Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Granzyme G Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Granzyme G
Ile21-Leu248
Accession # P13366

Specificity

Detects mouse Granzyme G in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant mouse Granzyme D is observed and less than 5% cross-reactivity with recombinant human Granzyme H and recombinant mouse Granzyme B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Granzyme G Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Granzyme G (Catalog # 1346-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Granzyme G (Catalog # 1346-SE)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Granzyme G

Granzyme G is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1). Together with Granzymes D, E and F, it is regulated through pregnancy and by IL-2 and IL-15 in granulated metrial gland cells (2). Human or rat counterpart of mouse Granzyme G has not been found. Like other Granzymes, mouse Granzyme G is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (3). It is synthesized as a 248 amino acid precursor with a 18 amino acid signal peptide and a 2 amino acid propeptide (3, 4). The mature protein (residues 21‑248) is expressed and purified.

References

  1. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  2. Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.
  3. Jenne, D.E. et al. (1989) Biochemistry 28:7953.
  4. Kwon, B.S. et al. (1988) J. Exp. Med. 168:1839.

Alternate Names

GrzG, GZMG

Entrez Gene IDs

14944 (Mouse); 266704 (Rat)

Gene Symbol

GZMG

UniProt

P13366

Additional Granzyme G Products

Product Documents for Mouse Granzyme G Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Granzyme G Antibody

For research use only

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