Mouse LAIR1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10092-100
MAB10092-SP
Detection  of Mouse LAIR1 by Western Blot.
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Mouse LAIR1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LAIR1 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2459C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line, NS0-derived mouse LAIR1
Gln22-Tyr141 & Ser25-Tyr141
Accession # Q8BG84
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Mouse LAIR1 by Western Blot. View Larger

Detection of Mouse LAIR1 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and mouse thymus tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Mouse LAIR1 Monoclonal Antibody (Catalog # MAB10092) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for LAIR1 at approximately 36-14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1) is an inhibitory receptor of the Ig superfamily that is structurally related to inhibitory members of KIR and ILT/CD85 families (1-3). It is expressed on immune cells, including NK cells, T cells, B cells, monocytes, immature neutrophils, dendritic cells and most thymocytes (2-4). The 253 amino acid (aa) type I transmembrane (TM) protein contains a 21 aa signal sequence, a 124 aa extracellular domain (ECD), a 20 aa TM domain and a 98 aa cytoplasmic domain. The ECD includes one C2-type Ig-like domain and two potential N-linked glycosylation sites. Tyrosine phosphorylation of two cytoplasmic ITIM motifs results in recruitment of phosphatases and down-regulation of signaling through activating receptors (2, 3, 5). Crosslinking of LAIR1 inhibits processes such as B cell receptor-mediated activation, NK cell and T cell cytotoxicity and basophil degranulation (1-3). Four mouse LAIR1 splice variants have been identified, but it is not known whether they are expressed as proteins (3). LAIR1b, which is the major alternate transcript, lacks most of the ECD. Of the minor transcripts, LAIR1c lacks a signal sequence, and LAIR1d and 1e lack a TM sequence. All mouse splice forms are identical in the last 90 aa of the cytoplasmic domain. LAIR1 shows high‑affinity binding of collagens that results in inhibition of degranulation in a basophilic leukemia cell line (6). Human and mouse LAIR1 ECD share only 32% aa identity but, where studied, sites of expression and activity are similar (3-6). Mouse LAIR1 ECD also shares 62%, 31% and 28% aa identity with rat, canine, and bovine orthologs, respectively.

References
  1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
  2. Meyaard, L. et al. (1997) Immunity 7:283.
  3. Lebbink, R.J. et al. (2004) J. Immunol. 172:5535.
  4. Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828.
  5. Verbrugge, A. et al. (2003) Int. Immunol. 15:1349.
  6. Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419.
Long Name
Leukocyte-associated Ig-like Receptor 1
Entrez Gene IDs
3903 (Human); 52855 (Mouse); 574531 (Rat)
Alternate Names
CD305 antigen; CD305; CD305leukocyte-associated Ig-like receptor 1; hLAIR1; LAIR1; LAIR-1; leukocyte-associated immunoglobulin-like receptor 1

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