Mouse Park7/DJ-1 DuoSet ELISA

• Assay Type
  Solid Phase Sandwich ELISA
• Format
  96-well strip plate
• Sample Volume Required
  100 µL
• Sufficient Materials
  For five 96-well plates*
• Specificity
  Please see the product datasheet
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Ancillary Reagent Kit Available
DY008 , DuoSet ELISA Ancillary Reagent Kit 2 - Please Inquire
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Park7 / DJ-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.


Product Features
• Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
• Development protocols are provided to guide further assay optimization
• Assay can be customized to your specific needs
• Economical alternative to complete kits
Kit Content
• Capture Antibody
• Detection Antibody
• Recombinant Standard
• Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent


*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Product Datasheets
Product Datasheet


COA
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SDS
Preparation and Storage
• Stability & Storage
  Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Park7/DJ-1
Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson's disease.
• Long Name:
  Parkinson Disease 7
• Entrez Gene IDs:
  11315 (Human); 57320 (Mouse); 117287 (Rat)
• Alternate Names:
  DJ1; DJ-1; DJ1FLJ34360; EC 3.4; FLJ27376; FLJ92274; Oncogene DJ1; Park7; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; protein DJ-1

FAQs

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