hh Mouse p53 Antibody MAB1746: R&D Systems

Mouse p53 Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse p53 in Western blots.
  • Source
    Monoclonal Rat IgG2B Clone # 197643
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse p53
    Leu28-Phe325
    Accession # NP_035770
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunoprecipitation
    2 µg/500 µg cell lysate
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunoprecipitation
Immunoprecipitation of Mouse p53.

Balb/3T3 mouse embryonic fibroblast cell line was untreated
(-) or treated (+) with 1 μM camptothecin (CPT) for 6 hours. p53 was immunoprecipitated from 500 μg of cell lysate following incubation with 1‑3 µg Rat Anti-Mouse p53 Monoclonal Antibody (Catalog # MAB1746) for 1 hour on ice. p53-antibody complexes were absorbed using goat anti-rat IgG sepharose (Invitrogen). Immunoprecipitated p53 was detected by Western blot using a 1:5000 dilution of Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355). View our recommended buffer recipes for immunoprecipitation.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

  • Entrez Gene IDs:
    7157 (Human); 22059 (Mouse); 24842 (Rat)
  • Alternate Names:
    Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Early-life nutrition influences thymic growth in male mice that may be related to the regulation of longevity.
    Authors: Chen JH, Tarry-Adkins JL, Heppolette CA, Palmer DB, Ozanne SE
    Clin. Sci., 2010;118(6):429-38.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Adverse effects of reduced oxygen tension on the proliferative capacity of rat kidney and insulin-secreting cell lines involve DNA damage and stress responses.
    Authors: Chen JH, Jones RH, Tarry-Adkins J, Smith NH, Ozanne SE
    Exp. Cell Res., 2008;314(16):3075-80.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
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Isotype Controls
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Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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