Mouse Serpin A3N Antibody
Summary
Preparation and Storage
Reconstitution Calculator
Background
Product Datasheets
Related Research Areas
Mouse Serpin A3N Antibody Summary
Species Reactivity
Mouse
Specificity
Detects mouse Serpin A3N in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Serpin A1, recombinant human Serpin A3 and rmSerpin A5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin A3N
Phe21-Lys418
Accession # Q91WP6
Phe21-Lys418
Accession # Q91WP6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
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Applications
Recommended Concentration
Sample
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Serpin A3N (Catalog # 4709-PI), see our available Western blot detection antibodies
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A3N
Serpin A3N is a serine protease inhibitor that is structurally related to alpha 1-antichymotrypsin encoded by the SERPINA3 gene (1). Serpin A3N is highly expressed in brain, testis, lung, thymus, and spleen (2). Serpin A3N secreted by Sertoli cells may regulate the activity of locally produced Granzyme B (3). Granzyme B inhibition by Serpin A3N may therefore regulate Granzyme B-mediated killing by cytotoxic lymphocytes, providing a means to disable cell-mediated immune responses.
Product Datasheets
Citations for Mouse Serpin A3N Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
17
Citations: Showing 1 - 10
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A shared disease-associated oligodendrocyte signature among multiple CNS pathologies
Authors: M Kenigsbuch, P Bost, S Halevi, Y Chang, S Chen, Q Ma, R Hajbi, B Schwikowsk, B Bodenmille, H Fu, M Schwartz, I Amit
Nature Neuroscience, 2022;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Integrated Multiomics Analysis Identifies a Novel Biomarker Associated with Prognosis in Intracerebral Hemorrhage
Authors: Y Shen, W Yang, X Xiong, X Li, Z Xiao, J Yu, F Liu, S Gui, X Xie, F Lv, L Zhao, L Hu, A Manaenko, P Xie, Q Li
Oxidative Medicine and Cellular Longevity, 2021;2021(0):2510847.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Polymorphic SERPINA3 prolongs oligomeric state of amyloid beta
Authors: MM Akbor, N Kurosawa, H Nakayama, A Nakatani, K Tomobe, Y Chiba, M Ueno, M Tanaka, Y Nomura, M Isobe
PLoS ONE, 2021;16(3):e0248027.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Adult-onset CNS myelin sulfatide deficiency is sufficient to cause Alzheimer's disease-like neuroinflammation and cognitive impairment
Authors: S Qiu, JP Palavicini, J Wang, NS Gonzalez, S He, E Dustin, C Zou, L Ding, A Bhattachar, CE Van Skike, V Galvan, JL Dupree, X Han
Molecular Neurodegeneration, 2021;16(1):64.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Longitudinal single cell transcriptomics reveals a role for Serpina3n-mediated resolution of inflammation in a mouse colitis model
Authors: YT Ho, T Shimbo, E Wijaya, T Kitayama, S Takaki, K Ikegami, K Miyashita, Y Ouchi, E Takaki, R Yamamoto, Y Kaneda, K Tamai
Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation -
Retinal endothelial cell phenotypic modifications during experimental autoimmune uveitis: a transcriptomic approach
Authors: DA Lipski, V Foucart, R Dewispelae, LE Caspers, M Defrance, C Bruyns, F Willermain
BMC Ophthalmol, 2020;20(1):106.
Species: Mouse
Sample Types: Whole Tissue
Applications: IF -
Marked Increased Production of Acute Phase Reactants by Skeletal Muscle during Cancer Cachexia
Authors: IS Massart, G Paulissen, A Loumaye, P Lause, SA Pötgens, MM Thibaut, E Balan, L Deldicque, A Atfi, E Louis, D Gruson, LB Bindels, MA Meuwis, JP Thissen
Cancers (Basel), 2020;12(11):.
Species: Mouse
Sample Types: Cell Lystates
Applications: Western Blot -
Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi
Authors: SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh
Nat Commun, 2020;11(1):4102.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease
Authors: Y Zhou, WM Song, PS Andhey, A Swain, T Levy, KR Miller, PL Poliani, M Cominelli, S Grover, S Gilfillan, M Cella, TK Ulland, K Zaitsev, A Miyashita, T Ikeuchi, M Sainouchi, A Kakita, DA Bennett, JA Schneider, MR Nichols, SA Beausoleil, JD Ulrich, DM Holtzman, MN Artyomov, M Colonna
Nat. Med., 2020;26(1):131-142.
Species: Mouse
Sample Types: Tissue
Applications: IHC -
Muscle NAD+ depletion and Serpina3n as molecular determinants of murine cancer cachexia - the effects of blocking myostatin and activins
Authors: JJ Hulmi, F Penna, N Pöllänen, TA Nissinen, J Hentilä, L Euro, JH Lautaoja, R Ballarò, R Soliymani, M Baumann, O Ritvos, E Pirinen, M Lalowski
Mol Metab, 2020;0(0):101046.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Disease-associated astrocytes in Alzheimer's disease and aging
Authors: N Habib, C McCabe, S Medina, M Varshavsky, D Kitsberg, R Dvir-Szter, G Green, D Dionne, L Nguyen, JL Marshall, F Chen, F Zhang, T Kaplan, A Regev, M Schwartz
Nat. Neurosci., 2020;23(6):701-706.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Oxysterols present in Alzheimer's disease brain induce synaptotoxicity by activating astrocytes: A major role for lipocalin-2
Authors: E Staurenghi, V Cerrato, P Gamba, G Testa, S Giannelli, V Leoni, C Caccia, A Buffo, W Noble, BG Perez-Niev, G Leonarduzz
Redox Biology, 2020;39(0):101837.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi
Authors: SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh
Nat Commun, 2020;11(1):4102.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Diet induced obesity is independent of metabolic endotoxemia and TLR4 signalling, but markedly increases hypothalamic expression of the acute phase protein, SerpinA3N
Authors: MJ Dalby, G Aviello, AW Ross, AW Walker, P Barrett, PJ Morgan
Sci Rep, 2018;8(1):15648.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Differential overexpression of SERPINA3 in human prion diseases
Authors: S Vanni, F Moda, M Zattoni, E Bistaffa, E De Cecco, M Rossi, G Giaccone, F Tagliavini, S Haïk, JP Deslys, G Zanusso, JW Ironside, I Ferrer, GG Kovacs, G Legname
Sci Rep, 2017;7(1):15637.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase.
Authors: Vicuna L, Strochlic D, Latremoliere A, Bali K, Simonetti M, Husainie D, Prokosch S, Riva P, Griffin R, Njoo C, Gehrig S, Mall M, Arnold B, Devor M, Woolf C, Liberles S, Costigan M, Kuner R
Nat Med, 2015;21(5):518-23.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
The development of diet-induced obesity and glucose intolerance in C57BL/6 mice on a high-fat diet consists of distinct phases.
Authors: Williams, Lynda M, Campbell, Fiona M, Drew, Janice E, Koch, Christia, Hoggard, Nigel, Rees, William, Kamolrat, Torkamol, Thi Ngo, Ha, Steffensen, Inger-Li, Gray, Stuart R, Tups, Alexande
PLoS ONE, 2014;9(8):e106159.
Species: Mouse
Sample Types: Plasma
Applications: Western Blot
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Mouse Serpin A3N Antibody
By Anonymous
on 07/08/2021
Application: ICC/IF
Sample Tested: Adult brain
Species:
Mouse
The red showed serpin A3N in the ARC of hypothalamus. Scale bar: 50 um.
Primary dilution: 1 : 250
Secondary donkey anti goat 555, dilution 1 : 1000
