hh Mouse Rae-1 Pan Specific Antibody AF1136: R&D Systems

Mouse Rae-1 Pan Specific Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Rae‑1 gamma as well as mouse Rae-1 alpha, 1 beta, 1δ and 1 epsilon in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Rae‑1 gamma
    Leu29-Ser231
    Accession # O08604
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Rae‑1 gamma Fc Chimera (Catalog # 1136-RA)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 125 ng/mL of Recombinant Biotinylated Mouse NKG2D Fc Chimera to immobilized Recombinant Mouse Rae-1 gamma Fc Chimera (Catalog # 1136‑RA) coated at 1 µg/mL (100 µL/well). At 4 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Rae‑1 in Mouse Embryo. Rae‑1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Rae‑1 Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1136) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to axons of primary sensory neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rae-1

Rae-1 gamma is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, beta, δ and epsilon. Amino acid sequence identity within this family ranges from 88‑95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI‑linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10‑14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1‑6).

  • References:
    1. Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
    2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
    3. Cerwenka, A. et al. (2000) Immunity 12:721.
    4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
    5. Diefenbach, A. et al. (2001) Nature 413:165.
    6. NKG2D and its Ligands, www.RnDSystems.com.
  • Long Name:
    Retinoic Acid Early Transcript 1
  • Entrez Gene IDs:
    8480 (Human); 19368 (Mouse)
  • Alternate Names:
    Rae1; Rae-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. The immunoreceptor NKG2D promotes tumour growth in a model of hepatocellular carcinoma
    Authors: S Sheppard, J Guedes, A Mroz, AM Zavitsanou, H Kudo, SM Rothery, P Angelopoul, R Goldin, N Guerra
    Nat Commun, 2017;8(0):13930.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. NK1.1(-) CD4(+) NKG2D(+) T cells suppress DSS-induced colitis in mice through production of TGF-?
    Authors: X Qian, C Hu, S Han, Z Lin, W Xiao, Y Ding, Y Zhang, L Qian, X Jia, G Zhu, W Gong
    J. Cell. Mol. Med, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  3. NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
    Authors: Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D
    J Leukoc Biol, 2015;97(3):583-98.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  4. CD4(+) NKG2D(+) T cells induce NKG2D down-regulation in natural killer cells in CD86-RAE-1epsilon transgenic mice.
    Authors: Lin Z, Wang C, Xia H, Liu W, Xiao W, Qian L, Jia X, Ding Y, Ji M, Gong W
    Immunology, 2014;141(3):401-15.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Sustained CTL activation by murine pulmonary epithelial cells promotes the development of COPD-like disease.
    Authors: Borchers MT, Wesselkamper SC, Curull V, Ramirez-Sarmiento A, Sanchez-Font A, Garcia-Aymerich J, Coronell C, Lloreta J, Agusti AG, Gea J, Howington JA, Reed MF, Starnes SL, Harris NL, Vitucci M, Eppert BL, Motz GT, Fogel K, McGraw DW, Tichelaar JW, Orozco-Levi M
    J. Clin. Invest., 2009;119(3):636-49.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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