Mouse IRF3 Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse IRF3 by Western Blot.
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Product Details
Supplemental Products

Mouse IRF3 Antibody Summary

Species Reactivity
Detects mouse IRF3 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse IRF3
Accession # P70671
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IRF3 antibody by Western Blot. View Larger

Detection of Mouse IRF3 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse IRF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4454) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IRF3 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF3

IRF3 is a member of the interferon regulator transcription factor family. IRF3 is typically found inactive in the cytoplasm. IRF3 is activated by the toll like receptors (TLR3 and TLR4), as well as, viral entry. Upon activation and phosphorylation, IRF3 heterodimerizes with CREBBP, which translocates to the nucleus to stimulate transcription. IRF3 does not contain transactivation function on its own; it must either recruit or be part of a complex of transcription factors to stimulate gene transcription.

Long Name
Interferon Regulatory Factor 3
Entrez Gene IDs
3661 (Human); 54131 (Mouse)
Alternate Names
interferon regulatory factor 3; IRF3; IRF-3

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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