Mouse IL‑15 Antibody

Detection of Mouse IL-15 by Western Blot

Eugenol promotes IL-15 level through TRPV1-mediated CaN/NFATc1 signaling pathway.C2C12 myotubes were treated by 0–200 eugenol for 1 day after 4 days of differentiation. (A) The effect of eugenol on Il15 mRNA expression in C2C12 myotubes. (B) The effect of eugenol on IL-15 protein expression in the C2C12 cell medium. Coomassie staining as loading control. (C, D) C2C12 myotubes were treated by 25 μM eugenol & 1 μM TRPV1 inhibitor AMG-517 or 0.5 μM calcineurin (CaN) inhibitor cyclosporine A (CsA) for 1 day after 4 days of differentiation. The mRNA & protein expression of IL-15. N=4 per group. One-way ANOVA test was used to determine statistical significance. *p<0.05, **p<0.01, & ***p<0.001.Figure 9—source data 1.IL-15 mRNA expression in C2C12 myotubes (Figure 9A).Figure 9—source data 2.Original files for the WB analysis (Figure 9B).Figure 9—source data 3.PDF containing Figure 9B & original scans of the relevant WB analysis, with cropped areas. Figure 9—source data 4.IL-15 mRNA expression in C2C12 myotubes (Figure 9C).Figure 9—source data 5.Original files for the WB analysis (Figure 9D).Figure 9—source data 6.PDF containing Figure 9D & original scans of the relevant WB analysis, with cropped areas.IL-15 mRNA expression in C2C12 myotubes (Figure 9A).Original files for the WB analysis (Figure 9B).PDF containing Figure 9B & original scans of the relevant WB analysis, with cropped areas.IL-15 mRNA expression in C2C12 myotubes (Figure 9C).Original files for the WB analysis (Figure 9D).PDF containing Figure 9D & original scans of the relevant WB analysis, with cropped areas. Representative immunofluorescence images of IL-15.IL-15 (green fluorescence) & 4,6-diamidino-2-phenylindole (DAPI) (blue fluorescence). Magnification: ×200. Image collected & cropped by CiteAb from the following open publication (https://elifesciences.org/articles/90724), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse IL-15 by Western Blot

Eugenol promotes the expression and secretion of IL-15 in skeletal muscle of mice. (E) The protein expression of slow myosin heavy chain (MyHC), fast MyHC, and IL-15. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/90724), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse IL-15 by Western Blot

The myokine IL-15 expression depends on CaN/NFATc1 signaling pathway. (B–D) C2C12 myotubes were treated by 0.5 μM A23187 and 0.5 μM cyclosporine A (CsA) for 16 hr after 2 days of differentiation. The protein expression of CnA, NFATc1, and IL-15. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/90724), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse IL-15 by Western Blot

Eugenol promotes IL-15 level through TRPV1-mediated CaN/NFATc1 signaling pathway.C2C12 myotubes were treated by 0–200 eugenol for 1 day after 4 days of differentiation. (A) The effect of eugenol on Il15 mRNA expression in C2C12 myotubes. (B) The effect of eugenol on IL-15 protein expression in the C2C12 cell medium. Coomassie staining as loading control. (C, D) C2C12 myotubes were treated by 25 μM eugenol & 1 μM TRPV1 inhibitor AMG-517 or 0.5 μM calcineurin (CaN) inhibitor cyclosporine A (CsA) for 1 day after 4 days of differentiation. The mRNA & protein expression of IL-15. N=4 per group. One-way ANOVA test was used to determine statistical significance. *p<0.05, **p<0.01, & ***p<0.001.Figure 9—source data 1.IL-15 mRNA expression in C2C12 myotubes (Figure 9A).Figure 9—source data 2.Original files for the WB analysis (Figure 9B).Figure 9—source data 3.PDF containing Figure 9B & original scans of the relevant WB analysis, with cropped areas. Figure 9—source data 4.IL-15 mRNA expression in C2C12 myotubes (Figure 9C).Figure 9—source data 5.Original files for the WB analysis (Figure 9D).Figure 9—source data 6.PDF containing Figure 9D & original scans of the relevant WB analysis, with cropped areas.IL-15 mRNA expression in C2C12 myotubes (Figure 9A).Original files for the WB analysis (Figure 9B).PDF containing Figure 9B & original scans of the relevant WB analysis, with cropped areas.IL-15 mRNA expression in C2C12 myotubes (Figure 9C).Original files for the WB analysis (Figure 9D).PDF containing Figure 9D & original scans of the relevant WB analysis, with cropped areas. Representative immunofluorescence images of IL-15.IL-15 (green fluorescence) & 4,6-diamidino-2-phenylindole (DAPI) (blue fluorescence). Magnification: ×200. Image collected & cropped by CiteAb from the following open publication (https://elifesciences.org/articles/90724), licensed under a CC-BY license. Not internally tested by R&D Systems.