Mouse IFN-alpha / beta R2 Antibody

AF1083 3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1083
AF1083-SP

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Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R2 Antibody.

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IFN-alpha / beta R2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IFN‑ alpha / beta R2 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IFN-gamma R2 and rmIL-20 R alpha is observed, 5% cross-reactivity with recombinant human (rh) IL-10 R beta, rmIFN-gamma RI, and rhIL-20 R beta is observed and less than 1% cross-reactivity with rhIFN-beta R alpha is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta R2
Ser22-Ala239 (Lys160Asn)
Accession # Q9D1R7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IFN-alpha / beta R2

Neutralization

Measured by its ability to neutralize IFN‑ alpha / beta R2-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 2-10 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization

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Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R2 Antibody. Recombinant Mouse Limitin (Catalog # 1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN-a/ beta R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1083). The ND₅₀ is typically 2-10 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R2

IFN-alpha / beta R2 belongs to the type II cytokine receptor family. It complexes with IFN-alpha / beta R1 to form the signaling receptor complex for the family of alpha and beta IFN subtypes. By alternative splicing, IFN-alpha / beta R2 can exist as a secreted soluble protein or as a type I membrane protein.

Long Name

Interferon alpha/beta Receptor 2

Entrez Gene IDs

3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)

Alternate Names

beta receptor10; human interferon alpha; human interferon alpha/beta receptor; IFNABR; IFN-alpha binding protein; IFN-alpha/beta R2; IFN-alpha/beta receptor 2; IFN-alpha-REC; IFNAR2; IFN-aR2; IFNARB; IFNbR2; IFN-bR2; IFN-R; IFN-R-2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IFN-alpha / beta R2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Central nervous system regeneration is driven by microglia necroptosis and repopulation
Authors: AF Lloyd, CL Davies, RK Holloway, Y Labrak, G Ireland, D Carradori, A Dillenburg, E Borger, D Soong, JC Richardson, T Kuhlmann, A Williams, JW Pollard, A des Rieux, J Priller, VE Miron
Nat. Neurosci., 2019;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Virus-Triggered ATP Release Limits Viral Replication through Facilitating IFN-? Production in a P2X7-Dependent Manner
Authors: C Zhang, H He, L Wang, N Zhang, H Huang, Q Xiong, Y Yan, N Wu, H Ren, H Han, M Liu, M Qian, B Du
J. Immunol., 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Differential Growth of IFN-beta-Engineered Tumor Cells in Nude and IFN Receptor-Null Mice.
Authors: Zhang F, Lee J, Wang D, Dong Z
J. Interferon Cytokine Res., 2006;26(2):108-18.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot