Mouse Frizzled-3 Antibody

Catalog # Availability Size / Price Qty
AF1001
AF1001-SP

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Frizzled‑3 in Mouse Embryo.
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Product Details
Citations (5)
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Mouse Frizzled-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
His23-Val157
Accession # Q61086
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Frizzled-3 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Frizzled‑3 in Mouse Embryo. Frizzled-3 was detected in immersion fixed frozen sections of mouse embryo (E11) using Mouse Frizzled-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1001) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-3

Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.

Entrez Gene IDs
7976 (Human); 14365 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); Frizzled3; Frizzled-3; Fz-3; FZD3; hFz3

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Citations for Mouse Frizzled-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Planar cell polarity signaling components are a direct target of beta-amyloid-associated degeneration of glutamatergic synapses
    Authors: B Feng, AE Freitas, L Gorodetski, J Wang, R Tian, YR Lee, AS Grewal, Y Zou
    Science Advances, 2021-08-18;7(34):.
    Applications: IHC
  2. SNX27 Deletion Causes Hydrocephalus by Impairing Ependymal Cell Differentiation and Ciliogenesis
    Authors: Xin Wang
    J. Neurosci, 2016-12-14;36(50):12586-12597.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.
    Authors: Shi, Yuqiang, Shu, Jianhong, Gelman, Benjamin, Lisinicchia, Joshua G, Tang, Shao-Jun
    J Neuroimmune Pharmacol, 2013-06-05;8(4):956-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Wnt5a regulates midbrain dopaminergic axon growth and guidance.
    Authors: Blakely BD, Bye CR, Fernando CV
    PLoS ONE, 2011-03-31;6(3):e18373.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3- and c-Jun N-terminal kinase-dependent mechanism.
    Authors: Endo Y, Beauchamp E, Woods D, Taylor WG, Toretsky JA, Uren A, Rubin JS
    Mol. Cell. Biol., 2008-01-22;28(7):2368-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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