Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2923B
View all Hepassocin/FGL1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant mouse FGL-1
Leu23-Ile314
Accession # Q71KU9

Specificity

Detects mouse FGL-1 in Direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse Hepassocin/FGL1 Antibody

Detection of Hepassocin/FGL1 in Hepa 1‑6 Mouse Hepatoma Cell Line (Positive) and BaF3 Mouse Pro-B Cell Line (Negative) Cells.

Hepassocin/FGL1 was detected in immersion fixed Hepa 1‑6 Mouse Hepatoma Cell Line (Positive) and BaF3 Mouse Pro-B Cell Line (Negative) Cells using Rabbit Anti-Mouse Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB11247) at 8 µg/mL for 3 hours at room temperature. Hepa 1-6 cells were treated with 3uM Monensin for 3 hours. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Hepassocin/FGL1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Hepa 1‑6 Mouse Hepatoma Cell Line (Positive) and BaF3 Mouse Pro-B Cell Line (Negative) Cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein (HFREP-1) and Fibrinogen-like protein 1 (FGL-1), is a liver specific secreted protein containing one fibrinogen C-terminal domain. It exists as a homodimer and is reported to have hepatocyte mitogenic activity. Hepassocin/FGL-1 binds LAG3 independently from MHC class II (MHC-II) and acts as an Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3.

Long Name

Fibrinogen Like 1

Alternate Names

FGL1, Fibrinogen-like-protein 1, FREP1

Entrez Gene IDs

2267 (Human); 234199 (Mouse); 246186 (Rat); 102118020 (Cynomolgus Monkey)

Gene Symbol

FGL1

UniProt

Q71KU9 (Mouse)

Additional Hepassocin/FGL1 Products

Product Documents for Mouse Hepassocin/FGL1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Hepassocin/FGL1 Antibody

For research use only

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