Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SOST/Sclerostin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SOST/Sclerostin
Gln24-Tyr211
Accession # NP_077769

Specificity

Detects mouse SOST/Sclerostin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SOST is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse SOST/Sclerostin Antibody

Detection of Human and Mouse SOST/Sclerostin antibody by Western Blot.

Detection of Human and Mouse SOST/Sclerostin by Western Blot.

Western blot shows lysates of human bone marrow and mouse bone marrow. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOST/Sclerostin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SOST/Sclerostin antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

SOST/Sclerostin in Mouse Embryo.

SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (adrenal gland) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
SOST/Sclerostin antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

SOST/Sclerostin in Mouse Embryo.

SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Mouse SOST/Sclerostin Antibody by Immunohistochemistry

Detection of Mouse Mouse SOST/Sclerostin Antibody by Immunohistochemistry

Mice were treated with vehicle or 50 μg/kg/day for 14 days and the effect on bone architecture examined using μCT (a). Further sections of bone were stained with H&E (b) or immunostained for cathepsin K (c) or sclerostin (d). Large images are 10 times and insert images are 40 times magnification. Scale bars in large images equals 100 μm and in insert images 50 μm. Osteoclasts were defined as large, strongly cathepsin K stained cells on a bone surface and are indicated by arrows in figure c. Examples of sclerostin positive osteocytes are indicated by arrows in figure d. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28249797), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse SOST/Sclerostin Antibody by Immunohistochemistry

Detection of Mouse Mouse SOST/Sclerostin Antibody by Immunohistochemistry

Mice were treated with vehicle or 50 μg/kg/day for 28 days. The fibula was sectioned and immunostained for sclerostin. Scale bars equals 100 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28249797), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SOST/Sclerostin by Immunohistochemistry

Detection of Mouse SOST/Sclerostin by Immunohistochemistry

Loading-induced increased PGE2 secretion with a decrease in SOST expression was inhibited in cKO mice. a, b ELISA analysis of PGE2 level in bone marrow-flushed tibial diaphysis (a) and serum (b) after 5-day mechanical loading. n = 6 per group. c Representative COX-2 immunohistostaining (black arrows) and d, e quantification of COX-2-positive osteocytes in diaphyseal 37% cortical bone. Scale bar, 30 μm. n = 6 per group. f Representative SOST immunohistostaining (black arrows) and g, h quantification of SOST-positive osteocytes in diaphyseal 37% cortical bone. Scale bar, 30 μm. n = 6 per group. i Representative beta -catenin immunohistostaining (black arrows) and j, k quantification of beta -catenin-positive osteoblasts on endosteal surface of diaphyseal 37% cortical bone. Scale bar, 30 μm. n = 5–6 per group. l–n Relative gene expression of Sost (l), COX-2 (m), and beta -catenin (n) was determined by RT-qPCR in the tibial diaphysis of WT and cKO mice. n = 5 per group. Mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Paired t test was done for loaded and contralateral tibias and unpaired t test was done for loaded or control tibias between WT and cKO mice Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35851577), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SOST/Sclerostin by Western Blot

Detection of SOST/Sclerostin by Western Blot

OCY‐EVs Transported to the Brain under Physiological & Pathological Conditions.Representative E) WB images of DMP1 & SOST expression. Scale bar: 100 µm. beta ‐actin was used as loading control. OB, Osteoblast; BMM, bone marrow macrophage. n = 3 per group. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35508803), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SOST/Sclerostin by Western Blot

Detection of SOST/Sclerostin by Western Blot

OCY‐EVs Transported to the Brain under Physiological & Pathological Conditions.Representative H) WB images of osteocyte markers (SOST & DMP1) & EVs classical markers (TSG101 & Flotillin‐1). Scale bar: 100 nm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35508803), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SOST/Sclerostin by Immunohistochemistry

Detection of SOST/Sclerostin by Immunohistochemistry

Localized sclerostin accumulation and increased expression of PLR-related proteins in 20-week-old male db/db diabetic mice cortical bone. Immunohistochemical staining shows increased expression of CTSK (A), MMP-13 (B), and sclerostin (C) within the osteocyte LCS of db/db mice compared to WT mice. Linear regression analysis (D) reveals a significant positive correlation between sclerostin expression and CTSK and MMP-13 using combined data from both groups. Data are presented as mean ± SD. Statistical analysis was performed using an unpaired Student’s t-test for (A–C) (n = 5 mice per group) and Pearson correlation for (D). *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40352664), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse SOST/Sclerostin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (adrenal gland) and immersion fixed frozen sections of mouse embryo (kidney)

Western Blot

2 µg/mL
Sample: Human bone marrow and mouse bone marrow

Reviewed Applications

Read 7 reviews rated 4.6 using AF1589 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOST/Sclerostin

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Human and mouse SOST share 88% amino acid identity (1-3).

References

  1. Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
  2. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
  3. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.

Alternate Names

sclerostin, VBCHsclerosteosis

Entrez Gene IDs

50964 (Human); 74499 (Mouse)

Gene Symbol

SOST

UniProt

NP_077769

Additional SOST/Sclerostin Products

Product Documents for Mouse SOST/Sclerostin Antibody

Certificate of Analysis

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Product Specific Notices for Mouse SOST/Sclerostin Antibody

For research use only

Citations for Mouse SOST/Sclerostin Antibody

Customer Reviews for Mouse SOST/Sclerostin Antibody (7)

4.6 out of 5
7 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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Showing  1 - 5 of 7 reviews Showing All
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  • Mouse SOST/Sclerostin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Mouse
    Verified Customer | Posted 08/31/2023
    Mouse SOST/Sclerostin Antibody AF1589
  • Mouse SOST/Sclerostin Antibody
    Name: liaa Li
    Application: Immunohistochemistry
    Sample Tested: bone
    Species: Mouse
    Verified Customer | Posted 07/07/2021
    Mouse SOST/Sclerostin Antibody AF1589
  • Mouse SOST/Sclerostin Antibody
    Name: Xue Yuan
    Application: Immunohistochemistry
    Sample Tested: BONE
    Species: Mouse
    Verified Customer | Posted 06/27/2021
    Mouse SOST/Sclerostin Antibody AF1589
  • Mouse SOST/Sclerostin Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Recombinant protein
    Species: Mouse
    Verified Customer | Posted 06/25/2021
    Mouse SOST/Sclerostin Antibody AF1589
  • Mouse SOST/Sclerostin Antibody
    Name: Beata Lecka-Czernik
    Application: Western Blot
    Sample Tested: Bone Extracts, bone marrow, Bone marrow cells and Liver tissue
    Species: Mouse
    Verified Customer | Posted 04/21/2017
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23494985
    Species: Mouse
    Verified Customer | Posted 01/05/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 20336693
    Species: Other
    Verified Customer | Posted 01/05/2015

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