Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Integrin beta 4/CD104 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin  beta 4/CD104
Asn29-Cys707
Accession # NP_001005608

Specificity

Detects mouse Integrin beta 4/CD104 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Integin  beta 4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Integrin  beta 4/CD104 Antibody

Detection of Mouse Integrin beta 4/CD104 antibody by Western Blot.

Detection of Mouse Integrin beta 4/CD104 by Western Blot.

Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Integrin beta 4/CD104 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4054) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Three specific bands were detected for Integrin beta 4/CD104 at approximately 150-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse Integrin  beta 4/CD104 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse skin tissue

Reviewed Applications

Read 1 review rated 5 using AF4054 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Integrin beta 4/CD104

Integrin beta 4 (also GP150 and CD104) is a 150‑180 kDa member of the integrin beta family of molecules. It is found on keratinocytes, Schwann cells and perineural fibroblasts, and participates in the formation of epithelial hemidesmosomes by forming noncovalent heterodimers with Integrin alpha 6. Mature mouse Integrin beta 4 is a type I transmembrane glycoprotein that is 1774 amino acids (aa) in length. It contains a 683 aa extracellular domain (aa 29‑711) and a large 1068 aa cytoplasmic region. Three variant splice forms may exist. One shows a GlyGluHis insertion after Leu1103, a second shows a deletion of aa 1447‑1499, and a third shows the same deletion accompanied by an insertion of 65 aa after Thr1367. Over aa 29‑711, mouse beta 4 shares 88% and 96% aa identity with human and rat beta 4, respectively.

Alternate Names

CD104, ITGB4

Entrez Gene IDs

3691 (Human); 192897 (Mouse); 25724 (Rat)

Gene Symbol

ITGB4

UniProt

NP_001005608

Additional Integrin beta 4/CD104 Products

Product Documents for Mouse Integrin  beta 4/CD104 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Integrin  beta 4/CD104 Antibody

For research use only

Citations for Mouse Integrin  beta 4/CD104 Antibody

Customer Reviews for Mouse Integrin  beta 4/CD104 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse Integrin  beta 4/CD104 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Mouse Integrin  beta 4/CD104 Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Verified Customer | Posted 12/20/2023
    Western Blot: whole cell lysates from MDA-MB-231, SUM159 and MCF-7 cells were loaded with 50 ug/lane. 10% SDS-PAGE. ITGB4 Antibody (AF4054) was used for primary antibody: 1:2000, 4℃, overnight.
    Mouse Integrin  beta 4/CD104 Antibody AF4054
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies