Detects mouse IFN‑ alpha / beta R2 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IFN-gamma R2 and rmIL-20 R alpha is observed, 5% cross-reactivity with recombinant human (rh) IL-10 R beta, rmIFN-gamma RI, and rhIL-20 R beta is observed and less than 1% cross-reactivity with rhIFN-beta R alpha is observed.
Measured by its ability to neutralize IFN‑ alpha / beta R2-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R2 Antibody.
Recombinant Mouse Limitin (Catalog # 1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN‑ alpha / beta R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1083). The ND50 is typically 2-10 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-alpha/beta R2
IFN-alpha / beta R2 belongs to the type II cytokine receptor family. It
complexes with IFN-alpha / beta R1 to form the signaling receptor complex for
the family of alpha and beta IFN subtypes. By alternative splicing, IFN-alpha / beta R2
can exist as a secreted soluble protein or as a type I membrane protein.
Interferon alpha/beta Receptor 2
Entrez Gene IDs:
3455 (Human); 15976 (Mouse)
beta receptor10; human interferon alpha; human interferon alpha/beta receptor; IFNABR; IFN-alpha binding protein; IFN-alpha/beta R2; IFN-alpha/beta receptor 2; IFN-alpha-REC; IFNAR2; IFN-aR2; IFNARB; IFNbR2; IFN-bR2; IFN-R; IFN-R-2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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