Mouse Rae-1 Pan Specific Antibody

Catalog # Availability Size / Price Qty
AF1136
AF1136-SP

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Rae‑1 in Mouse Embryo.
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Citations (10)
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Mouse Rae-1 Pan Specific Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Rae‑1 gamma as well as mouse Rae-1 alpha, 1 beta, 1δ and 1 epsilon in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Rae‑1 gamma
Leu29-Ser231
Accession # O08604
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Rae‑1 gamma Fc Chimera (Catalog # 1136-RA)
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 125 ng/mL of Recombinant Biotinylated Mouse NKG2D Fc Chimera to immobilized Recombinant Mouse Rae-1 gamma Fc Chimera (Catalog # 1136‑RA) coated at 1 µg/mL (100 µL/well). At 4 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Rae-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Rae‑1 in Mouse Embryo. Rae-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Rae-1 Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1136) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to axons of primary sensory neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Mouse Rae-1 by Western Blot View Larger

Detection of Mouse Rae-1 by Western Blot Sciatic Nerve Crush Increases RAE1 in Peripheral Nerve Axons(A) Schematic diagram of sciatic nerve crush injury site in relation to lumbar DRGs (L3, L4, and L5).(B) Raet1 mRNA expression in ipsilateral L3–5 DRG 3 and 7 days after surgery by qPCR. Two-way ANOVA: effect of injury, F(1,8) = 180.95, p < 0.0001; effect of time, F(1,8) = 8.04, p = 0.0220. Bonferroni post-test: 3 days, t = 7.507, ∗∗∗p < 0.001; 7 days, t = 11.52, ∗∗∗p < 0.001. n = 3 mice per surgery per time point.(C) In situ hybridization with Raet1 probe (red) in L4 DRG immunolabeled for NeuN (blue) 6 days after partial sciatic nerve crush injury. Scale bar, 50 μm.(D) Distribution of Raet1 in situ spots in L4 DRG ipsi and contralateral to sciatic crush. Kolmogorov-Smirnov (KS) test: ∗∗∗p < 0.0001, KS D-value 0.2297. n = 3 mice, n = 3 sections per ipsi and contralateral DRG per mouse.(E) (Top) Pan-RAE1 western blot of sciatic nerve tissue (40 μg protein loading) 3 days after sciatic nerve crush or sham surgery. Neuronal cadherin control is shown. (Bottom) Relative expression of RAE1 protein in ipsilateral versus contralateral sciatic nerves 3 and 7 days after sham or crush surgery. One-way ANOVA, 3 days: F(3,16) = 8.505, p = 0.0013. Bonferroni post-test: sham ipsi versus contra, t = 0.2025, ns p > 0.05; crush ipsi versus contra, t = 3.678, ∗p < 0.05; ipsi sham versus crush, t = 4.438, ∗∗p < 0.01. One-way ANOVA, 7 days: F(3,16) = 5.119, p = 0.0113. Bonferroni post-test: sham ipsi versus contra, t = 0.1438, ns p > 0.05; crush ipsi versus contra, t = 3.342, ∗p < 0.05; ipsi sham versus crush, t = 3.043, ∗p < 0.05 (n = 5 mice per surgery group per time point).(F) (Top) Pan-RAE1 western blot of DRG (L3–5) tissue (40 μg protein loading) 3 days after sciatic nerve crush or sham surgery. Neuronal cadherin control. (Bottom) Relative expression of RAE1 protein in ipsilateral versus contralateral DRG 3 and 7 days after sham or crush surgery. One-way ANOVA: 3 days, F(3,16) = 0.438, p = 0.7288; 7 days, F(3,20) = 0.053, p = 0.983 (n = 5–6 mice per surgery group per time point).(G) Composite images of RAE1 immunolabeling in full-length contralateral and ipsilateral sciatic nerves taken from three individual mice 3 days after tight sciatic nerve ligation. Arrow, ligation site; arrowhead, proximal to ligation.(H) Maximum projection images of RAE1 (green) co-localization with axonal marker beta -tubulin III (magenta) in sciatic nerve 3 days after tight ligation. Scale bars, 50 μm.See also Figures S3 and S4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30712871), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Rae-1 by Western Blot View Larger

Detection of Mouse Rae-1 by Western Blot Sciatic Nerve Crush Increases RAE1 in Peripheral Nerve Axons(A) Schematic diagram of sciatic nerve crush injury site in relation to lumbar DRGs (L3, L4, and L5).(B) Raet1 mRNA expression in ipsilateral L3–5 DRG 3 and 7 days after surgery by qPCR. Two-way ANOVA: effect of injury, F(1,8) = 180.95, p < 0.0001; effect of time, F(1,8) = 8.04, p = 0.0220. Bonferroni post-test: 3 days, t = 7.507, ∗∗∗p < 0.001; 7 days, t = 11.52, ∗∗∗p < 0.001. n = 3 mice per surgery per time point.(C) In situ hybridization with Raet1 probe (red) in L4 DRG immunolabeled for NeuN (blue) 6 days after partial sciatic nerve crush injury. Scale bar, 50 μm.(D) Distribution of Raet1 in situ spots in L4 DRG ipsi and contralateral to sciatic crush. Kolmogorov-Smirnov (KS) test: ∗∗∗p < 0.0001, KS D-value 0.2297. n = 3 mice, n = 3 sections per ipsi and contralateral DRG per mouse.(E) (Top) Pan-RAE1 western blot of sciatic nerve tissue (40 μg protein loading) 3 days after sciatic nerve crush or sham surgery. Neuronal cadherin control is shown. (Bottom) Relative expression of RAE1 protein in ipsilateral versus contralateral sciatic nerves 3 and 7 days after sham or crush surgery. One-way ANOVA, 3 days: F(3,16) = 8.505, p = 0.0013. Bonferroni post-test: sham ipsi versus contra, t = 0.2025, ns p > 0.05; crush ipsi versus contra, t = 3.678, ∗p < 0.05; ipsi sham versus crush, t = 4.438, ∗∗p < 0.01. One-way ANOVA, 7 days: F(3,16) = 5.119, p = 0.0113. Bonferroni post-test: sham ipsi versus contra, t = 0.1438, ns p > 0.05; crush ipsi versus contra, t = 3.342, ∗p < 0.05; ipsi sham versus crush, t = 3.043, ∗p < 0.05 (n = 5 mice per surgery group per time point).(F) (Top) Pan-RAE1 western blot of DRG (L3–5) tissue (40 μg protein loading) 3 days after sciatic nerve crush or sham surgery. Neuronal cadherin control. (Bottom) Relative expression of RAE1 protein in ipsilateral versus contralateral DRG 3 and 7 days after sham or crush surgery. One-way ANOVA: 3 days, F(3,16) = 0.438, p = 0.7288; 7 days, F(3,20) = 0.053, p = 0.983 (n = 5–6 mice per surgery group per time point).(G) Composite images of RAE1 immunolabeling in full-length contralateral and ipsilateral sciatic nerves taken from three individual mice 3 days after tight sciatic nerve ligation. Arrow, ligation site; arrowhead, proximal to ligation.(H) Maximum projection images of RAE1 (green) co-localization with axonal marker beta -tubulin III (magenta) in sciatic nerve 3 days after tight ligation. Scale bars, 50 μm.See also Figures S3 and S4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30712871), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rae-1

Rae-1 gamma is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, beta, δ and epsilon. Amino acid sequence identity within this family ranges from 88‑95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI‑linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10‑14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1‑6).

References
  1. Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
  2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
  3. Cerwenka, A. et al. (2000) Immunity 12:721.
  4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  5. Diefenbach, A. et al. (2001) Nature 413:165.
  6. NKG2D and its Ligands, www.RnDSystems.com.
Long Name
Retinoic Acid Early Transcript 1
Entrez Gene IDs
8480 (Human); 19368 (Mouse)
Alternate Names
dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1; Rae1; Rae-1

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Citations for Mouse Rae-1 Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Crosstalk between cytotoxic CD8+ T cells and stressed cardiomyocytes triggers development of interstitial cardiac fibrosis in hypertensive mouse hearts
    Authors: Kurt Brassington, Peter Kanellakis, Anh Cao, Ban-Hock Toh, Karlheinz Peter, Alex Bobik et al.
    Frontiers in Immunology
  2. NKG2D signaling regulates IL-17A-producing gamma δT cells in mice to promote cancer progression
    Authors: Sophie Curio, Sarah C Edwards, Toshiyasu Suzuki, Jenny McGovern, Chiara Triulzi, Nagisa Yoshida et al.
    Discovery Immunology
  3. Effects of obesity on NK cells in a mouse model of postmenopausal breast cancer
    Authors: J Spielmann, L Mattheis, JS Jung, H Rau beta e, M Gla beta, I Bähr, D Quandt, J Oswald, H Kielstein
    Sci Rep, 2020-11-26;10(1):20606.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Toxicity Induced by a Bispecific T Cell-Redirecting Protein Is Mediated by Both T Cells and Myeloid Cells in Immunocompetent Mice
    Authors: C Godbersen-, TA Coupet, Z Grada, SC Zhang, CL Sentman
    J. Immunol., 2020-04-15;204(11):2973-2983.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Cytotoxicity of natural killer cells activated through NKG2D contributes to the development of bronchiolitis obliterans in a murine heterotopic tracheal transplant model
    Authors: T Kawakami, K Ito, Y Matsuda, M Noda, A Sakurada, Y Hoshikawa, Y Okada, K Ogasawara
    Am. J. Transplant, 2017-04-21;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. NK1.1(-) CD4(+) NKG2D(+) T cells suppress DSS-induced colitis in mice through production of TGF-?
    Authors: X Qian, C Hu, S Han, Z Lin, W Xiao, Y Ding, Y Zhang, L Qian, X Jia, G Zhu, W Gong
    J. Cell. Mol. Med, 2017-02-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. The immunoreceptor NKG2D promotes tumour growth in a model of hepatocellular carcinoma
    Authors: S Sheppard, J Guedes, A Mroz, AM Zavitsanou, H Kudo, SM Rothery, P Angelopoul, R Goldin, N Guerra
    Nat Commun, 2017-01-27;8(0):13930.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
    Authors: Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D
    J Leukoc Biol, 2015-01-12;97(3):583-98.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC, IHC-P
  9. CD4(+) NKG2D(+) T cells induce NKG2D down-regulation in natural killer cells in CD86-RAE-1epsilon transgenic mice.
    Authors: Lin Z, Wang C, Xia H, Liu W, Xiao W, Qian L, Jia X, Ding Y, Ji M, Gong W
    Immunology, 2014-03-01;141(3):401-15.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  10. Sustained CTL activation by murine pulmonary epithelial cells promotes the development of COPD-like disease.
    Authors: Borchers MT, Wesselkamper SC, Curull V, Ramirez-Sarmiento A, Sanchez-Font A, Garcia-Aymerich J, Coronell C, Lloreta J, Agusti AG, Gea J, Howington JA, Reed MF, Starnes SL, Harris NL, Vitucci M, Eppert BL, Motz GT, Fogel K, McGraw DW, Tichelaar JW, Orozco-Levi M
    J. Clin. Invest., 2009-02-09;119(3):636-49.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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