Mouse Serpin A3N Antibody

Catalog # Availability Size / Price Qty
AF4709
AF4709-SP

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Product Details
Citations (31)
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Reviews (1)

Mouse Serpin A3N Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Serpin A3N in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Serpin A1, recombinant human Serpin A3 and rmSerpin A5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin A3N
Phe21-Lys418
Accession # Q91WP6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Serpin A3N (Catalog # 4709-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Serpin A3N (Catalog # 4709-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A3N

Serpin A3N is a serine protease inhibitor that is structurally related to alpha 1-antichymotrypsin encoded by the SERPINA3 gene (1). Serpin A3N is highly expressed in brain, testis, lung, thymus, and spleen (2). Serpin A3N secreted by Sertoli cells may regulate the activity of locally produced Granzyme B (3). Granzyme B inhibition by Serpin A3N may therefore regulate Granzyme B-mediated killing by cytotoxic lymphocytes, providing a means to disable cell-mediated immune responses.

References
  1. Forsyth, S. et al. (2003) Genomics 81:336.
  2. Horvath, A. J. et al. (2004) J. Mol. Evol. 59:488.
  3. Hirst, C. E. et al. (2001) Mol. Hum. Reprod. 7:1133.
Entrez Gene IDs
20716 (Mouse); 24795 (Rat)
Alternate Names
A1A;A1AT;AAT;Alpha-1-antitrypsin;alpha1AT;nNIF;PI;PI1;PRO2275;SERPINA1; Serpin A3N; Spi2

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Citations for Mouse Serpin A3N Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelination
    Authors: J Hou, Y Zhou, Z Cai, M Terekhova, A Swain, PS Andhey, RM Guimaraes, A Ulezko Ant, T Qiu, S Sviben, G Strout, JAJ Fitzpatric, Y Chen, S Gilfillan, DH Kim, SJ Van Dyken, MN Artyomov, M Colonna
    Cell Reports, 2023-03-21;42(4):112293.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. SerpinA3N deficiency attenuates steatosis and enhances insulin signaling in male mice
    Authors: Melanie Tran, Md Golam Mostofa, Michael Picard, Jianguo Wu, Li Wang, Dong-Ju Shin
    Journal of Endocrinology
  3. Human early-onset dementia caused by DAP12 deficiency reveals a unique signature of dysregulated microglia
    Authors: Y Zhou, M Tada, Z Cai, PS Andhey, A Swain, KR Miller, S Gilfillan, MN Artyomov, M Takao, A Kakita, M Colonna
    Nature Immunology, 2023-01-19;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Microglia regulate central nervous system myelin growth and integrity
    Authors: NB McNamara, DAD Munro, N Bestard-Cu, A Uyeda, JFJ Bogie, A Hoffmann, RK Holloway, I Molina-Gon, KE Askew, S Mitchell, W Mungall, M Dodds, C Dittmayer, J Moss, J Rose, S Szymkowiak, L Amann, BW McColl, M Prinz, TL Spires-Jon, W Stenzel, K Horsburgh, JJA Hendriks, C Pridans, R Muramatsu, A Williams, J Priller, VE Miron
    Nature, 2022-12-14;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Medin co-aggregates with vascular amyloid-beta in Alzheimer's disease
    Authors: J Wagner, K Degenhardt, M Veit, N Louros, K Konstantou, A Skodras, K Wild, P Liu, U Obermüller, V Bansal, A Dalmia, LM Häsler, M Lambert, M De Vleesch, HA Davies, J Madine, D Kronenberg, R Feederle, D Del Turco, KPR Nilsson, T Lashley, T Deller, M Gearing, LC Walker, P Heutink, F Rousseau, J Schymkowit, M Jucker, JJ Neher
    Nature, 2022-11-16;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. CD8+ T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging
    Authors: T Kaya, N Mattugini, L Liu, H Ji, L Cantuti-Ca, J Wu, M Schifferer, J Groh, R Martini, S Besson-Gir, S Kaji, A Liesz, O Gokce, M Simons
    Nature Neuroscience, 2022-10-24;25(11):1446-1457.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Differential expression of tau species and the association with cognitive decline and synaptic loss in Alzheimer's disease
    Authors: Sivaprakasam R. Saroja, Abhijeet Sharma, Patrick R. Hof, Ana C. Pereira
    Alzheimer's & Dementia
  8. A shared disease-associated oligodendrocyte signature among multiple CNS pathologies
    Authors: M Kenigsbuch, P Bost, S Halevi, Y Chang, S Chen, Q Ma, R Hajbi, B Schwikowsk, B Bodenmille, H Fu, M Schwartz, I Amit
    Nature Neuroscience, 2022-06-27;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Microglia regulate central nervous system myelin growth and integrity
    Authors: NB McNamara, DAD Munro, N Bestard-Cu, A Uyeda, JFJ Bogie, A Hoffmann, RK Holloway, I Molina-Gon, KE Askew, S Mitchell, W Mungall, M Dodds, C Dittmayer, J Moss, J Rose, S Szymkowiak, L Amann, BW McColl, M Prinz, TL Spires-Jon, W Stenzel, K Horsburgh, JJA Hendriks, C Pridans, R Muramatsu, A Williams, J Priller, VE Miron
    Nature, 2022;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Integrated Multiomics Analysis Identifies a Novel Biomarker Associated with Prognosis in Intracerebral Hemorrhage
    Authors: Y Shen, W Yang, X Xiong, X Li, Z Xiao, J Yu, F Liu, S Gui, X Xie, F Lv, L Zhao, L Hu, A Manaenko, P Xie, Q Li
    Oxidative Medicine and Cellular Longevity, 2021-12-14;2021(0):2510847.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. The role of Serpina3n in the reversal effect of ATRA on dexamethasone-inhibited osteogenic differentiation in mesenchymal stem cells
    Authors: Hai-Tao Jiang, Rui Deng, Yan Deng, Mao Nie, Yi-Xuan Deng, Hong-Hong Luo et al.
    Stem Cell Research & Therapy
  12. Adult-onset CNS myelin sulfatide deficiency is sufficient to cause Alzheimer's disease-like neuroinflammation and cognitive impairment
    Authors: S Qiu, JP Palavicini, J Wang, NS Gonzalez, S He, E Dustin, C Zou, L Ding, A Bhattachar, CE Van Skike, V Galvan, JL Dupree, X Han
    Molecular Neurodegeneration, 2021-09-15;16(1):64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Waning efficacy in a long-term AAV-mediated gene therapy study in the murine model of Krabbe disease
    Authors: Gregory J. Heller, Michael S. Marshall, Yazan Issa, Jeffrey N. Marshall, Duc Nguyen, Emily Rue et al.
    Molecular Therapy
  14. Longitudinal single cell transcriptomics reveals a role for Serpina3n-mediated resolution of inflammation in a mouse colitis model
    Authors: YT Ho, T Shimbo, E Wijaya, T Kitayama, S Takaki, K Ikegami, K Miyashita, Y Ouchi, E Takaki, R Yamamoto, Y Kaneda, K Tamai
    Cellular and Molecular Gastroenterology and Hepatology, 2021-04-13;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Immunoprecipitation, Western Blot
  15. Polymorphic SERPINA3 prolongs oligomeric state of amyloid beta
    Authors: MM Akbor, N Kurosawa, H Nakayama, A Nakatani, K Tomobe, Y Chiba, M Ueno, M Tanaka, Y Nomura, M Isobe
    PLoS ONE, 2021-03-04;16(3):e0248027.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. CSF SERPINA3 Levels Are Elevated in Patients With Progressive MS
    Authors: Nicolás Fissolo, Clara Matute-Blanch, Mohamoud Osman, Carme Costa, Rucsanda Pinteac, Berta Miró et al.
    Neurology - Neuroimmunology Neuroinflammation
  17. Oxysterols present in Alzheimer's disease brain induce synaptotoxicity by activating astrocytes: A major role for lipocalin-2
    Authors: E Staurenghi, V Cerrato, P Gamba, G Testa, S Giannelli, V Leoni, C Caccia, A Buffo, W Noble, BG Perez-Niev, G Leonarduzz
    Redox Biology, 2020-12-17;39(0):101837.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  18. Marked Increased Production of Acute Phase Reactants by Skeletal Muscle during Cancer Cachexia
    Authors: IS Massart, G Paulissen, A Loumaye, P Lause, SA Pötgens, MM Thibaut, E Balan, L Deldicque, A Atfi, E Louis, D Gruson, LB Bindels, MA Meuwis, JP Thissen
    Cancers (Basel), 2020-10-31;12(11):.
    Species: Mouse
    Sample Types: Cell Lystates
    Applications: Western Blot
  19. Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi
    Authors: SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh
    Nat Commun, 2020-08-14;11(1):4102.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Muscle NAD+ depletion and Serpina3n as molecular determinants of murine cancer cachexia - the effects of blocking myostatin and activins
    Authors: JJ Hulmi, F Penna, N Pöllänen, TA Nissinen, J Hentilä, L Euro, JH Lautaoja, R Ballarò, R Soliymani, M Baumann, O Ritvos, E Pirinen, M Lalowski
    Mol Metab, 2020-06-26;0(0):101046.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. Alzheimer’s Risk Factors Age, APOE Genotype, and Sex Drive Distinct Molecular Pathways
    Authors: Na Zhao, Yingxue Ren, Yu Yamazaki, Wenhui Qiao, Fuyao Li, Lindsey M. Felton et al.
    Neuron
  22. Disease-associated astrocytes in Alzheimer's disease and aging
    Authors: N Habib, C McCabe, S Medina, M Varshavsky, D Kitsberg, R Dvir-Szter, G Green, D Dionne, L Nguyen, JL Marshall, F Chen, F Zhang, T Kaplan, A Regev, M Schwartz
    Nat. Neurosci., 2020-04-27;23(6):701-706.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Retinal endothelial cell phenotypic modifications during experimental autoimmune uveitis: a transcriptomic approach
    Authors: DA Lipski, V Foucart, R Dewispelae, LE Caspers, M Defrance, C Bruyns, F Willermain
    BMC Ophthalmol, 2020-03-17;20(1):106.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  24. Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease
    Authors: Y Zhou, WM Song, PS Andhey, A Swain, T Levy, KR Miller, PL Poliani, M Cominelli, S Grover, S Gilfillan, M Cella, TK Ulland, K Zaitsev, A Miyashita, T Ikeuchi, M Sainouchi, A Kakita, DA Bennett, JA Schneider, MR Nichols, SA Beausoleil, JD Ulrich, DM Holtzman, MN Artyomov, M Colonna
    Nat. Med., 2020-01-13;26(1):131-142.
    Species: Mouse
    Sample Types: Tissue
    Applications: IHC
  25. SerpinA3N is a novel hypothalamic gene upregulated by a high-fat diet and leptin in mice
    Authors: Domenico Sergi, Fiona M. Campbell, Christine Grant, Amanda C. Morris, Eva-Maria Bachmair, Christiane Koch et al.
    Genes & Nutrition
  26. Diet induced obesity is independent of metabolic endotoxemia and TLR4 signalling, but markedly increases hypothalamic expression of the acute phase protein, SerpinA3N
    Authors: MJ Dalby, G Aviello, AW Ross, AW Walker, P Barrett, PJ Morgan
    Sci Rep, 2018-10-23;8(1):15648.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Increased Serpina3n release into circulation during glucocorticoid-mediated muscle atrophy
    Authors: Marine Gueugneau, Donatienne d'Hose, Caroline Barbé, Marie de Barsy, Pascale Lause, Dominique Maiter et al.
    Journal of Cachexia, Sarcopenia and Muscle
  28. Differential overexpression of SERPINA3 in human prion diseases
    Authors: S Vanni, F Moda, M Zattoni, E Bistaffa, E De Cecco, M Rossi, G Giaccone, F Tagliavini, S Haïk, JP Deslys, G Zanusso, JW Ironside, I Ferrer, GG Kovacs, G Legname
    Sci Rep, 2017-11-15;7(1):15637.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  29. The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase.
    Authors: Vicuna L, Strochlic D, Latremoliere A, Bali K, Simonetti M, Husainie D, Prokosch S, Riva P, Griffin R, Njoo C, Gehrig S, Mall M, Arnold B, Devor M, Woolf C, Liberles S, Costigan M, Kuner R
    Nat Med, 2015-04-27;21(5):518-23.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  30. Serpina3n accelerates tissue repair in a diabetic mouse model of delayed wound healing
    Authors: I Hsu, L G Parkinson, Y Shen, A Toro, T Brown, H Zhao et al.
    Cell Death & Disease
  31. The development of diet-induced obesity and glucose intolerance in C57BL/6 mice on a high-fat diet consists of distinct phases.
    Authors: Williams, Lynda M, Campbell, Fiona M, Drew, Janice E, Koch, Christia, Hoggard, Nigel, Rees, William, Kamolrat, Torkamol, Thi Ngo, Ha, Steffensen, Inger-Li, Gray, Stuart R, Tups, Alexande
    PLoS ONE, 2014-08-29;9(8):e106159.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot

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Mouse Serpin A3N Antibody
By Anonymous on 07/08/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult brain Species: Mouse

The red showed serpin A3N in the ARC of hypothalamus. Scale bar: 50 um.

Primary dilution: 1 : 250
Secondary donkey anti goat 555, dilution 1 : 1000