Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Frizzled-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
His23-Val157
Accession # Q61086

Specificity

Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Frizzled‑3 Antibody

Frizzled-3 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Frizzled‑3 in Mouse Embryo.

Frizzled-3 was detected in immersion fixed frozen sections of mouse embryo (E11) using Mouse Frizzled-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1001) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Frizzled‑3 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E11)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Frizzled-3

Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.

Alternate Names

Frizzled3, FZD3

Entrez Gene IDs

7976 (Human); 14365 (Mouse)

Gene Symbol

FZD3

UniProt

Q61086

Additional Frizzled-3 Products

Product Documents for Mouse Frizzled‑3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Frizzled‑3 Antibody

For research use only

Citations for Mouse Frizzled‑3 Antibody

Customer Reviews for Mouse Frizzled‑3 Antibody

There are currently no reviews for this product. Be the first to review Mouse Frizzled‑3 Antibody and earn rewards!

Have you used Mouse Frizzled‑3 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies