Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CRELD2
Ser23-Leu350
Accession # Q9CYA0

Specificity

Detects mouse CRELD2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CRELD2 is observed and less than 1% cross-reactivity with recombinant mouse CRELD1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CRELD2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CRELD2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRELD2

CRELD2 is a ubiquitously expressed protein that is the second member of the CRELD family of matricellular proteins. Mouse CRELD2 is synthesized as a 350 amino acid precursor protein with a signal peptide, a highly conserved domain rich in glutamic acid and tryptophan (WE), and EGF-like repeats. Human CRELD2 has been shown to interact with the alpha 4 subunit of the nicotinic acetylcholine receptor and function as a specific regulator of receptor expression. Mouse CRELD2 shares 77% amino acid sequence homology with the human CRELD2 alpha isoform.

Long Name

Cysteine-rich Protein With EGF-like Domains 2

Alternate Names

cysteine-rich with EGF-like domain protein 2, cysteine-rich with EGF-like domains 2, DKFZp667O055, MGC11256

Entrez Gene IDs

79174 (Human); 76737 (Mouse)

Gene Symbol

CRELD2

UniProt

Additional CRELD2 Products

Product Documents for Mouse CRELD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CRELD2 Antibody

For research use only

Citations for Mouse CRELD2 Antibody

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