Mouse IL-15 Biotinylated Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-15 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human IL‑15 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse IL-15
    Asn49-Ser162
    Accession # P48346
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑15 (Catalog # 447-ML)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Mouse IL-15 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse IL‑15 Antibody (Catalog # MAB447)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse IL‑15 Biotinylated Antibody (Catalog # BAF447)
  • ELISA Standard
     
    Recombinant Mouse IL-15 Protein (Catalog # 447-ML)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
IL‑15 in Mouse Intestine. IL‑15 was detected in perfusion fixed frozen sections of mouse intestine using Mouse IL‑15 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF447) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
IL‑15 in Mouse Intestine. IL‑15 was detected in perfusion fixed frozen sections of mouse intestine using Mouse IL‑15 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF447) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-15
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1 - 3). Mature mouse IL-15 shares 70% and 96% amino acid sequence identity with human and rat IL-15, respectively. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11 - 13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
  • References:
    1. Grabstein, K. et al. (1994) Science 264:965.
    2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
    3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
    4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
    5. Giri, J.G. et al. (1995) EMBO J. 14:3654.
    6. Giri, J. et al. (1994) EMBO J. 13:2822.
    7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
    8. Dubois, S. et al. (2002) Immunity 17:537.
    9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
    10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
    11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
    12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
    13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
    14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
    15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
  • Long Name:
    Interleukin 15
  • Entrez Gene IDs:
    3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
  • Alternate Names:
    IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes.
    Authors: Pandiyan P, Yang X, Saravanamuthu S, Zheng L, Ishihara S
    J. Immunol., 2012;189(9):4237-46.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  2. IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity.
    Authors: Sikora AG, Jaffarzad N, Hailemichael Y, Gelbard A, Stonier SW, Schluns KS, Frasca L, Lou Y, Liu C, Andersson HA, Hwu P, Overwijk WW
    J. Immunol., 2009;182(12):7398-407.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in trans.
    Authors: Rowley J, Monie A, Hung CF, Wu TC
    J. Immunol., 2008;181(12):8237-47.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation.
    Authors: Mortier E, Woo T, Advincula R, Gozalo S, Ma A
    J. Exp. Med., 2008;205(5):1213-25.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ELISA Development
  5. IL-12 rapidly alters the functional profile of tumor-associated and tumor-infiltrating macrophages in vitro and in vivo.
    Authors: Watkins SK, Egilmez NK, Suttles J, Stout RD
    J. Immunol., 2007;178(3):1357-62.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
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