Mouse IL-23R Antibody

Catalog # Availability Size / Price Qty
AF1686
AF1686-SP
Product Details
Citations (5)
FAQs
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Mouse IL-23R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-23 R in direct ELISAs and Western blots. In these formats, approximately 15% cross‑reactivity with recombinant human IL‑23 R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-23 R
Gly24-Asp372
Accession # Q8K4B4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑23 R Fc Chimera (Catalog # 1686-MR)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 40 ng/mL of Recombinant Mouse IL-23 (Catalog # 1887-ML) to immobilized Recombinant Mouse IL-23 R Fc Chimera (Catalog # 1686-MR) coated at 5 µg/mL (100 µL/well). At 30 µg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4+ CD45RBlow memory T cells, but at much lower levels by mouse CD4+ CD45RBhigh cells. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from IL-12.

References
  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5448.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Long Name
Interleukin 23 Receptor
Entrez Gene IDs
149233 (Human); 209590 (Mouse); 102133210 (Cynomolgus Monkey)
Alternate Names
IBD17; IL-23 R; IL-23 receptor; IL23R; IL-23R; interleukin 23 receptor; interleukin-23 receptor

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Citations for Mouse IL-23R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. EBV-induced gene 3 augments IL-23R&alpha protein expression through a chaperone calnexin
    Authors: I Mizoguchi, M Ohashi, H Hasegawa, Y Chiba, N Orii, S Inoue, C Kawana, M Xu, K Sudo, K Fujita, M Kuroda, SI Hashimoto, K Matsushima, T Yoshimoto
    J. Clin. Invest., 2020-11-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  2. Clinical and Histopathological Amelioration of Experimental Autoimmune Encephalomyelitis by AAV Vectors Expressing a Soluble Interleukin-23 Receptor
    Authors: M Miralles, H Eixarch, M Tejero, C Costa, K Hirota, AR Castaño, M Puig, G Stockinger, X Montalban, A Bosch, C Espejo, M Chillon
    Neurotherapeutics, 2017-10-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. IFN-?-Expressing Th17 Cells Are Required for Development of Severe Ocular Surface Autoimmunity
    Authors: Y Chen, SK Chauhan, C Shao, M Omoto, T Inomata, R Dana
    J. Immunol., 2017-06-21;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
    Authors: Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE
    J. Immunol., 2007-02-01;178(3):1341-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. IL-23 neutralization protects mice from Gram-negative endotoxic shock.
    Authors: Belladonna ML, Vacca C, Volpi C, Giampietri A, Fioretti MC, Puccetti P, Grohmann U, Campanile F
    Cytokine, 2006-06-08;34(3):161-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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