Mouse SOST/Sclerostin Antibody

Catalog # Availability Size / Price Qty
AF1589
AF1589-SP
Detection  of Human and Mouse SOST/Sclerostin by Western Blot.
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Product Details
Citations (29)
FAQs
Supplemental Products
Reviews (1)

Mouse SOST/Sclerostin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SOST/Sclerostin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SOST is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SOST/Sclerostin
Gln24-Tyr211
Accession # NP_077769
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human and Mouse SOST/Sclerostin by Western Blot. View Larger

Detection of Human and Mouse SOST/Sclerostin by Western Blot. Western blot shows lysates of human bone marrow and mouse bone marrow. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF1589) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOST/Sclerostin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry SOST/Sclerostin in Mouse Embryo. View Larger

SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (adrenal gland) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry SOST/Sclerostin in Mouse Embryo. View Larger

SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOST/Sclerostin

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Human and mouse SOST share 88% amino acid identity (1-3).

References
  1. Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
  2. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
  3. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.
Entrez Gene IDs
50964 (Human); 74499 (Mouse)
Alternate Names
sclerostin; SOST; VBCHsclerosteosis

Product Datasheets

Citations for Mouse SOST/Sclerostin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Sclerostin is upregulated in the early stage of chondrogenic differentiation, but not required in endochondral ossification in vitro
    Authors: Y Yamaguchi, K Kumagai, S Imai, K Miyatake, T Saito
    PLoS ONE, 2018;13(8):e0201839.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  2. Bone adaptation in response to treadmill exercise in young and adult mice
    Authors: JD Gardinier, N Rostami, L Juliano, C Zhang
    Bone Rep, 2018;8(0):29-37.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Transient peak-strain matching partially recovers the age-impaired mechanoadaptive cortical bone response
    Authors: B Javaheri, A Carriero, M Wood, R De Souza, PD Lee, S Shefelbine, AA Pitsillide
    Sci Rep, 2018;8(1):6636.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Gs? Controls Cortical Bone Quality by Regulating Osteoclast Differentiation via cAMP/PKA and ?-Catenin Pathways
    Authors: G Ramaswamy, H Kim, D Zhang, V Lounev, JY Wu, Y Choi, FS Kaplan, RJ Pignolo, EM Shore
    Sci Rep, 2017;7(0):45140.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement
    Authors: R Shu, D Bai, T Sheu, Y He, X Yang, C Xue, Y He, M Zhao, X Han
    PLoS ONE, 2017;12(1):e0167312.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Zoledronate promotes bone formation by blocking osteocyte-osteoblast communication during bone defect healing
    Authors: P Cui, H Liu, J Sun, N Amizuka, Q Sun, M Li
    Histol. Histopathol., 2017;0(0):11893.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  7. Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone
    Authors: A Sakurai, T Hasegawa, A Kudo, Z Shen, T Nagai, M Abe, T Yoshida, H Hongo, T Yamamoto, T Yamamoto, K Oda, PHL Freitas, M Li, H Sano, N Amizuka
    Biomed. Res., 2017;38(4):257-267.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC paraffin embedded
  8. Short-term intermittent administration of parathyroid hormone facilitates osteogenesis by different mechanisms in cancellous and cortical bone
    Authors: K Ogura, T Iimura, Y Makino, A Sugie-Oya, A Takakura, R Takao-Kawa, T Ishizuya, K Moriyama, A Yamaguchi
    Bone Rep, 2016;5(0):7-14.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. Suppression of Sclerostin Alleviates Radiation-Induced Bone Loss by Protecting Bone Forming Cells and Their Progenitors through Distinct Mechanisms
    J Bone Miner Res, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  10. Distorted Patterns of Dentinogenesis and Eruption in Msx2 Null Mutants: Involvement of Sost/Sclerostin
    Am J Pathol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  11. Inflammatory bowel disease in a rodent model alters osteocyte protein levels controlling bone turnover
    Authors: S A Bloomfield
    J. Bone Miner. Res., 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Osteocytes mediate the anabolic actions of canonical Wnt/beta-catenin signaling in bone.
    Authors: Tu X, Delgado-Calle J, Condon K, Maycas M, Zhang H, Carlesso N, Taketo M, Burr D, Plotkin L, Bellido T
    Proc Natl Acad Sci U S A, 2015;112(5):E478-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  13. Parathyroid Hormone Induces Bone Cell Motility and Loss of Mature Osteocyte Phenotype through L-Calcium Channel Dependent and Independent Mechanisms.
    Authors: Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L
    PLoS ONE, 2015;10(5):e0125731.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  14. Androgens inhibit the osteogenic response to mechanical loading in adult male mice.
    Authors: Sinnesael M, Laurent M, Jardi F, Dubois V, Deboel L, Delisser P, Behets G, D'Haese P, Carmeliet G, Claessens F, Vanderschueren D
    Endocrinology, 2015;156(4):1343-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  15. Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/beta-CATENIN SIGNALING PATHWAY.
    Authors: Beier E, Sheu T, Dang D, Holz J, Ubayawardena R, Babij P, Puzas J
    J Biol Chem, 2015;290(29):18216-26.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  16. The Wnt Inhibitor Sclerostin Is Up-regulated by Mechanical Unloading in Osteocytes in Vitro.
    Authors: Spatz J, Wein M, Gooi J, Qu Y, Garr J, Liu S, Barry K, Uda Y, Lai F, Dedic C, Balcells-Camps M, Kronenberg H, Babij P, Pajevic P
    J Biol Chem, 2015;290(27):16744-58.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  17. The osteoblast to osteocyte transition: epigenetic changes and response to the vitamin D3 hormone.
    Authors: St John, Hillary, Bishop, Kathleen, Meyer, Mark B, Benkusky, Nancy A, Leng, Ning, Kendziorski, Christin, Bonewald, Lynda F, Pike, J Wesley
    Mol Endocrinol, 2014;28(7):1150-65.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  18. Disrupted bone remodeling leads to cochlear overgrowth and hearing loss in a mouse model of fibrous dysplasia.
    Authors: Akil, Omar, Hall-Glenn, Faith, Chang, Jolie, Li, Alfred, Chang, Wenhan, Lustig, Lawrence, Alliston, Tamara, Hsiao, Edward C
    PLoS ONE, 2014;9(5):e94989.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  19. Inhibition of GSK-3beta rescues the impairments in bone formation and mechanical properties associated with fracture healing in osteoblast selective connexin 43 deficient mice.
    Authors: Loiselle A, Lloyd S, Paul E, Lewis G, Donahue H
    PLoS ONE, 2013;8(11):e81399.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  20. miR-218 directs a Wnt signaling circuit to promote differentiation of osteoblasts and osteomimicry of metastatic cancer cells.
    Authors: Hassan, Mohammad, Maeda, Yukiko, Taipaleenmaki, Hanna, Zhang, Weibing, Jafferji, Mohammad, Gordon, Jonathan, Li, Zhaoyong, Croce, Carlo M, van Wijnen, Andre J, Stein, Janet L, Stein, Gary S, Lian, Jane B
    J Biol Chem, 2012;287(50):42084-92.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  21. Matrix metalloproteinase-13 is required for osteocytic perilacunar remodeling and maintains bone fracture resistance.
    J. Bone Miner. Res., 2012;27(9):1936-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  22. Repression of osteocyte Wnt/β-catenin signaling is an early event in the progression of renal osteodystrophy.
    Authors: Sabbagh Y, Graciolli FG, O'Brien S, Tang W, Dos Reis LM, Ryan S, Phillips L, Boulanger J, Song W, Bracken C, Liu S, Ledbetter S, Dechow P, Canziani ME, Carvalho AB, Jorgetti V, Moyses RM, Schiavi SC
    J. Bone Miner. Res., 2012;27(8):1757-72.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  23. The Appearance and Modulation of Osteocyte Marker Expression during Calcification of Vascular Smooth Muscle Cells.
    Authors: Zhu D, Mackenzie NC, Millan JL, Farquharson C, MacRae VE
    PLoS ONE, 2011;6(5):e19595.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  24. Conditional deletion of Bmpr1a in differentiated osteoclasts increases osteoblastic bone formation, increasing volume of remodeling bone in mice.
    Authors: Okamoto M, Murai J, Imai Y, Ikegami D, Kamiya N, Kato S, Mishina Y, Yoshikawa H, Tsumaki N
    J. Bone Miner. Res., 2011;26(10):2511-22.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  25. Parathyroid hormone receptor signaling in osteocytes increases the expression of fibroblast growth factor-23 in vitro and in vivo.
    Authors: Rhee Y, Bivi N, Farrow E, Lezcano V, Plotkin LI, White KE, Bellido T
    Bone, 2011;49(4):636-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  26. Localization of SOST/sclerostin in cementocytes in vivo and in mineralizing periodontal ligament cells in vitro.
    Authors: Jager A, Gotz W, Lossdorfer S, Rath-Deschner B
    J. Periodont. Res., 2010;45(2):246-54.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  27. Distinct modes of inhibition by sclerostin on bone morphogenetic protein and Wnt signaling pathways.
    Authors: Krause C, Korchynskyi O, de Rooij K, Weidauer SE, de Gorter DJ, van Bezooijen RL, Hatsell S, Economides AN, Mueller TD, Lowik CW, ten Dijke P
    J. Biol. Chem., 2010;285(53):41614-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  28. Parathyroid hormone signaling through low-density lipoprotein-related protein 6.
    Authors: Wan M, Yang C, Li J, Wu X, Yuan H, Ma H, He X, Nie S, Chang C, Cao X
    Genes Dev., 2008;22(21):2968-79.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Fresh
  29. Control of the SOST bone enhancer by PTH using MEF2 transcription factors.
    Authors: Leupin O, Kramer I, Collette NM, Loots GG, Natt F, Kneissel M, Keller H
    J. Bone Miner. Res., 2007;22(12):1957-67.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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Mouse SOST/Sclerostin Antibody
By Anonymous on 04/21/2017
Application: WB Sample Tested: Bone Extracts,Bone marrow,Bone marrow cells,Liver tissue Species: Mouse