Mouse Nectin-3 Antibody

R&D Systems | Catalog # AF3498

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Hamster

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Nectin-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Nectin-3
Leu56-Asp400
Accession # Q9JLB9.1

Specificity

Detects mouse Nectin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human Nectin-3 is observed and less than 2% cross-reactivity with recombinant mouse (rm) Nectin-1 and rmNectin-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Nectin-3 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Nectin‑3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nectin-3

Nectin-3, also known as poliovirus receptor-related 3, is a 95 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. The extracellular domains of the Nectin family form cis-homodimers that are homophilic and heterophilic trans-interacting cell adhesion molecules, which play important roles in various cell-cell junctions. Mouse Nectin-3 extracellular domain shares 96% and 94% amino acid sequence identity with that of rat and human Nectin-3, respectively.

Long Name

Poliovirus Receptor Related 3

Alternate Names

CD113, Nectin3, PPR3, PVRL3, PVRR3

Entrez Gene IDs

25945 (Human); 58998 (Mouse); 288124 (Rat)

Gene Symbol

NECTIN3

UniProt

Q9JLB9.1

Additional Nectin-3 Products

Product Documents for Mouse Nectin-3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Nectin-3 Antibody

For research use only

Citations for Mouse Nectin-3 Antibody

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