Mouse Serpin A3N Antibody

Catalog # Availability Size / Price Qty
AF4709
AF4709-SP
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Mouse Serpin A3N Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Serpin A3N in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Serpin A1, recombinant human Serpin A3 and rmSerpin A5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin A3N
Phe21-Lys418
Accession # Q91WP6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Serpin A3N (Catalog # 4709-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Serpin A3N (Catalog # 4709-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A3N

Serpin A3N is a serine protease inhibitor that is structurally related to alpha 1-antichymotrypsin encoded by the SERPINA3 gene (1). Serpin A3N is highly expressed in brain, testis, lung, thymus, and spleen (2). Serpin A3N secreted by Sertoli cells may regulate the activity of locally produced Granzyme B (3). Granzyme B inhibition by Serpin A3N may therefore regulate Granzyme B-mediated killing by cytotoxic lymphocytes, providing a means to disable cell-mediated immune responses.

References
  1. Forsyth, S. et al. (2003) Genomics 81:336.
  2. Horvath, A. J. et al. (2004) J. Mol. Evol. 59:488.
  3. Hirst, C. E. et al. (2001) Mol. Hum. Reprod. 7:1133.
Entrez Gene IDs
20716 (Mouse); 24795 (Rat)
Alternate Names
Serpin A3N; Spi2

Product Datasheets

Citations for Mouse Serpin A3N Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease
    Authors: Y Zhou, WM Song, PS Andhey, A Swain, T Levy, KR Miller, PL Poliani, M Cominelli, S Grover, S Gilfillan, M Cella, TK Ulland, K Zaitsev, A Miyashita, T Ikeuchi, M Sainouchi, A Kakita, DA Bennett, JA Schneider, MR Nichols, SA Beausoleil, JD Ulrich, DM Holtzman, MN Artyomov, M Colonna
    Nat. Med., 2020;26(1):131-142.
    Species: Mouse
    Sample Types: Tissue
    Applications: IHC
  2. Retinal endothelial cell phenotypic modifications during experimental autoimmune uveitis: a transcriptomic approach
    Authors: DA Lipski, V Foucart, R Dewispelae, LE Caspers, M Defrance, C Bruyns, F Willermain
    BMC Ophthalmol, 2020;20(1):106.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  3. Marked Increased Production of Acute Phase Reactants by Skeletal Muscle during Cancer Cachexia
    Authors: IS Massart, G Paulissen, A Loumaye, P Lause, SA Pötgens, MM Thibaut, E Balan, L Deldicque, A Atfi, E Louis, D Gruson, LB Bindels, MA Meuwis, JP Thissen
    Cancers (Basel), 2020;12(11):.
    Species: Mouse
    Sample Types: Cell Lystates
    Applications: Western Blot
  4. Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi
    Authors: SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh
    Nat Commun, 2020;11(1):4102.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Diet induced obesity is independent of metabolic endotoxemia and TLR4 signalling, but markedly increases hypothalamic expression of the acute phase protein, SerpinA3N
    Authors: MJ Dalby, G Aviello, AW Ross, AW Walker, P Barrett, PJ Morgan
    Sci Rep, 2018;8(1):15648.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Differential overexpression of SERPINA3 in human prion diseases
    Authors: S Vanni, F Moda, M Zattoni, E Bistaffa, E De Cecco, M Rossi, G Giaccone, F Tagliavini, S Haïk, JP Deslys, G Zanusso, JW Ironside, I Ferrer, GG Kovacs, G Legname
    Sci Rep, 2017;7(1):15637.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase.
    Authors: Vicuna L, Strochlic D, Latremoliere A, Bali K, Simonetti M, Husainie D, Prokosch S, Riva P, Griffin R, Njoo C, Gehrig S, Mall M, Arnold B, Devor M, Woolf C, Liberles S, Costigan M, Kuner R
    Nat Med, 2015;21(5):518-23.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. The development of diet-induced obesity and glucose intolerance in C57BL/6 mice on a high-fat diet consists of distinct phases.
    Authors: Williams, Lynda M, Campbell, Fiona M, Drew, Janice E, Koch, Christia, Hoggard, Nigel, Rees, William, Kamolrat, Torkamol, Thi Ngo, Ha, Steffensen, Inger-Li, Gray, Stuart R, Tups, Alexande
    PLoS ONE, 2014;9(8):e106159.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot

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Reviews for Mouse Serpin A3N Antibody

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Mouse Serpin A3N Antibody
By Anonymous on 07/08/2021
Application: ICC/IF Sample Tested: Adult brain Species: Mouse

The red showed serpin A3N in the ARC of hypothalamus. Scale bar: 50 um.

Primary dilution: 1 : 250
Secondary donkey anti goat 555, dilution 1 : 1000