Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Human, Mouse, Rat, Hamster, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry, Simple Western, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Proprotein Convertase 9/PCSK9 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Proprotein Convertase 9
Ser156-Gln694
Accession # Q80W65

Specificity

Detects mouse and rat Proprotein Convertase 9/PCSK9 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody

Detection of Mouse and Rat Proprotein Convertase 9/PCSK9 antibody by Western Blot.

Detection of Mouse and Rat Proprotein Convertase 9/PCSK9 by Western Blot.

Western blot shows lysates of mouse liver tissue and rat embryonic cortical neuron. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Proprotein Convertase 9/PCSK9 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
Detection of Proprotein Convertase 9/PCSK9 by Western Blot

Detection of Proprotein Convertase 9/PCSK9 by Western Blot

AAV-delivery of NmeCas9 for in vivo genome editing. a Experimental outline of AAV8-sgRNA-hNmeCas9 vector tail-vein injections to target Pcsk9 (sgPcsk9) and Rosa26 (sgRosa26) in C57Bl/6 mice. Mice were sacrificed at 14 (n = 1) or 50 days (n = 5) post injection and liver tissues were harvested. Blood sera were collected at days 0, 25, and 50 post injection for cholesterol level measurement. b Serum cholesterol levels. p values are calculated by unpaired t test. cStacked histogram showing the percentage distribution of indels at Pcsk9 or Rosa26 in livers of mice, as measured by targeted deep-sequencing analyses. Data are presented as mean ± SD from five mice per cohort. d A representative anti-PCSK9 western blot using total protein collected from day 50 mouse liver homogenates. A total of 2 ng of recombinant mouse PCSK9 (r-PCSK9) was included as a mobility standard. The asterisk indicates a cross-reacting protein that is larger than the control recombinant protein Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30231914), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Proprotein Convertase 9/PCSK9 by Western Blot

Detection of Proprotein Convertase 9/PCSK9 by Western Blot

Infection of cardiomyocytes with human PCSK9. Serum-free cultured adult rat ventricular cardiomyocytes were infected with hPCSK9. Infection of cardiomyocytes with LacZ served as control. After 24 h, load free cell shortening was measured (cells paced at 2 Hz). a Representative immunoblot indicating the successful transfection of cardiomyocytes with hPCSK9. b Quantification of Western Blot shown in (a). c Load free cell-shortening expressed as delta L/L (%), (d) contraction velocity (µm/s) (e) and relaxation velocity (µm/s) of 89 (LacZ) and 90 (PCSK9) cells (10 independent experiments with intraassay variability p > 0.05). Statistical analysis was performed by Mann–Whitney test. *p ≤ 0.05. Data are mean ± SD Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33169229), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Proprotein Convertase 9/PCSK9 by Western Blot

Detection of Proprotein Convertase 9/PCSK9 by Western Blot

Fishmeal feeding leads to circulating PCSK9. Expression of hepatic genes important for steroid hormone synthesis and cholesterol metabolism in mice fed fishmeal, salmon filet or beef (n = 10). a Validation RNA sequencing analysis with RT-qPCR analysis of selected genes identified as low expressed in fishmeal compared to salmon or beef (Hmgcr, Pcsk9, Fabp5, Fdsp, Chrna4). b Expression of mRNAs encoding genes regulating cholesterol synthesis (Srebf2) and lipogenesis (Srebf1c and Mlxipl/Chrebp). c Expression of cholesterol metabolite sensors (Nr1h3/LXR alpha and Nr1h2/LXR beta ), cholesterol efflux transporters (Abca1 and Abcg1) and the rate-limiting enzyme in bile acid synthesis (Cyp7a1). d Plasma PCSK9 protein levels. e–f Immunoblot analysis and relative quantification of hepatic Pcsk9, Ldlr, Hmgcr and Gapdh protein levels (n = 5). Statistical testing was done with one-way ANOVA and Tukey test for multiple comparisons. $p < 0.05 between chow and at least two other groups. *p < 0.05, *p < 0.01 between indicated groups Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35788891), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Proprotein Convertase 9/PCSK9 by Western Blot

Detection of Proprotein Convertase 9/PCSK9 by Western Blot

Fishmeal feeding leads to circulating PCSK9. Expression of hepatic genes important for steroid hormone synthesis and cholesterol metabolism in mice fed fishmeal, salmon filet or beef (n = 10). a Validation RNA sequencing analysis with RT-qPCR analysis of selected genes identified as low expressed in fishmeal compared to salmon or beef (Hmgcr, Pcsk9, Fabp5, Fdsp, Chrna4). b Expression of mRNAs encoding genes regulating cholesterol synthesis (Srebf2) and lipogenesis (Srebf1c and Mlxipl/Chrebp). c Expression of cholesterol metabolite sensors (Nr1h3/LXR alpha and Nr1h2/LXR beta ), cholesterol efflux transporters (Abca1 and Abcg1) and the rate-limiting enzyme in bile acid synthesis (Cyp7a1). d Plasma PCSK9 protein levels. e–f Immunoblot analysis and relative quantification of hepatic Pcsk9, Ldlr, Hmgcr and Gapdh protein levels (n = 5). Statistical testing was done with one-way ANOVA and Tukey test for multiple comparisons. $p < 0.05 between chow and at least two other groups. *p < 0.05, *p < 0.01 between indicated groups Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35788891), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse liver tissue and rat embryonic cortical neurons

Reviewed Applications

Read 4 reviews rated 4.5 using AF3985 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Proprotein Convertase 9/PCSK9

PC9 (Proprotein convertase subtilisin type 9; also PCSK9 and NARC-1) is a 70‑74 kDa, secreted glycoprotein member of the proteinase K subfamily of subtilases. PC9 functions as a protease that cleaves proproteins at non-basic residues. It also binds to, and promotes, the degradation of LDLR, VLDLR and ApoER2. The mouse PC9 precursor is 694 amino acid (aa) in length, and contains a signal sequence, a 14 kDa, 121 aa prodomain (aa 35‑155), and a 56‑58 kDa, 539 aa mature region (aa 156‑694). The 58 kDa mature region arises from autocatalysis in the ER, and contains a subtilisin-like catalytic domain (aa 166‑432), a modified P-domain (aa 433‑529) and a C-terminal Cys-rich region (aa 530‑694). The prosegment and mature regions remain noncovalently associated. There is one additional PC9 isoform that shows an alternative start site 13 aa upstream of the standard site coupled to a deletion of aa 18‑20. Over aa 156‑694, mouse PC9 shares 93% and 79% aa identity with rat and human PC9, respectively.

Alternate Names

FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9

Entrez Gene IDs

255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)

Gene Symbol

PCSK9

UniProt

Q80W65

Additional Proprotein Convertase 9/PCSK9 Products

Product Documents for Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody

For research use only

Related Research Areas

Obesity
Serine Proteases and Regulators

Citations for Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody

Customer Reviews for Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody (4)

4.5 out of 5
4 Customer Ratings
5 Stars
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Name: Adrianne Pittman
    Verified Customer | Posted 04/26/2017
  • Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Purified protein
    Species: Mouse
    Verified Customer | Posted 02/22/2016
    Purified mouse Pcsk9 was used for immunoprecipitation (IP) validation. 5 ug AF3985 per IP incubation, works very well. This Ab may be further diluted for each IP.
    Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody AF3985
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Primary mouse hepatocytes
    Species: Mouse
    Verified Customer | Posted 12/19/2014
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver homogenates sample
    Species: Mouse
    Verified Customer | Posted 12/19/2014

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