Mouse MMP-12 Antibody

R&D Systems | Catalog # AF3467

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MMP-12 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMP-12
Ala18-Cys462
Accession # EDL24933

Specificity

Detects mouse MMP-12 in direct ELISAs and Western blots. In direct ELISAs,less than 20% cross-reactivity with recombinant human MMP‑12 is observed, and less than 5% cross-reactivity with recombinant mouse (rm) MMP-3, rmMMP-8, and rmMMP-10 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse MMP-12 Antibody

Detection of Mouse MMP‑12 antibody by Western Blot.

Detection of Mouse MMP‑12 by Western Blot.

Western blot shows mouse serum and mouse plasma. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse MMP-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-12 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse MMP-12 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse MMP‑12 (Catalog # 3467‑MP), see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Mouse serum and mouse plasma

Reviewed Applications

Read 1 review rated 5 using AF3467 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-12

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-12 (macrophage elastase) is found in macrophages and its expression in monocytes can be induced by cytokines such as GM-CSF and CD40 signaling. In addition to elastin, MMP-12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha 1-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3. MMP-12 is required for macrophage-mediated proteolysis and matrix invasion in mice. MMP-12 is proposed to have a direct role in the pathogenesis of aortic aneurysms and in the development of pulmonary emphysema that results from chronic inhalation of cigarette smoke. Structurally, the pro-MMP-12 consists of following domains: a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain. The recombinant mouse MMP-12 corresponds to the pro form that can be activated.

Long Name

Matrix Metalloproteinase 12

Alternate Names

MMP12

Entrez Gene IDs

4321 (Human); 17381 (Mouse)

Gene Symbol

MMP12

UniProt

EDL24933

Additional MMP-12 Products

Product Documents for Mouse MMP-12 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MMP-12 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Mouse MMP-12 Antibody

Customer Reviews for Mouse MMP-12 Antibody (1)

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Customer Images

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  • Mouse MMP-12 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 10X concentration of conditioned media from primary glia cell culture
    Species: Mouse
    Verified Customer | Posted 07/29/2016
    Conditioned media was serum free and concentrated 10X. Lane1: molecular weight 50kD-75kD. Lane2: mmp12 knockout sample. Lane3: mmp12 wildtype sample. Block with 4% milk in TBST for 30min. 1' antibody 1:1000 in 4%BSA in TBST overnight. 2' HRP conjugated antibody against Goat 1:5000 in milk for 1h in RT. ECL detection 1min.
    Mouse MMP-12 Antibody AF3467

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