Mouse serum amyloid A protein-4 (SAA4; also SAA5) is a 14 kDa member of the SAA family of proteins. It is a constitutively produced apolipoprotein component of HDL that may mediate HDL-VLDL interactions. Mature mouse SAA4 is 112 amino acids (aa) in length. There are no notable motifs. Although human SAA4 is glycosylated, mouse SAA4 is not. Human SAA4 also shows truncation of its Lys-Lys-Tyr C-terminus. The mouse C-terminus is distantly related with a Glu-Lys-Phe tripeptide, but it is unknown if it is proteolytically processed. Over aa 19‑130, mouse SAA4 shares 57% and 85% aa identity with mature human and rat SAA4, respectively.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse Serum Amyloid A4
Asp19-Phe130
Accession # P31532
Asp19-Phe130
Accession # P31532
Specificity
Detects mouse Serum Amyloid A4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant human Serum Amyloid A4 is observed and less than 1% cross-reactivity with recombinant mouse Serum Amyloid A1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Serum Amyloid A4 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serum Amyloid A4, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serum Amyloid A4, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Serum Amyloid A4
Sample: Recombinant Mouse Serum Amyloid A4
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Serum Amyloid A4
Alternate Names
C-SAA, SAA4, SAA5
Gene Symbol
SAA4
UniProt
Additional Serum Amyloid A4 Products
Product Documents for Mouse Serum Amyloid A4 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Serum Amyloid A4 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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