Mouse Coagulation Factor VII Antibody Summary
Accession # P70375
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Coagulation Factor VII by Western Blot. Western blot shows lysate of mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Coagulation Factor VII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3305) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Coagulation Factor VII at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor VII
Coagulation Factors VII and VIIa refer to the pro and active forms of the same protease, respectively (1). Factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF), an integral membrane protein found in a variety of cell types. Upon binding of TF, factor VII is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiogenesis (2). The cleavage and activation of Coagulation Factors VII, IX and X by VIIa:TF is phospholipid-dependent whereas the cleavage of small peptide substrates is not (1). The deduced amino acid sequence of mouse factor VII predicts a signal peptide (residues 1 to 24), propeptide (residues 25 to 41), and the mature chain that can be further processed into the light chain (residues 42 to 193) and the heavy chain (residues 194 to 446). The amino acid sequence of mouse Factor VII is 89%, 71%, 65% and 55% identical to that of rat, human/dog, chimpanzee and chicken.
- Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. (eds. ), Academic Press, San Diego, p. 1659.
- Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
Citations for Mouse Coagulation Factor VII Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Chimeric antigen receptor-modified T Cells inhibit the growth and metastases of established tissue factor-positive tumors in NOG mice
Authors: Q Zhang, H Wang, H Li, J Xu, K Tian, J Yang, Z Lu, J Zheng
Sample Types: Whole Cells
Applications: Flow Cytometry
Targeting tissue factor on tumour cells and angiogenic vascular endothelial cells by factor VII-targeted verteporfin photodynamic therapy for breast cancer in vitro and in vivo in mice.
Authors: Hu Z, Rao B, Chen S, Duanmu J
BMC Cancer, 2010;10(0):235.
Sample Types: Whole Cells
Applications: ELISA Development
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