The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Rat
Applications
Validated:
Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 197643
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Product Specifications
Immunogen
E. coli-derived recombinant mouse p53
Leu28-Phe325
Accession # NP_035770
Leu28-Phe325
Accession # NP_035770
Specificity
Detects mouse p53 in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse p53 Antibody
Immunoprecipitation of Mouse p53.
Balb/3T3 mouse embryonic fibroblast cell line was untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 6 hours. p53 was immunoprecipitated from 500 µg of cell lysate following incubation with 1-3 µg Rat Anti-Mouse p53 Monoclonal Antibody (Catalog # MAB1746) for 1 hour on ice. p53-antibody complexes were absorbed using goat anti-rat IgG sepharose (Invitrogen). Immunoprecipitated p53 was detected by Western blot using a 1:5000 dilution of Human/Mouse/Rat p53 Horseradish Peroxidase-conjugated Antigen Affinity-purified Polyclonal Antibody (HAF1355). View our recommended buffer recipes for immunoprecipitation.Applications for Mouse p53 Antibody
Application
Recommended Usage
Immunoprecipitation
1-3 µg/500 µg cell lysate
Sample: Balb/3T3 mouse embryonic fibroblast cell line treated with camptothecin, see our available Western blot detection antibodies
Sample: Balb/3T3 mouse embryonic fibroblast cell line treated with camptothecin, see our available Western blot detection antibodies
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: p53
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
UniProt
Additional p53 Products
Product Documents for Mouse p53 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse p53 Antibody
For research use only
Citations for Mouse p53 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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Associated Pathways
Apoptosis Signaling Pathway
MAPK Signaling: Oxidative Stress Pathway
mTOR Signaling Pathway
