Mouse Endoglin/CD105 Antibody
Mouse Endoglin/CD105 Antibody Summary
Glu21-Gly581 (predicted)
Accession # NP_031958
Applications
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Endoglin/CD105 in Mouse Embryo. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (13.5 d.p.c.) using Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells of the developing hindlimb. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Image depicts staining at 10X magnification.
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Endoglin/CD105 in Mouse Embryo. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (13.5 d.p.c.) using Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells of the developing hindlimb. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Image depicts staining at 40X magnification.
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Endoglin/CD105 in Mouse Mesenchymal Stem Cells. Endoglin/CD105 was detected in immersion fixed mouse mesenchymal stem cells using Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endoglin/CD105
Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal, and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP-7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5 but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to anti-angiogenic activity (15).
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- ten Dijke, P. et al. (2008) Angiogenesis 11:79.
- Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
- Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
- Moody, J.L. et al. (2007) Stem Cells 25:2809.
- Velasco, S. et al. (2008) J. Cell Sci. 121:913.
- Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
- Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
- Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
- Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
- Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
- Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
- Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
- Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.
- Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Product Datasheets
Citations for Mouse Endoglin/CD105 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Release of STK24/25 suppression on MEKK3 signaling in endothelial cells confers Cerebral cavernous malformation
Authors: X Yang, ST Wu, R Gao, R Wang, Y Wang, Z Dong, L Wang, C Qi, X Wang, ML Schmitz, R Liu, Z Han, L Wang, X Zheng
JCI Insight, 2023;0(0):.
Species: Mouse
Sample Types: Embryo
Applications: IHC -
Immature Vascular Smooth Muscle Cells in Healthy Murine Arteries and Atherosclerotic Plaques: Localization and Activity
Authors: A Balatskiy, I Ozhimalov, M Balatskaya, A Savina, J Filatova, N Kalinina, V Popov, V Tkachuk
International Journal of Molecular Sciences, 2022;23(3):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Gli1+ Cells Couple with Type H Vessels and Are Required for Type H Vessel Formation
Authors: J Chen, M Li, AQ Liu, CX Zheng, LH Bao, K Chen, XL Xu, JT Guan, M Bai, T Zhou, BD Sui, DH Li, Y Jin, CH Hu
Stem Cell Reports, 2020;15(1):110-124.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Volume expansion and TRPV4 activation regulate stem cell fate in three-dimensional microenvironments
Authors: HP Lee, R Stowers, O Chaudhuri
Nat Commun, 2019;10(1):529.
Species: Mouse
Sample Types: Hydrogel Cells
Applications: IHC-Fr -
Gli1(+) Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target
Authors: RK Schneider, A Mullally, A Dugourd, F Peisker, R Hoogenboez, PMH Van Strien, EM Bindels, D Heckl, G Büsche, D Fleck, G Müller-New, J Wongboonsi, M Ventura Fe, VG Puelles, J Saez-Rodri, BL Ebert, BD Humphreys, R Kramann
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Bistable Epigenetic States Explain Age-Dependent Decline in Mesenchymal Stem Cell Heterogeneity
Authors: Z Hamidouche, K Rother, J Przybilla, A Krinner, D Clay, L Hopp, C Fabian, A Stolzing, H Binder, P Charbord, J Galle
Stem Cells, 2017;35(3):694-704.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Mesenchymal stem cells originating from ES cells show high telomerase activity and therapeutic benefits.
Authors: Ninagawa N, Murakami R, Isobe E, Tanaka Y, Nakagawa H, Torihashi S
Differentiation, 2011;82(3):153-64.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Selection -
PIR-B-deficient mice are susceptible to Salmonella infection.
Authors: Torii I, Oka S, Hotomi M, Benjamin WH, Takai T, Kearney JF, Briles DE, Kubagawa H
J. Immunol., 2008;181(6):4229-39.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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Endoglin/CD105 was detected 4% PFA fixed mouse brain. Brain sections were cutted with an Vibratom. Endoglin detection was performed with Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 20 µg/mL overnight at 4 °C. Brain sections were afterwards stained with AlexaFluor 647 coupled Anti-Rat IgG Secondary Antibody and counterstained with Hoechst 33342 (blue). Specific staining was localized to endothelial cells in Striatum of mouse brain. Transduced Neurons Express GFP.
