Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MAGP-1/MFAP2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MAGP-1/MFAP2
Gln17-Cys183
Accession # P55002

Specificity

Detects mouse MAGP-1/MFAP2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human MAGP-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse MAGP‑1/MFAP2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse MAGP-1/MFAP2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MAGP-1/MFAP2

MAGP-1 (Microfibril-associated glycoprotein 1; also MFAP-2) is a 30‑33 kDa member of the MFAP family of proteins. It is secreted by endothelial and smooth muscle cells. MAGP-1 apparently mediates the release of Notch extracellular domain and also likely binds to type IV collagen and contributes to elastin fiber formation. Mature mouse MAGP-1 is 167 amino acids (aa) in length. It contains a transglutamination site at Gln20, a tyrosine sulfation motif (aa 46‑49), two potential O-linked glycosylation sites (Thr56 and Thr76), and a Cys-rich C-terminus that generates intermolecular disulfide bonds. One potential splice variant exists that is strictly intracellular and a second shows a 22 aa substitution for the C-terminal 127 amino acids. Mature mouse MAGP-1 is 94% and 87% aa identical to rat and human MAGP‑1, respectively.

Long Name

Microfibril-associated Glycoprotein 1

Alternate Names

MAGP1, MFAP2

Entrez Gene IDs

4237 (Human); 17150 (Mouse); 313662 (Rat)

Gene Symbol

MFAP2

UniProt

P55002

Additional MAGP-1/MFAP2 Products

Product Documents for Mouse MAGP‑1/MFAP2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse MAGP‑1/MFAP2 Antibody

For research use only

Citations for Mouse MAGP‑1/MFAP2 Antibody

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