Protein A or G purified from cell culture supernatant
Human embryonic kidney cell line HEK293-derived mouse IFN‑ beta Ile22-Asn182 Accession # P01575
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize IFN‑ beta inhibition of viral-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 10 pg/mL Recombinant Mouse IFN‑ beta.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IFN‑ beta Inhibition of Viral-induced Cytopathy and Neutralization by Mouse IFN‑ beta Antibody.
Recombinant Mouse IFN‑ beta (Catalog # 8234-MB) reduces the viral-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Inhibition of viral activity elicited by Recombinant Mouse IFN‑ beta (10 pg/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse IFN‑ beta Monoclonal Antibody (Catalog # MAB8234). The ND50 is typically 0.25-1.25 µg/mL.
Preparation and Storage
at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).
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