Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Serpin A3N Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin A3N
Phe21-Lys418
Accession # Q91WP6

Specificity

Detects mouse Serpin A3N in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Serpin A1, recombinant human Serpin A3 and rmSerpin A5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Serpin A3N Antibody

Serpin A3N in Mouse Brain Tissue.

Serpin A3N in Mouse Brain Tissue.

Serpin A3N + labelled oligodendrocytes can be seen in the Corpus callosum of cuprizone-intoxicated mice. Primary antibody: Serpin A3N 1:1000, 18h incubation at 4°CSecondary antibody: rabbit anti goat, 1:200, 21°C, 1h incubation. Visualization via DAB. Image from a verified customer review.
Detection of Mouse Serpin A3N by Western Blot

Detection of Mouse Serpin A3N by Western Blot

HS diet increased the serpina3n expression in the hippocampus of APP/PS1 mice. (A) GSEA showed that the HS diet intervention changed the status of axon guidance, neuroactive ligand–receptor interaction, oxidoreductase activity, and blood vessel diameter. (B) A radar map of differential gene expression levels. Serpina3n is most significantly changed, and its mRNA expression is the highest among the serpin family. (C) Protein level of serpina3n was assessed using Western blotting; GAPDH was used as a control. n = 4 biologically independent animals. (D) mRNA levels of the serpin family in HS- and ND-treated APP/PS1 mice were compared; serpina3n is the most remarkably changed mRNA. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/21/11731), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Serpin A3N Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin A3N (Catalog # 4709-PI), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Serpin A3N (Catalog # 4709-PI)

Reviewed Applications

Read 2 reviews rated 5 using AF4709 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin A3N

Serpin A3N is a serine protease inhibitor that is structurally related to alpha 1-antichymotrypsin encoded by the SERPINA3 gene (1). Serpin A3N is highly expressed in brain, testis, lung, thymus, and spleen (2). Serpin A3N secreted by Sertoli cells may regulate the activity of locally produced Granzyme B (3). Granzyme B inhibition by Serpin A3N may therefore regulate Granzyme B-mediated killing by cytotoxic lymphocytes, providing a means to disable cell-mediated immune responses.

References

  1. Forsyth, S. et al. (2003) Genomics 81:336.
  2. Horvath, A. J. et al. (2004) J. Mol. Evol. 59:488.
  3. Hirst, C. E. et al. (2001) Mol. Hum. Reprod. 7:1133.

Alternate Names

Spi2

Entrez Gene IDs

20716 (Mouse); 24795 (Rat)

Gene Symbol

SERPINA3N

UniProt

Q91WP6 (Mouse)

Additional Serpin A3N Products

Product Documents for Mouse Serpin A3N Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Serpin A3N Antibody

For research use only

Citations for Mouse Serpin A3N Antibody

Customer Reviews for Mouse Serpin A3N Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
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1 Stars
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Customer Images

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  • Mouse Serpin A3N Antibody on mouse brain
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 06/17/2025
    Serpin A3N + labelled oligodendrocytes can be seen in the Corpus callosum of cuprizone-intoxicated mice
    Primary antibody: Serpina3n 1:1000, 18h incubation at 4°C Secondary antibody: rabbit anti goat, 1:200, 21°C, 1h incubation Visualisation via DAB
    Mouse Serpin A3N Antibody AF4709
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Mouse Serpin A3N Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult brain
    Species: Mouse
    Verified Customer | Posted 07/08/2021
    The red showed serpin A3N in the ARC of hypothalamus. Scale bar: 50 um. Primary dilution: 1 : 250 Secondary donkey anti goat 555, dilution 1 : 1000
    Mouse Serpin A3N Antibody AF4709

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