Mouse HPRG Antibody

Catalog # Availability Size / Price Qty
AF1905
AF1905-SP
Product Details
Citations (1)
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Mouse HPRG Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse HPRG in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human HPRG is observed and less than 2% cross-reactivity wth recombinant mouse (rm) Cystatin A, rmCystatin B, rmCystatin C, and rmCystatin E/M is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse HPRG
Leu19-Lys525
Accession # BAB33094
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse HPRG (Catalog # 1905-HP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HPRG

Mouse histidine-rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67‑75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 22% of its amino acids (aa) are histidine and proline. In mouse, it is synthesized as a 525 amino acid (aa) precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N-terminal cystatin-like modules and one His-Pro-rich region that is flanked by two Pro-rich segments (3, 4). The His-Pro-rich region contains multiple tandem repeats with a GHHPH motif, while the N- and
C‑termini are linked by a disulfide bond (3, 5, 6). Mouse HPRG is only 60% aa identical to human HPRG, and 79% aa identical to rat HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a
Zn++‑dependent manner, it is converted to plasmin by tPA (7‑9). HPRG also shows antiangiogenic activity on endothelial cells (10). Finally, it binds to cytoplasmic ligand(s) exposed during cellular necrosis, and facilitates macrophage phagocytosis (11).

References
  1. Jones, A.L. et al. (2005) Immunol. Cell Biol. 83:106.
  2. Koide, T. and S. Odani (1987) FEBS Lett. 216:17.
  3. Hulett, M.D. and C.R. Parish (2000) Immunol. Cell Biol. 78:280.
  4. GenBank Accession # NP_444406.
  5. Borza, D-B. et al. (1996) Biochemistry 35:1925.
  6. Sorensen, C.B. et al. (1993) FEBS Lett. 328:285.
  7. Donate, F. et al. (2004) Cancer Res. 64:5812.
  8. Borza, D-B. and W.T. Morgan (1997) J. Biol. Chem. 272:5718.
  9. Guan, X. et al. (2004) Thromb. Haemost. 92:403.
  10. Juarez, J.C. et al. (2002) Cancer Res. 62:5344.
  11. Jones, A.L. et al. (2005) J. Biol. Chem. 280:35733.
Long Name
Histidine-rich Glycoprotein Precusor
Entrez Gene IDs
3273 (Human)
Alternate Names
DKFZp779H1622; Histidine-proline-rich glycoprotein; histidine-rich glycoprotein; HPRG; HPRGHRGP; HRG

Product Datasheets

Citation for Mouse HPRG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Differential proteomics analysis of the surface heterogeneity of dextran iron oxide nanoparticles and the implications for their in vivo clearance.
    Authors: Simberg D, Park JH, Karmali PP, Zhang WM, Merkulov S, McCrae K, Bhatia SN, Sailor M, Ruoslahti E
    Biomaterials, 2009;30(23):3926-33.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot

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