Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IRF1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IRF1
Thr147-Pro329
Accession # P15314

Specificity

Detects mouse IRF1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IRF1 Antibody

Detection of Mouse IRF1 antibody by Western Blot.

Detection of Mouse IRF1 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse IRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4715) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for IRF1 was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse IRF1 antibody by Western Blot.

Detection of Mouse IRF1 antibody by Western Blot.

Whole cell lysates from MCF7 and SUM159 cells were loaded with 50 ug/lane.10% SDS-PAGE. IRF1 Antibody (AF4715) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.

Applications for Mouse IRF1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 2 reviews rated 4.5 using AF4715 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IRF1

IRF1 is a member of the interferon regulator transcription factor family, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF1 knock out mice show defects in NK and T cell function, as well as, develop CD30+ lymphoproliferative disease.

Long Name

Interferon Regulatory Factor 1

Alternate Names

MAR

Entrez Gene IDs

3659 (Human); 16362 (Mouse); 24508 (Rat)

Gene Symbol

IRF1

UniProt

P15314

Additional IRF1 Products

Product Documents for Mouse IRF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse IRF1 Antibody

For research use only

Citations for Mouse IRF1 Antibody

Customer Reviews for Mouse IRF1 Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Mouse IRF1 Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: breast cancer MCF7 cells and SUM-159PT
    Species: Human
    Verified Customer | Posted 11/28/2024
    Western Blot: whole cell lysates from MCF7 and SUM159 cells were loaded with 50 ug/lane. 10% SDS-PAGE. IRF1 Antibody (AF4715) was used for primary antibody: 1:1000, 4℃, overnight.
    Mouse IRF1 Antibody AF4715
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22464096
    Species: Mouse
    Verified Customer | Posted 01/08/2015

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