Mouse IL-4 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB404R-SP
MAB404R-100
MAB404R-500
MAB404R-01M

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Cell Proliferation Induced by IL-4 and Neutralization by Mouse IL-4 Antibody.
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Mouse IL-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-4 in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG1 Clone # 30340R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E.coli-derived recombinant mouse IL-4
His23-Ser140
Accession # P07750
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Mouse IL-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑4 Biotinylated Antibody (Catalog # BAF404)

Standard: Recombinant Mouse IL-4 Protein (Catalog # 404-ML)

Neutralization
Measured by its ability to neutralize IL‑4-induced proliferation in the HT‑2 mouse T cell line. The Neutralization Dose (ND50) is typically 0.1-0.6 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL-4 and Neutralization by Mouse IL-4 Antibody. View Larger

Cell Proliferation Induced by IL-4 and Neutralization by Mouse IL-4 Antibody. Recombinant Mouse IL-4 (Catalog # 404-ML) stimulates proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-4 Monoclonal Antibody (Catalog # MAB404R). The ND50 is typically 0.1-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11‑14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

References
  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Lee, F. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2061.
  4. Noma, Y. et al. (1986) Nature 319:640.
  5. Redfield, C. et al. (1991) Biochemistry 30:11029.
  6. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  7. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  8. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  9. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  10. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  11. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  12. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  13. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  14. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  15. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Interleukin 4
Entrez Gene IDs
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

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Mouse IL-4 Antibody
By Anonymous on 12/01/2017
Application: Flow Sample Tested: Small intestine tissue Species: Mouse