Mouse Hip Antibody

Catalog # Availability Size / Price Qty
AF1568
AF1568-SP

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Product Details
Citations (4)
FAQs
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Mouse Hip Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Hip in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Hip
Lys24-Arg678 (Asn29Ser)
Accession # Q7TN16
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Hip (Catalog # 1568-HP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hip

Hedgehog signaling proteins act as mitogens, morphogens, or inducing factors in many different cell types during embryonic development. They aid in growth, patterning, and morphogenesis in both vertebrates and insects (1). Hip (Hedgehog-interacting protein) is a type I transmembrane protein identified for its ability to bind biologically active Sonic Hedgehog. It is comprised of 700 aa, and includes a hydrophobic signal sequence, two EGF-like domains near the C-terminus, and a 22 amino acid membrane-spanning region at the C-terminal end (2). Hip has only been identified in vertebrates and binds all three mammalian Hedgehogs: sonic (Shh), desert (Dhh), and Indian (Ihh). Like the Hedgehog receptor Patched, Hip is a transcriptional target of Hedgehog signaling (2). Unlike Patched, Hip’s ability to bind hedgehogs is not involved in transducing a signal intracellularly, rather it regulates the availability of Hedgehog ligand extracellularly (3). Transgenic mice overexpressing Hip in proliferating chondrocytes display skeletal defects similar to those observed in Ihh mutant mice. These results indicate that Hip is involved in attenuating Hedgehog signaling (2). The expression pattern of Hip correlates with its ability to interact with all three mammalian Hedgehogs. It is expressed in a variety of organs, adjacent with sites of hedgehog expression. For instance, Shh is expressed in the epithelium of the lung, and Hip is found in the underlying lung mesenchyme (2). In fact, Hip knock-out mice exhibit neonatal lethality with respiratory failure due to defective branching morphogenesis. This phenotype correlates with altered expression of Shh markers suggesting an increase in Shh signaling (3). Interestingly, other developmental mechanisms that rely on normal Shh signaling, such as dorsal-ventral patterning of the neural tube, development of the somites, and organ laterality appeared histologically normal in Hip-/- mice (3). Mouse and human Hip share 94% amino acid identity through the entire protein sequence (4). R&D Systems’ recombinant Hedgehog-interacting protein is a potent antagonist of Shh signaling in vitro.

References
  1. Ingham, P.W. and A.P. McMahon (2001) Genes & Dev 15:3059.
  2. Chuang, P-T. and A.P. McMahon (1999) Nature 397: 17.
  3. Chuang, P-T. et al. (2003) Genes & Dev. 17:342.
  4. Bak, M. et al. (2001) Cytogenet. Cell Genet. 92:300.
Long Name
Hedgehog-interacting Protein
Entrez Gene IDs
64399 (Human); 15245 (Mouse)
Alternate Names
FLJ90230; hedgehog interacting protein; hedgehog-interacting protein; HHIP; Hip; HIPFLJ20992

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Citations for Mouse Hip Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Distinct effects of ligand-induced PDGFRalpha and PDGFRbeta signaling in the human rhabdomyosarcoma tumor cell and stroma cell compartments.
    Authors: Ehnman M, Missiaglia E, Folestad E, Selfe J, Strell C, Thway K, Brodin B, Pietras K, Shipley J, Ostman A, Eriksson U
    Cancer Res, 2013-01-21;73(7):2139-49.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Down-regulation of hedgehog-interacting protein through genetic and epigenetic alterations in human hepatocellular carcinoma.
    Authors: Tada M, Kanai F, Tanaka Y, Tateishi K, Ohta M, Asaoka Y, Seto M, Muroyama R, Fukai K, Imazeki F, Kawabe T, Yokosuka O, Omata M
    Clin. Cancer Res., 2008-06-15;14(12):3768-76.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Phorbol esters inhibit the Hedgehog signalling pathway downstream of Suppressor of Fused, but upstream of Gli.
    Authors: Lauth M, Bergstrom A, Toftgard R
    Oncogene, 2007-02-19;26(35):5163-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Activation of the hedgehog pathway in a subset of lung cancers.
    Authors: Chi S, Huang S, Li C, Zhang X, He N, Bhutani MS, Jones D, Castro CY, Logrono R, Haque A, Zwischenberger J, Tyring SK, Zhang H, Xie J
    Cancer Lett., 2006-01-30;244(1):53-60.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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