Mouse Phospho-p53 (S18) Antibody

Name: Mouse Phospho-p53 (S18) Antibody
Brand: R&D Systems
SKU: AF2887

Catalog #: AF2887
1 Citation Datasheet / COA / SDS

Discontinued Product

AF2887 has been discontinued.

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Detection of Mouse Phospho-p53 (S18) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Phospho-p53 (S18) Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse p53 when phosphorylated at S18.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing mouse p53 S18 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Phospho-p53 (S18) by Western Blot. Western blot shows p53 immunoprecipitated from lysates of Balb/3T3 mouse embryonic fibroblast cell line using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355). Balb/3T3 cell line was untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 6 hours. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Mouse Phospho-p53 (S18) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2887) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S18) was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

The p53 tumor suppressor protein acts to enforce cell cycle checkpoints or signal apoptosis in cells that have incurred genotoxic damage. The ATM or ATR kinases phosphorylate mouse p53 at serine 18 in response to genotoxic stress. Serine 18 phosphorylation contributes to p53 stabilization and enhances transactivation of p53 target genes.

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Alternate Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse Phospho-p53 (S18) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1