Mouse IL-6 Antibody (MP5-20F3)

R&D Systems | Catalog # MAB406

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry, Bioassay, Blocking, ELISA Capture, ELISA Development, ELISA Development (Capture), In vivo assay, Functional Assay, Isotype Control, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # MP5-20F3
View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse IL-6

Specificity

Detects mouse IL-6 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL‑6, recombinant porcine IL‑6, recombinant rat IL-6, rhIL-11, rhCT-1, or rhCLC is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IL-6 Antibody (MP5-20F3)

Detection of Recombinant Mouse IL-6 antibody by Western Blot.

Detection of Recombinant Mouse IL‑6 by Western Blot.

Western blot shows 25 ng of Recombinant Mouse IL-6 (406-ML), Recombinant Human IL-6 (206-IL) and Recombinant Rat IL-6 (506-RL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-6 Monoclonal Antibody (Catalog # MAB406) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for IL-6 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody.

Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody.

Recombinant Mouse IL-6 (406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-6 Monoclonal Antibody (Catalog # MAB406). The ND50 is typically 0.005-0.025 µg/mL.
Detection of IL-6 by Western Blot

Detection of IL-6 by Western Blot

Role of ZFP36 in intestinal ischemia–reperfusion (I/R)-induced acute lung injury.C57BL/6 mice were subjected to 60 min of intestinal ischemia followed by 0, 30, 60, and 90 min of reperfusion as indicated. Sham mice were included as the control. A ZFP36 mRNA and protein expression in lung tissues were analyzed by RT-qPCR and Western blot (n = 6 per group, **P < 0.01). B Immunohistochemical staining of lung tissues for ZFP36 of sham and I/R 60 min. Scale bars: 50 μm. C–F Levels of IL-1 beta (C), TNF-alpha (D), and IL-6 (E) were measured by ELISA and Western blotting (F). G H&E staining of lung tissues. Red arrows outline collapsed alveoli, blue arrows outline multiple inflammatory cells infilitration, black arrows outline bronchial hemorrhage. Scale bars: 50 μm. H–J Arterial blood PaO2 (H), lung water content (I), and BALF protein content (J) were measured. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34238924), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-6 by Immunocytochemistry/ Immunofluorescence

Detection of IL-6 by Immunocytochemistry/ Immunofluorescence

Increased expression of IL-6 in primary cultured osteoblasts and chondrocytes from MLII mice. (a) Signal log ratio (SLR) of differentially expressed genes (SLR ≥ 2 or ≤ -2) in terminally differentiated osteoblasts from wild-type (WT) and MLII mice related to the gene ontology (GO) "Bone biological processes": "Bone mineralization" (GO 0030282) and "Osteoclast differentiation" (GO 0030316). (b) IL-6 immunostaining (green) of WT and MLII osteoblasts. Nuclei were visualized by 4′,6-diamidino-2-phenylindole (DAPI) staining (blue). Scale bar: 10 μm. (c) Concentration of IL-6 in conditioned media from WT and MLII osteoblasts (n = 5, mean ± SD, ***p ≤ 0.001). (d) Expression levels of Il6 mRNA related to Gapdh in primary osteoblasts, chondrocytes and osteoclasts from WT and MLII mice (n = 3, mean ± SD, ***p ≤ 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33574442), licensed under a CC-BY license. Not internally tested by R&D Systems.
Mouse IL-6 ELISA Standard Curve

Mouse IL-6 ELISA Standard Curve

Recombinant Mouse IL‑6 (Catalog # 406-ML) was serially diluted and captured by Rat Anti-Mouse IL‑6 Monoclonal Antibody (Catalog # MAB406) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of IL-6 by Western Blot

Detection of IL-6 by Western Blot

Role of ZFP36 in intestinal ischemia–reperfusion (I/R)-induced acute lung injury.C57BL/6 mice were subjected to 60 min of intestinal ischemia followed by 0, 30, 60, and 90 min of reperfusion as indicated. Sham mice were included as the control. A ZFP36 mRNA and protein expression in lung tissues were analyzed by RT-qPCR and Western blot (n = 6 per group, **P < 0.01). B Immunohistochemical staining of lung tissues for ZFP36 of sham and I/R 60 min. Scale bars: 50 μm. C–F Levels of IL-1 beta (C), TNF-alpha (D), and IL-6 (E) were measured by ELISA and Western blotting (F). G H&E staining of lung tissues. Red arrows outline collapsed alveoli, blue arrows outline multiple inflammatory cells infilitration, black arrows outline bronchial hemorrhage. Scale bars: 50 μm. H–J Arterial blood PaO2 (H), lung water content (I), and BALF protein content (J) were measured. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34238924), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-6 by Immunocytochemistry/ Immunofluorescence

Detection of IL-6 by Immunocytochemistry/ Immunofluorescence

Increased expression of IL-6 in primary cultured osteoblasts and chondrocytes from MLII mice. (a) Signal log ratio (SLR) of differentially expressed genes (SLR ≥ 2 or ≤ -2) in terminally differentiated osteoblasts from wild-type (WT) and MLII mice related to the gene ontology (GO) "Bone biological processes": "Bone mineralization" (GO 0030282) and "Osteoclast differentiation" (GO 0030316). (b) IL-6 immunostaining (green) of WT and MLII osteoblasts. Nuclei were visualized by 4′,6-diamidino-2-phenylindole (DAPI) staining (blue). Scale bar: 10 μm. (c) Concentration of IL-6 in conditioned media from WT and MLII osteoblasts (n = 5, mean ± SD, ***p ≤ 0.001). (d) Expression levels of Il6 mRNA related to Gapdh in primary osteoblasts, chondrocytes and osteoclasts from WT and MLII mice (n = 3, mean ± SD, ***p ≤ 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33574442), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-6 by Western Blot

Detection of IL-6 by Western Blot

IL‐6 released from activated microglia induces the apoptosis of astrocytes in vitro. A) Representative western blot images of Bax, Bcl‐2 and cleaved Caspase‐3 in astrocytes treated with PBS, IL‐6 and LPS. B) Western blot analysis of Bax (n = 7), Bcl‐2 (n = 7) and cleaved Caspase‐3 (n = 4) expression of astrocytes treated with PBS, IL‐6 and LPS. One‐way ANOVA. C) Representative images of FACS analysis of apoptosis in astrocytes treated with PBS (Con) or IL‐6. D) Apoptosis rate of astrocytes treated with PBS (Con) or IL‐6. Unpaired t test. n = 5. E) Drawing of primary cultured astrocytes stimulated with BzATP‐treated microglia conditioned medium (MCM). F) Representative images of FACS analysis of astrocytes treated with PBS (Control), BzATP, MCM alone or MCM combined with IL‐6 antibody. G) Apoptosis rate of astrocytes treated with Con, BzATP, MCM alone or MCM combined with IL‐6 antibody. One‐way ANOVA. n = 6. All data are shown as mean ± S.E.M, * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39888279), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-6 by Immunohistochemistry

Detection of IL-6 by Immunohistochemistry

IL‐6 signaling deficiency reduces alopecia and radiodermatitis in irradiated miceARelative expression and co‐localization of IL6 mRNA and IL‐6 Signaling Scoring in integrated skin cells by blended UMAPs.BQuantification by real‐time qPCR analysis of Il6 mRNA in the skin of naïve and irradiated (15 Gy) WT mice at indicated times, (n = 3–6).CQuantification of ventral hair loss area 21 days post‐IR (15 Gy) in WT and IL‐6−/− mice, (n = 11).DPhotographic images showing hair loss (area demarked by yellow dashed line) 21 days post‐IR (15 Gy) in wild type (WT) and IL‐6−/− mice.EHistochemical (H&E) and immunostaining (red) showing skin morphology, and T‐cell (CD3+) and neutrophil (Ly6b+) infiltration in WT or IL‐6−/− mice before (naïve), and 14 days (H&E, Ly6b+), or 21 days (CD3+) post‐IR. Infiltrating CD3+ T cells (yellow arrows) within degenerating hair follicles (HF; dashed lines) are indicated. Scale bars, 100 μm.FQuantification of epidermal thickness at 14 days post‐IR (n = 5–8).GKinetics of neutrophil (Ly6b+; n = 3–9) and T‐cell (CD3+; n = 3) infiltration.HQuantification by real‐time qPCR analysis of Tnfa and Ccl3 mRNAs in whole skin of WT and IL‐6−/− mice at indicated times post‐IR (15 Gy), (n = 5–7).Data information: Data represent mean ± SD (B, C, F, H) or mean ± SEM (G). *P < 0.05, **P < 0.01, **P < 0.01, ****P < 0.001 by one‐way ANOVA with Dunnett's multiple comparison test (B), two‐tailed Mann–Whitney test (C, F, H), or two‐way ANOVA with Bonferroni's post hoc multiple comparisons test (G); five independent experiments (See also Fig EV3 and Appendix Figs S7 and S9).Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.15252/emmm.202115653), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-6 by Immunocytochemistry/ Immunofluorescence

Detection of IL-6 by Immunocytochemistry/ Immunofluorescence

Effects of NF-kappa B or IL-6 inhibition on corneal re‐epithelialization and nerve regeneration in LGE-treated mice. (A) Representative fluorescein sodium staining images of corneal epithelial wounds at multiple time points (0–42 hours) after abrasion in control mice, and LGE‐treated mice administered PBS, CAPE, isotype IgG, or anti‐IL‐6 antibody. (B, C) Quantification of residual corneal wound area over time in each group of mice. (B) Comparison among control, LGE + PBS, and LGE + CAPE groups. (C) Comparison among control, LGE + isotype IgG, and LGE + anti‐IL‐6 antibody groups. (D, E) Quantification of dividing basal epithelial cells in each group of mice. (D) Comparison among control, LGE + PBS, and LGE + CAPE groups; (E) Comparison among control, LGE + isotype IgG, and LGE + anti‐IL‐6 antibody groups. (F) Immunofluorescence images of whole-mount corneal nerves at 6 days post‐injury using anti‐ beta ‐III tubulin antibody conjugated with NL557. Upper panels show full corneal view (scale bars = 500 μm); lower panels show magnified central region (scale bars = 200 μm). (G, H) Quantification of corneal nerve fiber density (G) and mechanical sensitivity (H) in control, LGE + PBS, and LGE + CAPE groups. (I, J) Quantification of corneal nerve fiber density (I) and mechanical sensitivity (J) in control, LGE + isotype IgG, and LGE + anti‐IL‐6 antibody groups. All values are presented as mean ± SD. Sample size: n = 6 mice in each group (B–E, G–J). Statistical significance: *P < 0.05, **P < 0.01, and ***P < 0.001 for LGE‐treated mice versus control mice; #P < 0.05, ##P < 0.01, and ###P < 0.001 for LGE + CAPE or anti‐IL‐6 antibody versus respective vehicle (PBS or IgG); +P < 0.05, ++P < 0.01, and +++P < 0.001 for LGE + CAPE or anti‐IL‐6 antibody versus control. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fimmu.2025.1684290/full), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-6 by Immunohistochemistry

Detection of IL-6 by Immunohistochemistry

(Related to Fig 2). IL‐6 signaling blockade reduces hair loss and dermatitis in irradiated miceAPhotographic images depicting the kinetics of radiodermatitis and hair loss in ventral views of representative wild‐type mice irradiated (15 Gy) to the head and neck. Times (in days) post‐IR are indicated. Serous exudates appear under the chin at 8 days post‐IR with desquamation and alopecia beginning at about 14 days post‐IR.BPhotographic images showing ventral alopecia in wild‐type mice treated with neutralizing IL‐6 mAb (Anti‐IL‐6) or control mAb (IgG; 200 μg, i.p) prior to and 8 days following irradiation (14 Gy) to the head and neck. Two independent experiments.CQuantification of the area of hair loss of mice in (B) at 14 days post‐IR, (n = 4–5). Data are individual mice. Two independent experiments.DHistochemical (H&E) and immunostaining of skin thin sections from mice in (B) showing morphology and neutrophil (Ly6b) infiltration (red staining). Scale bar, 50 μm.EQuantification of epidermal thickness in specimens from (D), (n = 4–5).FPhotographic images showing ventral alopecia in wild‐type mice (WT) and sgp130Fc transgenic mice irradiated (14 Gy) to the head and neck 21 days post‐IR. Five independent experiments.GQuantification of the area of hair loss of mice in (F), (n = 4).HHistochemical (H&E) staining of skin thin sections from mice in (F) and quantification of the epidermal thickness (right), (n = 4–7). Scale bar, 100 μm.Data information: Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by two‐tailed Student's t‐test (E), or Mann–Whitney test (G, H).Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.15252/emmm.202115653), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IL-6 Antibody (MP5-20F3)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑6 (Catalog # 406-ML)

Neutralization

Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND50) is typically 0.005-0.025 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6.

Mouse IL-6 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse IL‑6 Biotinylated Antibody (Catalog # BAF406)
  • Standard: Recombinant Mouse IL-6 Protein (Catalog # 406-ML)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 6 reviews rated 4.7 using MAB406 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature mouse IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Mouse IL-6 shares 39% and 85% aa sequence identity with human and rat IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for Mouse IL-6 Antibody (MP5-20F3)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-6 Antibody (MP5-20F3)

For research use only

Citations for Mouse IL-6 Antibody (MP5-20F3)

Customer Reviews for Mouse IL-6 Antibody (MP5-20F3) (6)

4.7 out of 5
6 Customer Ratings
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Showing  1 - 5 of 6 reviews Showing All
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  • IL-6 in mouse samples
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Lung tissue
    Verified Customer | Posted 06/03/2025
    IL-6 expression in WT animals
    1:20 in antibody diluent 2. Incubation time - 60min.
    Mouse IL-6 Antibody (MP5-20F3) MAB406
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Mouse IL-6 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Heart tissue
    Species: Mouse
    Verified Customer | Posted 08/12/2021
    Mouse IL-6 Antibody (MP5-20F3) MAB406
  • Mouse IL-6 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Heart tissue and Ear tissue
    Species: Mouse
    Verified Customer | Posted 02/10/2021
    Mouse IL-6 Antibody (MP5-20F3) MAB406
  • Name: Anonymous
    Application: Cell Invasion
    Sample Tested: Cancer-associated Fibroblasts and tumor cells
    Species: Mouse
    Verified Customer | Posted 04/11/2019
    Mouse IL-6 Antibody (MP5-20F3) MAB406
  • Name: Anonymous
    Application: Cell depletion
    Sample Tested: Pancreatic cancer cells
    Species: Mouse
    Verified Customer | Posted 05/11/2018
  • Mouse IL-6 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Human recombinant antibody
    Species: Mouse
    Verified Customer | Posted 12/08/2017
    Mouse IL-6 Antibody (MP5-20F3) MAB406

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