|Detection of Mouse Phospho-p53 (S18) by Western Blot. Western blot shows p53 immunoprecipitated from lysates of Balb/3T3 mouse embryonic fibroblast cell line using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355). Balb/3T3 cell line was untreated (-) or treated (+) with 1 μM camptothecin (CPT) for 6 hours. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Mouse Phospho-p53 (S18) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2887) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S18) was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
The p53 tumor suppressor protein acts to enforce cell cycle checkpoints or signal apoptosis in cells that have incurred genotoxic damage. The ATM or ATR kinases phosphorylate mouse p53 at serine 18 in response to genotoxic stress. Serine 18 phosphorylation contributes to p53 stabilization and enhances transactivation of p53 target genes.
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