Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody

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Detection of Mouse and Rat Proprotein Convertase 9/PCSK9 by Western Blot.
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Product Details
Citations (9)
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Reviews (2)

Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody Summary

Species Reactivity
Mouse, Rat
Detects mouse and rat Proprotein Convertase 9/PCSK9 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Proprotein Convertase 9
Accession # Q80W65
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat Proprotein Convertase 9/PCSK9 antibody by Western Blot. View Larger

Detection of Mouse and Rat Proprotein Convertase 9/PCSK9 by Western Blot. Western blot shows lysates of mouse liver tissue and rat embryonic cortical neuron. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Proprotein Convertase 9/PCSK9 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Proprotein Convertase 9/PCSK9

PC9 (Proprotein convertase subtilisin type 9; also PCSK9 and NARC-1) is a 70‑74 kDa, secreted glycoprotein member of the proteinase K subfamily of subtilases. PC9 functions as a protease that cleaves proproteins at non-basic residues. It also binds to, and promotes, the degradation of LDLR, VLDLR and ApoER2. The mouse PC9 precursor is 694 amino acid (aa) in length, and contains a signal sequence, a 14 kDa, 121 aa prodomain (aa 35‑155), and a 56‑58 kDa, 539 aa mature region (aa 156‑694). The 58 kDa mature region arises from autocatalysis in the ER, and contains a subtilisin-like catalytic domain (aa 166‑432), a modified P-domain (aa 433‑529) and a C-terminal Cys-rich region (aa 530‑694). The prosegment and mature regions remain noncovalently associated. There is one additional PC9 isoform that shows an alternative start site 13 aa upstream of the standard site coupled to a deletion of aa 18‑20. Over aa 156‑694, mouse PC9 shares 93% and 79% aa identity with rat and human PC9, respectively.

Entrez Gene IDs
255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)
Alternate Names
EC 3.4.21; EC; FH3; FH3neural apoptosis regulated convertase 1; FHCL3; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9

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Citations for Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Rosa26-LSL-dCas9-VPR: a versatile mouse model for tissue specific and simultaneous activation of multiple genes for drug discovery
    Authors: D Pakalnišky, T Schönberge, B Strobel, B Stierstorf, T Lamla, M Schuler, M Lenter
    Scientific Reports, 2022-11-10;12(1):19268.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
    Applications: Simple Western
  2. A multienzyme S-nitrosylation cascade regulates cholesterol homeostasis
    Authors: CT Stomberski, NM Venetos, HL Zhou, Z Qian, BR Collison, SJ Field, RT Premont, JS Stamler
    Cell Reports, 2022-10-25;41(4):111538.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: ICC, Western Blot
  3. Accumulation of cholesterol, triglycerides and ceramides in hepatocellular carcinomas of diethylnitrosamine injected mice
    Authors: EM Haberl, R Pohl, L Rein-Fisch, M Höring, S Krautbauer, G Liebisch, C Buechler
    Lipids in Health and Disease, 2021-10-10;20(1):135.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. In vivo adenine base editing of PCSK9 in macaques reduces LDL cholesterol levels
    Authors: T Rothgangl, MK Dennis, PJC Lin, R Oka, D Witzigmann, L Villiger, W Qi, M Hruzova, L Kissling, D Lenggenhag, C Borrelli, S Egli, N Frey, N Bakker, JA Walker, AP Kadina, DV Victorov, M Pacesa, S Kreutzer, Z Kontarakis, A Moor, M Jinek, D Weissman, M Stoffel, R van Boxtel, K Holden, N Pardi, B Thöny, J Häberle, YK Tam, SC Semple, G Schwank
    Nature Biotechnology, 2021-05-19;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer
    Authors: X Liu, X Bao, M Hu, H Chang, M Jiao, J Cheng, L Xie, Q Huang, F Li, CY Li
    Nature, 2020-11-11;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Liver Pyruvate Kinase Promotes NAFLD/NASH in both Mice and Humans in a Sex-Specific Manner
    Authors: KC Krishnan, RR Floyd, S Sabir, DW Jayasekera, PV Leon-Mimil, AE Jones, AA Cortez, V Shravah, M Péterfy, L Stiles, S Canizales-, AS Divakaruni, A Huertas-Va, AJ Lusis
    Cell Mol Gastroenterol Hepatol, 2020-09-14;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo
    Authors: R Ibraheim, CQ Song, A Mir, N Amrani, W Xue, EJ Sontheimer
    Genome Biol., 2018-09-19;19(1):137.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. ?-Secretase Inhibition Lowers Plasma Triglyceride-Rich Lipoproteins by Stabilizing the LDL Receptor
    Authors: K Kim, IJ Goldberg, MJ Graham, M Sundaram, E Bertaggia, SX Lee, L Qiang, RA Haeusler, D Metzger, P Chambon, Z Yao, HN Ginsberg, UB Pajvani
    Cell Metab., 2018-03-22;0(0):.
    Species: Mouse, Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Heparan sulfate proteoglycans present PCSK9 to the LDL receptor
    Authors: C Gustafsen, D Olsen, J Vilstrup, S Lund, A Reinhardt, N Wellner, T Larsen, CBF Andersen, K Weyer, JP Li, PH Seeberger, S Thirup, P Madsen, S Glerup
    Nat Commun, 2017-09-11;8(1):503.
    Species: Hamster, Human
    Sample Types: Cell Culture Supernates, Cell Lysates
    Applications: Western Blot


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Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody
By Adrianne Pittman on 04/26/2017

Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody
By Anonymous on 02/22/2016
Application: IP Sample Tested: Purified protein Species: Mouse

Purified mouse Pcsk9 was used for immunoprecipitation (IP) validation.
5 ug AF3985 per IP incubation, works very well.
This Ab may be further diluted for each IP.