Mouse ECM1 Antibody

Catalog # Availability Size / Price Qty
AF4428
AF4428-SP
Detection of Mouse ECM‑1 by Western Blot.
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Mouse ECM1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ECM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human ECM-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ECM-1
Ala20-Glu559
Accession # AAI38694
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse ECM‑1 antibody by Western Blot. View Larger

Detection of Mouse ECM‑1 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse ECM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4428) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ECM-1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ECM1

Extracellular matrix protein-1 (ECM-1, ECM-1a) is an 85 kDa, secreted glycoprotein important in connective tissue organization (1‑3). Of identified splice variants, the 559 amino acid (aa) form, ECM-1a is most widely expressed, with highest expression in the placenta, heart, and developing bones (3, 4). ECM-1b (434 aa) is found only in tonsil and skin, where it is associated with suprabasal keratinocytes (3, 5). Mouse ECM-1 contains a 19 aa signal peptide and a 540 aa secreted portion that includes an N-terminal proline-rich, cysteine-free region, two tandem repeat domains, and a C-terminal domain. Mature mouse ECM-1 shares 90% aa identity with rat ECM-1 and 65‑69% aa identity with corresponding isoforms of human, equine, bovine and canine ECM-1. There are six repeats of a CC(X7‑10)C motif (x = any aa) within the tandem repeat and C-terminal domains. These motifs, also found in members of the albumin family, are expected to form two (in ECM-1b) or three (in ECM‑1a) “double loop” structures that are involved in ligand binding to extracellular matrix molecules such as fibulin-1, perlecan, laminin 332, and fibronectin (4‑7). ECM-1 is over-expressed in many malignant epithelial tumors and has demonstrated angiogenic activity (8, 9). A role in regulating alkaline phosphatase during endochondral bone formation has also been suggested (4). In humans, loss of function within the tandem repeat regions due to mutation is considered causative of thickened and irregular extracellular matrix within connective tissue, called lipoid proteinosis (10). Autoantibodies in the skin disease lichen sclerosis also target these repeats (11). The phenotypes of these diseases support a role for ECM-1 as a “biological glue” in the dermis (1, 6, 7).

References
  1. Chan, I. (2004) Exp. Dermatol. 29:52.
  2. Bhalerao, J. et al. (1995) J. Biol. Chem 270:16385.
  3. Smits, P. et al. (1997) Genomics 45:487.
  4. Deckers, M.M.L. et al. (2001) Bone 28:14.
  5. Smits, P. et al. (2000) J. Invest. Dermatol. 114:718.
  6. Fujimoto, N. et al. (2005) Biochem. Biophys. Res. Commun. 333:1327.
  7. Sercu, S. et al. (2008) J. Invest. Dermatol. 128:1397.
  8. Han, Z. et al. (2001) FASEB J. 15:988.
  9. Wang, L. et al. (2003) Cancer Lett. 200:57.
  10. Hamada, T. et al. (2003) J. Invest. Dermatol. 120:345.
  11. Oyama, N. et al. (2004) J. Clin. Invest. 113:1550.
Long Name
Extracellular Matrix Protein 1
Entrez Gene IDs
1893 (Human); 13601 (Mouse); 116662 (Rat)
Alternate Names
ECM1; extracellular matrix protein 1; p85; Secretory Component Glycoprotein; Secretory Component P85

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