Mouse IL-15 Antibody

Catalog # Availability Size / Price Qty
MAB447-SP
MAB447-500
MAB447-100
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Mouse IL-15 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-15 in ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) IL‑2, rhIL‑15, or rmIL-21.
Source
Monoclonal Rat IgG2A Clone # 201136
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-15
Asn49-Ser162
Accession # P48346
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑15 (Catalog # 447-ML)

Mouse IL-15 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑15 Biotinylated Antibody (Catalog # BAF447)

Standard: Recombinant Mouse IL-15 Protein (Catalog # 447-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1 - 3). Mature mouse IL-15 shares 70% and 96% amino acid sequence identity with human and rat IL-15, respectively. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11 - 13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

References
  1. Grabstein, K. et al. (1994) Science 264:965.
  2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
  3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. 94:14444.
  5. Giri, J.G. et al. (1995) EMBO 14:3654.
  6. Giri, J. et al. (1994) EMBO J. 13:2822.
  7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
  8. Dubois, S. et al. (2002) Immunity 17:537.
  9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
  10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
  14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
Long Name
Interleukin 15
Entrez Gene IDs
3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names
IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15

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Citations for Mouse IL-15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Effect of treatment with conditioned media derived from C2C12 myotube on adipogenesis and lipolysis in 3T3-L1 adipocytes
    Authors: K Tamura, N Goto-Inoue, K Miyata, Y Furuichi, NL Fujii, Y Manabe
    PLoS ONE, 2020-08-05;15(8):e0237095.
    Species: Mouse
    Sample Types: Cell Culture Lysates, Cell Lysates
    Applications: Western Blot
  2. Evidence for acute contraction-induced myokine secretion by C2C12 myotubes
    Authors: Y Furuichi, Y Manabe, M Takagi, M Aoki, NL Fujii
    PLoS ONE, 2018-10-24;13(10):e0206146.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes.
    Authors: Pandiyan P, Yang X, Saravanamuthu S, Zheng L, Ishihara S
    J. Immunol., 2012-09-19;189(9):4237-46.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  4. IL-12 rapidly alters the functional profile of tumor-associated and tumor-infiltrating macrophages in vitro and in vivo.
    Authors: Watkins SK, Egilmez NK, Suttles J, Stout RD
    J. Immunol., 2007-02-01;178(3):1357-62.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

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Reviews for Mouse IL-15 Antibody

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Mouse IL‑15 Antibody
By Katie Myers on 11/14/2022
Application: ELISA Sample Tested: Serum Species: Mouse

We tried using this as a capture antibody in an ELISA. It gave very little signal as compared to our other analytes.