Mouse IL-6 Antibody

(39 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-6 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL‑6, recombinant porcine IL‑6, recombinant rat IL-6, rhIL-11, rhCT-1, or rhCLC is observed.
  • Source
    Monoclonal Rat IgG1 Clone # MP5-20F3
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse IL-6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
    • Mouse IL-6 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse IL‑6 Antibody (Catalog # MAB406)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse IL‑6 Biotinylated Antibody (Catalog # BAF406)
  • ELISA Standard
     
    Recombinant Mouse IL-6 Protein (Catalog # 406-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND50) is typically 0.005-0.025 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Mouse IL‑6 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL‑6 (Catalog #
406-ML), Recombinant Human IL‑6 (Catalog # 206-IL) and Recombinant Rat IL‑6 (Catalog # 506-RL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL‑6 Monoclonal Antibody (Catalog # MAB406) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL‑6 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody.

Recombinant Mouse IL‑6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑6 Monoclonal Antibody (Catalog # MAB406). The ND50 is typically 0.005-0.025 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature mouse IL-6 is 187 amino acids (aa) in length and shares 42% and 85% aa sequence identity with human and rat IL-6, respectively (5). Alternate splicing generates several isoforms with internal deletions (6). Mouse IL-6 is equally active on rat cells (7). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (8). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (9). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (9). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 10).

  • References:
    1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
    2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
    3. Jones, S.A. (2005) J. Immunol. 175:3468.
    4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
    5. Van Snick, J. et al. (1988) Eur. J. Immunol. 18:193.
    6. Yatsenko, O.P. et al. (2004) Cytokine 28:190.
    7. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. 85:7099.
    8. Murakami, M. et al. (1993) Science 260:1808.
    9. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
    10. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  • Long Name:
    Interleukin 6
  • Entrez Gene IDs:
    3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
  • Alternate Names:
    B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Capture
  2. Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6
    Authors: SI Ohkura, S Usui, SI Takashima, N Takuwa, K Yoshioka, Y Okamoto, Y Inagaki, N Sugimoto, T Kitano, M Takamura, T Wada, S Kaneko, Y Takuwa
    PLoS ONE, 2017;12(8):e0182329.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neutralzation
  3. Inhibitory effect ofquercetinon titaniumparticle-inducedendoplasmic reticulum stress (ERS)-related apoptosisandin vivoosteolysis
    Authors: L Zhang, Z Tian, W Li, X Wang, Z Man, S Sun
    Biosci. Rep., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  4. Notch Balances Th17 and Induced Regulatory T Cell Functions in Dendritic Cells by Regulating Aldh1a2 Expression
    Authors: TS Zaman, H Arimochi, S Maruyama, C Ishifune, SI Tsukumo, A Kitamura, K Yasutomo
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. IL6 Blockade Reprograms the Lung Tumor Microenvironment to Limit the Development and Progression of K-ras-Mutant Lung Cancer
    Authors: MS Caetano, H Zhang, AM Cumpian, L Gong, N Unver, EJ Ostrin, S Daliri, SH Chang, CE Ochoa, S Hanash, C Behrens, II Wistuba, C Sternberg, H Kadara, CG Ferreira, SS Watowich, SJ Moghaddam
    Cancer Res., 2016;76(11):3189-99.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  6. Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis
    Authors: Jung-Yeon Lim
    Sci Rep, 2016;6(0):26851.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  7. The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity
    Sci Rep, 2016;6(0):29994.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  8. KIM-1-mediated phagocytosis reduces acute injury to the kidney.
    Authors: Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, Ichimura T, Kuchroo V, Bonventre J
    J Clin Invest, 2015;125(4):1620-36.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ELISA Capture
  9. Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
    Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
    Nature, 2015;515(7525):130-3.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  10. Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
    Authors: Levy O, Calippe B, Lavalette S, Hu S, Raoul W, Dominguez E, Housset M, Paques M, Sahel J, Bemelmans A, Combadiere C, Guillonneau X, Sennlaub F
    EMBO Mol Med, 2015;7(2):211-26.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  11. Coexistent ARID1A-PIK3CA mutations promote ovarian clear-cell tumorigenesis through pro-tumorigenic inflammatory cytokine signalling.
    Authors: Chandler, Ronald L, Damrauer, Jeffrey, Raab, Jesse R, Schisler, Jonathan, Wilkerson, Matthew, Didion, John P, Starmer, Joshua, Serber, Daniel, Yee, Della, Xiong, Jessie, Darr, David B, Pardo-Manuel de Villena, Fernando, Kim, William, Magnuson, Terry
    Nat Commun, 2015;6(0):6118.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  12. Oxidative stress-induced inhibition of Sirt1 by caveolin-1 promotes p53-dependent premature senescence and stimulates the secretion of interleukin 6 (IL-6).
    Authors: Volonte D, Zou H, Bartholomew J, Liu Z, Morel P, Galbiati F
    J Biol Chem, 2015;290(7):4202-14.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  13. Basophils control T-cell responses and limit disease activity in experimental murine colitis.
    Authors: Gomez M, Talke Y, Hofmann C, Ketelsen I, Hermann F, Reich B, Goebel N, Schmidbauer K, Dunger N, Bruhl H, Renner K, Syed S, Mack M
    Mucosal Immunol, 2014;7(1):188-99.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  14. Autocrine interleukin-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca(2+) channels.
    Authors: Ke F, Zhang L, Liu Z, Liu J, Yan S, Xu Z, Bai J, Zhu H, Lou F, Wang H, Shi Y, Jiang Y, Su B, Wang H
    Stem Cells, 2014;32(10):2799-810.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  15. Stabilization and augmentation of circulating AIM in mice by synthesized IgM-Fc.
    Authors: Kai, Toshihir, Yamazaki, Tomoko, Arai, Satoko, Miyazaki, Toru
    PLoS ONE, 2014;9(5):e97037.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  16. Catecholamine stress alters neutrophil trafficking and impairs wound healing by beta2-adrenergic receptor-mediated upregulation of IL-6.
    Authors: Kim M, Gorouhi F, Ramirez S, Granick J, Byrne B, Soulika A, Simon S, Isseroff R
    J Invest Dermatol, 2014;134(3):809-17.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  17. Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-alpha, and IL-1beta.
    Authors: Zhao Y, Xiao X, Frank S, Lin H, Xia Y
    Am J Physiol Endocrinol Metab, 2014;307(2):E186-98.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  18. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
    Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
    Nat Med, 2014;20(4):377-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  19. Maternal immune activation during gestation interacts with Disc1 point mutation to exacerbate schizophrenia-related behaviors in mice.
    Authors: Lipina T, Zai C, Hlousek D, Roder J, Wong A
    J Neurosci, 2013;33(18):7654-66.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo injection
  20. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
    Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
    J Immunol, 2013;190(10):5178-86.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Capture
  21. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  22. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
    Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
    Infect. Immun., 2012;80(1):410-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  23. CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.
    Authors: Roy M, Richard JF, Dumas A, Vallieres L
    J Neuroinflammation, 2012;9(0):18.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  24. Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
    Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
    J. Exp. Med., 2011;208(3):491-503.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  25. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009;183(9):5799-806.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  26. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009;11(4):524-30.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  27. Stratification is the key: inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis.
    Authors: Osuchowski MF, Connett J, Welch K, Granger J, Remick DG
    Crit. Care Med., 2009;37(5):1567-73.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  28. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008;125(2):272-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  29. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008;112(2):362-73.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  30. Maternal immune activation alters fetal brain development through interleukin-6.
    Authors: Smith SE, Li J, Garbett K, Mirnics K, Patterson PH
    J. Neurosci., 2007;27(40):10695-702.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  31. Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
    Authors: Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
    Bone, 2007;40(5):1276-83.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  32. Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
    Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
    J Ethnopharmacol, 2006;106(3):418-24.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  33. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  34. A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model.
    Authors: Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M
    J. Mol. Cell. Cardiol., 2006;41(5):885-92.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  35. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  36. Role of interleukin-6 in cardiomyocyte/cardiac fibroblast interactions during myocyte hypertrophy and fibroblast proliferation.
    Authors: Fredj S, Bescond J, Louault C, Delwail A, Potreau D
    J. Cell. Physiol., 2005;204(2):428-36.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  37. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2004;231(1):103-11.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  38. Endogenous IL-17 as a mediator of neutrophil recruitment caused by endotoxin exposure in mouse airways.
    Authors: Miyamoto M, Prause O, Sjostrand M, Laan M, Lotvall J, Linden A
    J. Immunol., 2003;170(9):4665-72.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  39. Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes.
    Authors: Pechova J, Stĕpánová G, Kovar L, Kopecky J
    Folia Parasitol., 2002;49(2):153-9.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Expand to show all 39 Citations
Isotype Controls
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Rat IgG1 Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG1 Isotype Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 4 (Based on 1 review)

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ELISA Mouse IL-6 Antibody MAB406
ELISA: Mouse IL-6 Antibody [MAB406]

Summary

ApplicationELISA
Sample TestedHuman recombinant antibody
SpeciesMouse

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