Mouse Integrin  beta 4/CD104 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Integrin beta 4/CD104 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Integin  beta 4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin  beta 4/CD104
    Asn29-Cys707
    Accession # NP_001005608
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Integrin  beta 4/CD104 by Western Blot. Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Integrin  beta 4/CD104 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4054) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Three specific bands were detected for Integrin  beta 4/CD104 at approximately 150-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 4/CD104

Integrin beta 4 (also GP150 and CD104) is a 150‑180 kDa member of the integrin beta family of molecules. It is found on keratinocytes, Schwann cells and perineural fibroblasts, and participates in the formation of epithelial hemidesmosomes by forming noncovalent heterodimers with Integrin alpha 6. Mature mouse Integrin beta 4 is a type I transmembrane glycoprotein that is 1774 amino acids (aa) in length. It contains a 683 aa extracellular domain (aa 29‑711) and a large 1068 aa cytoplasmic region. Three variant splice forms may exist. One shows a GlyGluHis insertion after Leu1103, a second shows a deletion of aa 1447‑1499, and a third shows the same deletion accompanied by an insertion of 65 aa after Thr1367. Over aa 29‑711, mouse beta 4 shares 88% and 96% aa identity with human and rat beta 4, respectively.

  • Entrez Gene IDs:
    3691 (Human); 192897 (Mouse); 25724 (Rat)
  • Alternate Names:
    CD104 antigen; CD104; GP150; Integrin beta 4; integrin beta-4 subunit; integrin beta-4; integrin, beta 4; ITGB4
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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