Mouse SOST/Sclerostin Antibody

(25 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse SOST/Sclerostin in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human SOST is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse SOST/Sclerostin
    Gln24-Tyr211
    Accession # NP_077769
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse SOST/Sclerostin by Western Blot. Western blot shows lysates of human bone marrow and mouse bone marrow. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF1589) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOST/Sclerostin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (kidney) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to glomeruli. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (adrenal gland) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOST/Sclerostin

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Human and mouse SOST share 88% amino acid identity (1-3).

  • References:
    1. Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
    2. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
    3. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.
  • Entrez Gene IDs:
    50964 (Human); 74499 (Mouse)
  • Alternate Names:
    sclerostin; SOST; VBCHsclerosteosis
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone
    Authors: A Sakurai, T Hasegawa, A Kudo, Z Shen, T Nagai, M Abe, T Yoshida, H Hongo, T Yamamoto, T Yamamoto, K Oda, PHL Freitas, M Li, H Sano, N Amizuka
    Biomed. Res., 2017;38(4):257-267.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. Zoledronate promotes bone formation by blocking osteocyte-osteoblast communication during bone defect healing
    Authors: P Cui, H Liu, J Sun, N Amizuka, Q Sun, M Li
    Histol. Histopathol., 2017;0(0):11893.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Gs? Controls Cortical Bone Quality by Regulating Osteoclast Differentiation via cAMP/PKA and ?-Catenin Pathways
    Authors: G Ramaswamy, H Kim, D Zhang, V Lounev, JY Wu, Y Choi, FS Kaplan, RJ Pignolo, EM Shore
    Sci Rep, 2017;7(0):45140.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement
    Authors: R Shu, D Bai, T Sheu, Y He, X Yang, C Xue, Y He, M Zhao, X Han
    PLoS ONE, 2017;12(1):e0167312.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Short-term intermittent administration of parathyroid hormone facilitates osteogenesis by different mechanisms in cancellous and cortical bone
    Authors: K Ogura, T Iimura, Y Makino, A Sugie-Oya, A Takakura, R Takao-Kawa, T Ishizuya, K Moriyama, A Yamaguchi
    Bone Rep, 2016;5(0):7-14.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  6. Suppression of Sclerostin Alleviates Radiation-Induced Bone Loss by Protecting Bone Forming Cells and Their Progenitors through Distinct Mechanisms
    J Bone Miner Res, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Distorted Patterns of Dentinogenesis and Eruption in Msx2 Null Mutants: Involvement of Sost/Sclerostin
    Am J Pathol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  8. The Wnt Inhibitor Sclerostin Is Up-regulated by Mechanical Unloading in Osteocytes in Vitro.
    Authors: Spatz J, Wein M, Gooi J, Qu Y, Garr J, Liu S, Barry K, Uda Y, Lai F, Dedic C, Balcells-Camps M, Kronenberg H, Babij P, Pajevic P
    J Biol Chem, 2015;290(27):16744-58.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  9. Androgens inhibit the osteogenic response to mechanical loading in adult male mice.
    Authors: Sinnesael M, Laurent M, Jardi F, Dubois V, Deboel L, Delisser P, Behets G, D'Haese P, Carmeliet G, Claessens F, Vanderschueren D
    Endocrinology, 2015;156(4):1343-53.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  10. Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/beta-CATENIN SIGNALING PATHWAY.
    Authors: Beier E, Sheu T, Dang D, Holz J, Ubayawardena R, Babij P, Puzas J
    J Biol Chem, 2015;290(29):18216-26.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. Parathyroid Hormone Induces Bone Cell Motility and Loss of Mature Osteocyte Phenotype through L-Calcium Channel Dependent and Independent Mechanisms.
    Authors: Prideaux M, Dallas S, Zhao N, Johnsrud E, Veno P, Guo D, Mishina Y, Harris S, Bonewald L
    PLoS ONE, 2015;10(5):e0125731.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  12. Osteocytes mediate the anabolic actions of canonical Wnt/beta-catenin signaling in bone.
    Authors: Tu X, Delgado-Calle J, Condon K, Maycas M, Zhang H, Carlesso N, Taketo M, Burr D, Plotkin L, Bellido T
    Proc Natl Acad Sci U S A, 2015;112(5):E478-86.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  13. Disrupted bone remodeling leads to cochlear overgrowth and hearing loss in a mouse model of fibrous dysplasia.
    Authors: Akil, Omar, Hall-Glenn, Faith, Chang, Jolie, Li, Alfred, Chang, Wenhan, Lustig, Lawrence, Alliston, Tamara, Hsiao, Edward C
    PLoS ONE, 2014;9(5):e94989.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  14. The osteoblast to osteocyte transition: epigenetic changes and response to the vitamin D3 hormone.
    Authors: St John, Hillary, Bishop, Kathleen, Meyer, Mark B, Benkusky, Nancy A, Leng, Ning, Kendziorski, Christin, Bonewald, Lynda F, Pike, J Wesley
    Mol Endocrinol, 2014;28(7):1150-65.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  15. Inhibition of GSK-3beta rescues the impairments in bone formation and mechanical properties associated with fracture healing in osteoblast selective connexin 43 deficient mice.
    Authors: Loiselle A, Lloyd S, Paul E, Lewis G, Donahue H
    PLoS ONE, 2013;8(11):e81399.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. miR-218 directs a Wnt signaling circuit to promote differentiation of osteoblasts and osteomimicry of metastatic cancer cells.
    Authors: Hassan, Mohammad, Maeda, Yukiko, Taipaleenmaki, Hanna, Zhang, Weibing, Jafferji, Mohammad, Gordon, Jonathan, Li, Zhaoyong, Croce, Carlo M, van Wijnen, Andre J, Stein, Janet L, Stein, Gary S, Lian, Jane B
    J Biol Chem, 2012;287(50):42084-92.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  17. Matrix metalloproteinase-13 is required for osteocytic perilacunar remodeling and maintains bone fracture resistance.
    J. Bone Miner. Res., 2012;27(9):1936-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  18. Repression of osteocyte Wnt/β-catenin signaling is an early event in the progression of renal osteodystrophy.
    Authors: Sabbagh Y, Graciolli FG, O'Brien S, Tang W, Dos Reis LM, Ryan S, Phillips L, Boulanger J, Song W, Bracken C, Liu S, Ledbetter S, Dechow P, Canziani ME, Carvalho AB, Jorgetti V, Moyses RM, Schiavi SC
    J. Bone Miner. Res., 2012;27(8):1757-72.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  19. The Appearance and Modulation of Osteocyte Marker Expression during Calcification of Vascular Smooth Muscle Cells.
    Authors: Zhu D, Mackenzie NC, Millan JL, Farquharson C, MacRae VE
    PLoS ONE, 2011;6(5):e19595.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  20. Conditional deletion of Bmpr1a in differentiated osteoclasts increases osteoblastic bone formation, increasing volume of remodeling bone in mice.
    Authors: Okamoto M, Murai J, Imai Y, Ikegami D, Kamiya N, Kato S, Mishina Y, Yoshikawa H, Tsumaki N
    J. Bone Miner. Res., 2011;26(10):2511-22.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  21. Parathyroid hormone receptor signaling in osteocytes increases the expression of fibroblast growth factor-23 in vitro and in vivo.
    Authors: Rhee Y, Bivi N, Farrow E, Lezcano V, Plotkin LI, White KE, Bellido T
    Bone, 2011;49(4):636-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  22. Localization of SOST/sclerostin in cementocytes in vivo and in mineralizing periodontal ligament cells in vitro.
    Authors: Jager A, Gotz W, Lossdorfer S, Rath-Deschner B
    J. Periodont. Res., 2010;45(2):246-54.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  23. Distinct modes of inhibition by sclerostin on bone morphogenetic protein and Wnt signaling pathways.
    Authors: Krause C, Korchynskyi O, de Rooij K, Weidauer SE, de Gorter DJ, van Bezooijen RL, Hatsell S, Economides AN, Mueller TD, Lowik CW, ten Dijke P
    J. Biol. Chem., 2010;285(53):41614-26.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  24. Parathyroid hormone signaling through low-density lipoprotein-related protein 6.
    Authors: Wan M, Yang C, Li J, Wu X, Yuan H, Ma H, He X, Nie S, Chang C, Cao X
    Genes Dev., 2008;22(21):2968-79.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  25. Control of the SOST bone enhancer by PTH using MEF2 transcription factors.
    Authors: Leupin O, Kramer I, Collette NM, Loots GG, Natt F, Kneissel M, Keller H
    J. Bone Miner. Res., 2007;22(12):1957-67.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 25 Citations
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 1 review)

Have you used Mouse SOST/Sclerostin Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Mouse SOST/Sclerostin Antibody.

We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Western Blot.

Filter by Ratings
Excellent
(1)
All Ratings
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
 WB Mouse Anonymous 04/21/2017
Show

Summary

ApplicationWestern Blot
Sample TestedBone Extracts,Bone marrow,Bone marrow cells,Liver tissue
SpeciesMouse

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information