Mouse Glypican 6 Antibody

Catalog # Availability Size / Price Qty
AF1053
AF1053-SP

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Detection of Mouse Glypican 6 by Western Blot
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Product Details
Citations (1)
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Mouse Glypican 6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Glypican 6 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Glypican 6
Asp24-Val513
Accession # Q9R087
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Glypican 6
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Glypican 6 by Western Blot View Larger

Detection of Mouse Glypican 6 by Western Blot GDE2 substrates show impaired release in SOD1G93A mice. a Schematic of the sequential solubilization protocol used to separate hydrophilic non-membrane proteins (S1) from hydrophobic membrane proteins (S2). b Western blot demonstrating the successful enrichment of non-membrane protein (GAPDH) in the S1 fraction and membrane protein (Na/K ATPase) in the S2 fraction for WT and SOD1G93A transgenic mice. c Western blots visualizing the impaired production of non-membrane bound (S1) Glypican 6, Glypican 4, and Glypican 1 in the lumbar spinal cord of 4 month SOD1G93A mice. d Quantification of the reduced membrane release of Glypican 6, *p = 0.038; Glypican 4, *p = 0.016; and Glypican 1, *p = 0.030 relative to GAPDH. Graph represents mean ± SEM. Student’s t test, n = 4 WT, 4 SOD1G93A. Image collected and cropped by CiteAb from the following open publication (https://molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-017-0148-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Glypican 6 by Western Blot View Larger

Detection of Mouse Glypican 6 by Western Blot GDE2 substrates show impaired release in SOD1G93A mice. a Schematic of the sequential solubilization protocol used to separate hydrophilic non-membrane proteins (S1) from hydrophobic membrane proteins (S2). b Western blot demonstrating the successful enrichment of non-membrane protein (GAPDH) in the S1 fraction and membrane protein (Na/K ATPase) in the S2 fraction for WT and SOD1G93A transgenic mice. c Western blots visualizing the impaired production of non-membrane bound (S1) Glypican 6, Glypican 4, and Glypican 1 in the lumbar spinal cord of 4 month SOD1G93A mice. d Quantification of the reduced membrane release of Glypican 6, *p = 0.038; Glypican 4, *p = 0.016; and Glypican 1, *p = 0.030 relative to GAPDH. Graph represents mean ± SEM. Student’s t test, n = 4 WT, 4 SOD1G93A Image collected and cropped by CiteAb from the following open publication (https://molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-017-0148-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 6

Glypican 6 (glypiated proteoglycan 6; also GPC6) is an 85-95 kDa heparan sulfate proteoglycan member of the GPI-linked glypican family of molecules. It is expressed by postnatal chrondrocytes and embryonic mesenchyme (connective tissue plus smooth muscle cells), and potentially modulates the availability, or signaling, of heparin-binding growth factors. Mouse Glypican 6 is synthesized as a 532 amino acid (aa) proprecursor that contains a 507 mature region (aa 4-530) and a 25 aa C-terminal prosegment. There is one potential splice variant that shows a 10 aa insertion after Leu384. Over aa 4-513, mouse Glypican 6 shares 99% aa identity with both human and rat Glypican 6.     

Entrez Gene IDs
10082 (Human); 23888 (Mouse)
Alternate Names
Glypican 6; glypican proteoglycan 6; glypican-6; GPC6; MGC126288; OMIMD1

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Citation for Mouse Glypican 6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. GDE2 is essential for neuronal survival in the postnatal mammalian spinal cord
    Authors: C Cave, S Park, M Rodriguez, M Nakamura, A Hoke, M Pletnikov, S Sockanatha
    Mol Neurodegener, 2017-01-19;12(1):8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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