Mouse Leptin R Antibody Summary
Accession # P48356
Mouse Leptin R Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Leptin R in HEK293 Human Cell Line transfected with Mouse Leptin R and EGFP by Flow Cytometry. HEK293 human cell line transfected with Mouse Leptin R and EGFP was stained with (A) Rat Anti-Mouse Leptin R Biotinylated Monoclonal Antibody (Catalog # MAB497) or (B) isotype control antibody (MAB006), followed by Allophycocyanin-conjugated anti-Rat IgG Secondary Antibody (F0113). Staining was performed using our Staining Membrane-associated Proteins protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leptin R
Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R is encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype. Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R binds leptin with high affinity and is a potent leptin antagonist.
- Tartaglia, L.A. et al. (1995) Cell 83:1263.
- Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
- Lee, J. I. and J.M. Friedman (1996) Nature 379:632.
- Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
- Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.
Citation for Mouse Leptin R Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
High fat diet and leptin promote tumor progression by inducing myeloid-derived suppressor cells
Authors: VK Clements, T Long, R Long, C Figley, DMC Smith, S Ostrand-Ro
J. Leukoc. Biol., 2018;0(0):.
Sample Types: In Vivo
Applications: In Vivo
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