Mouse Glypican 6 Antibody

Catalog #: AF1053
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1053
AF1053-SP

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Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Glypican 6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Glypican 6 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Glypican 6
Asp24-Val513
Accession # Q9R087

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Glypican 6

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo (E13)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 6

Glypican 6 (glypiated proteoglycan 6; also GPC6) is an 85-95 kDa heparan sulfate proteoglycan member of the GPI-linked glypican family of molecules. It is expressed by postnatal chrondrocytes and embryonic mesenchyme (connective tissue plus smooth muscle cells), and potentially modulates the availability, or signaling, of heparin-binding growth factors. Mouse Glypican 6 is synthesized as a 532 amino acid (aa) proprecursor that contains a 507 mature region (aa 4-530) and a 25 aa C-terminal prosegment. There is one potential splice variant that shows a 10 aa insertion after Leu384. Over aa 4-513, mouse Glypican 6 shares 99% aa identity with both human and rat Glypican 6.

Entrez Gene IDs

10082 (Human); 23888 (Mouse)

Alternate Names

Glypican 6; glypican proteoglycan 6; glypican-6; GPC6; MGC126288; OMIMD1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse Glypican 6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

GDE2 is essential for neuronal survival in the postnatal mammalian spinal cord
Authors: C Cave, S Park, M Rodriguez, M Nakamura, A Hoke, M Pletnikov, S Sockanatha
Mol Neurodegener, 2017;12(1):8.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot