Mouse/Rat Testisin/Prss21 Antibody

  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects recombinant mouse Testisin/Prss21 in direct ELISAs and Western blots. Detects mouse and rat Testisin/Prss21 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Prss8 and rmPrss27 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Testisin/Prss21
    Gln28-Asn298
    Accession # Q9JHJ7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat Testisin/Prss21 by Western Blot. Western blot shows lysates of mouse testis tissue and rat testis tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Testisin/Prss21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6820) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Testisin/Prss21 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Testisin/Prss21 in Mouse Testis. Testisin/Prss21 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse Testisin/Prss21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6820) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. This application has not been tested in rat samples.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Testisin/Prss21
Testisin is a serine protease encoded by the PRSS21 gene. Testisin is similar in structure to tryptases, and is also known as Tryptase‑4 (1). It is predicted to have substrate specificity similar to that of trypsin (2). Testisin is most highly expressed in testicular germ cells, but is also present in eosinophils (3). Testisin is known to be required for spermatogenesis, and deficiencies of the enzyme can result in infertility (4, 5). Testisin is anchored to the cell surface through a C-terminal glycosylphosphatidylinositol anchor (6). Recombinant mouse Testisin is expressed with a truncated C-terminus, resulting in its secretion. The recombinant protein was purified as the zymogen and can undergo autoactivation under the appropriate conditions. Over aa 28-298, mouse Testisin shares 92% aa identity with rat Testisin.
  • References:
    1. Wong, G.W. et al. (2001) J. Biol. Chem. 276:20648.
    2. Hooper, J.D. et al. (2000) Biochim. Biophys. Acta 1492:63.
    3. Inoue, M. et al. (1998) Biochem. Biophys.  Res. Comm. 252:307.
    4. Netzel-Arnett, S. et al. (2009) Biol. Reprod. 81:921.
    5. Kawano, N. et al. (2010) Biol. Reprod. 83:359.
    6. Honda, A. et al. (2002) J. Biol. Chem. 277:16976.
  • Entrez Gene IDs:
    10942 (Human); 57256 (Mouse)
  • Alternate Names:
    EC 3.4.21; Eosinophil serine protease 1; ESP1EC 3.4.21.-; ESP-1serine protease from eosinophils; protease, serine, 21 (testisin); Prss21; Serine protease 21; TEST1; TEST1testisin; Testisin
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