Mouse Serpin A3N Antibody

Name: Mouse Serpin A3N Antibody
Brand: R&D Systems
SKU: AF4709-SP
Rating: 5 (1 reviews)

AF4709 8 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4709
AF4709-SP

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Product Details

Citations (8)

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Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Serpin A3N Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin A3N in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Serpin A1, recombinant human Serpin A3 and rmSerpin A5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin A3N
Phe21-Lys418
Accession # Q91WP6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Serpin A3N (Catalog # 4709-PI)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Serpin A3N (Catalog # 4709-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A3N

Serpin A3N is a serine protease inhibitor that is structurally related to alpha 1-antichymotrypsin encoded by the SERPINA3 gene (1). Serpin A3N is highly expressed in brain, testis, lung, thymus, and spleen (2). Serpin A3N secreted by Sertoli cells may regulate the activity of locally produced Granzyme B (3). Granzyme B inhibition by Serpin A3N may therefore regulate Granzyme B-mediated killing by cytotoxic lymphocytes, providing a means to disable cell-mediated immune responses.

References

Forsyth, S. et al. (2003) Genomics 81:336.
Horvath, A. J. et al. (2004) J. Mol. Evol. 59:488.
Hirst, C. E. et al. (2001) Mol. Hum. Reprod. 7:1133.

Entrez Gene IDs

20716 (Mouse); 24795 (Rat)

Alternate Names

Serpin A3N; Spi2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Serpin A3N Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease
Authors: Y Zhou, WM Song, PS Andhey, A Swain, T Levy, KR Miller, PL Poliani, M Cominelli, S Grover, S Gilfillan, M Cella, TK Ulland, K Zaitsev, A Miyashita, T Ikeuchi, M Sainouchi, A Kakita, DA Bennett, JA Schneider, MR Nichols, SA Beausoleil, JD Ulrich, DM Holtzman, MN Artyomov, M Colonna
Nat. Med., 2020;26(1):131-142.
Species: Mouse
Sample Types: Tissue
Applications: IHC
Retinal endothelial cell phenotypic modifications during experimental autoimmune uveitis: a transcriptomic approach
Authors: DA Lipski, V Foucart, R Dewispelae, LE Caspers, M Defrance, C Bruyns, F Willermain
BMC Ophthalmol, 2020;20(1):106.
Species: Mouse
Sample Types: Whole Tissue
Applications: IF
Marked Increased Production of Acute Phase Reactants by Skeletal Muscle during Cancer Cachexia
Authors: IS Massart, G Paulissen, A Loumaye, P Lause, SA Pötgens, MM Thibaut, E Balan, L Deldicque, A Atfi, E Louis, D Gruson, LB Bindels, MA Meuwis, JP Thissen
Cancers (Basel), 2020;12(11):.
Species: Mouse
Sample Types: Cell Lystates
Applications: Western Blot
Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi
Authors: SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh
Nat Commun, 2020;11(1):4102.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Diet induced obesity is independent of metabolic endotoxemia and TLR4 signalling, but markedly increases hypothalamic expression of the acute phase protein, SerpinA3N
Authors: MJ Dalby, G Aviello, AW Ross, AW Walker, P Barrett, PJ Morgan
Sci Rep, 2018;8(1):15648.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Differential overexpression of SERPINA3 in human prion diseases
Authors: S Vanni, F Moda, M Zattoni, E Bistaffa, E De Cecco, M Rossi, G Giaccone, F Tagliavini, S Haïk, JP Deslys, G Zanusso, JW Ironside, I Ferrer, GG Kovacs, G Legname
Sci Rep, 2017;7(1):15637.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase.
Authors: Vicuna L, Strochlic D, Latremoliere A, Bali K, Simonetti M, Husainie D, Prokosch S, Riva P, Griffin R, Njoo C, Gehrig S, Mall M, Arnold B, Devor M, Woolf C, Liberles S, Costigan M, Kuner R
Nat Med, 2015;21(5):518-23.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
The development of diet-induced obesity and glucose intolerance in C57BL/6 mice on a high-fat diet consists of distinct phases.
Authors: Williams, Lynda M, Campbell, Fiona M, Drew, Janice E, Koch, Christia, Hoggard, Nigel, Rees, William, Kamolrat, Torkamol, Thi Ngo, Ha, Steffensen, Inger-Li, Gray, Stuart R, Tups, Alexande
PLoS ONE, 2014;9(8):e106159.
Species: Mouse
Sample Types: Plasma
Applications: Western Blot