Mouse ICOS Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB1682-100
MAB1682-SP
Mouse ICOS Antibody in Flow Cytometry
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Mouse ICOS Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ICOS in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2317C
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line, CHO-derived mouse ICOS
Met1-Leu142
Accession # Q9WVS0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 7.5-45 ng/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Mouse ICOS Fc Chimera to immobilized Recombinant Mouse B7-H2 (Catalog # 8127-B7) coated at 1 µg/mL (100 µL/well). At 100 ng/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of ICOS in HEK293 Human Cell Line Transfected with Mouse ICOS and eGFP by Flow Cytometry. View Larger

Detection of ICOS in HEK293 Human Cell Line Transfected with Mouse ICOS and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse ICOS or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Mouse ICOS Monoclonal Antibody (Catalog # MAB1682) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICOS

Mouse ICOS (inducible co-stimulator), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Mouse ICOS is a homodimeric type I transmembrane protein consisting of 200 amino acids (aa) with a putative 20 aa signal sequence, a 122 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% aa similarity with CD 28 and CTLA-4. Mouse and human ICOS share approximately 72% aa identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is upregulated within approximately 24‑48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

References
  1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  2. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  3. Yoshinaga, S.K. et al. (1999) Nature 402:827.
  4. Hutloff, A. et al. (1999) Nature 397:263.
  5. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  6. Coyle, A.J. et al. (2000) Immunity 13:95.
  7. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
Long Name
Inducible Co-stimulator
Entrez Gene IDs
29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names
Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850

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