of CMG‑2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS), through which it adheres selectively to collagen type IV and laminin (1‑6). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (4). In humans, CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (7). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (6, 8, 9). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (10, 11), allows PA pore formation and release of toxin to the cytoplasm (11, 12). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, mouse CMG-2 shares 84%, 91%, 80%, and 83% amino acid sequence homology with human, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa identity with ATR/TEM8.
- Scobie, H.M. and J.A.T. Young (2005), Curr. Opin. Microbiol. 8:106.
- Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
- Swiss Prot. Accession #: Q6DFX2.
- Bell, S.E. et al. (2001) J. Cell Sci. 114:2755.
- Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367.
- Santelli, E. et al. (2004) Nature 430:905.
- Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957.
- Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349.
- Go, M.Y. et al. (2006) J. Mol. Biol. 360:145.
- Abrami, L. et al. (2006) J. Cell Biol. 172:309.
- Wei, W. et al. (2006) Cell 124:1141.
- Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.
Citation for Mouse CMG-2/ANTXR2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
J. Immunol., 2009;183(9):5799-806.
Sample Types: Cell Lysates
Applications: Western Blot
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