Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IRF3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IRF3
Thr201-Ile419
Accession # P70671

Specificity

Detects mouse IRF3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IRF3 Antibody

Detection of Mouse IRF3 antibody by Western Blot.

Detection of Mouse IRF3 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse IRF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4454) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IRF3 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse IRF3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IRF3

IRF3 is a member of the interferon regulator transcription factor family. IRF3 is typically found inactive in the cytoplasm. IRF3 is activated by the toll like receptors (TLR3 and TLR4), as well as, viral entry. Upon activation and phosphorylation, IRF3 heterodimerizes with CREBBP, which translocates to the nucleus to stimulate transcription. IRF3 does not contain transactivation function on its own; it must either recruit or be part of a complex of transcription factors to stimulate gene transcription.

Long Name

Interferon Regulatory Factor 3

Alternate Names

interferon regulatory factor 3, IRF-3

Entrez Gene IDs

3661 (Human); 54131 (Mouse)

Gene Symbol

IRF3

UniProt

P70671

Additional IRF3 Products

Product Documents for Mouse IRF3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IRF3 Antibody

For research use only

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