Mouse SPARC-like 1/SPARCL1 Antibody

Catalog # Availability Size / Price Qty
AF2836
AF2836-SP
Detection of Mouse SPARC-like 1/SPARCL1 by Immunocytochemistry/Immunofluorescence
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Product Details
Citations (12)
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Mouse SPARC-like 1/SPARCL1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SPARC-like 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human SPARC-like 1 is observed and less than 1% cross-reactivity with recombinant mouse SPARC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SPARC-like 1/SPARCL1 (R&D Systems, Catalog # 4547-SL)
Ile17-Phe650
Accession # P70663
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse SPARC-like 1/SPARCL1 (Catalog # 4547-SL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry/ Immunofluorescence Detection of Mouse SPARC-like 1/SPARCL1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse SPARC-like 1/SPARCL1 by Immunocytochemistry/Immunofluorescence Hevin expression by astrocytes is developmentally regulated in the cortex.(A) Representative Western blots showing the developmental timeline for hevin expression in mouse cortex and hippocampus (tubulin was used as a loading control). (B) Quantification of Western blot analysis of hevin expression shows high expression between P15–P25. Data is presented as fold change compared to P1 levels (n = 3 animals per age; p < 0.05; one-way ANOVA with Dunnett's post hoc test). (C) Schematic diagram of a coronal slice through mouse brain shows the synaptic zone of primary visual cortex (V1) where EM, IHC and Golgi-cox staining analyses were performed. Layer II/III neurons of the visual cortex heavily project their dendrites to this region (D) IHC staining reveals that hevin expression (green) overlaps with all astrocytes (left, arrow) and a small subset of neurons (middle, asterisk) in V1, with no overlap seen with microglia (right, arrowhead). Cell-specific markers in red: Aldh1L1-EGFP for astrocytes, NeuN for neurons, Iba1 for microglia. Scale bar, 50 µm. (E) The rarely occurring GFAP+ astrocytes (red) in healthy visual cortex also express hevin (green). Scale bar, 10 µm.DOI:http://dx.doi.org/10.7554/eLife.04047.003 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25517933), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse SPARC-like 1/SPARCL1 by Western Blot View Larger

Detection of Mouse SPARC-like 1/SPARCL1 by Western Blot Hevin expression by astrocytes is developmentally regulated in the cortex.(A) Representative Western blots showing the developmental timeline for hevin expression in mouse cortex and hippocampus (tubulin was used as a loading control). (B) Quantification of Western blot analysis of hevin expression shows high expression between P15–P25. Data is presented as fold change compared to P1 levels (n = 3 animals per age; p < 0.05; one-way ANOVA with Dunnett's post hoc test). (C) Schematic diagram of a coronal slice through mouse brain shows the synaptic zone of primary visual cortex (V1) where EM, IHC and Golgi-cox staining analyses were performed. Layer II/III neurons of the visual cortex heavily project their dendrites to this region (D) IHC staining reveals that hevin expression (green) overlaps with all astrocytes (left, arrow) and a small subset of neurons (middle, asterisk) in V1, with no overlap seen with microglia (right, arrowhead). Cell-specific markers in red: Aldh1L1-EGFP for astrocytes, NeuN for neurons, Iba1 for microglia. Scale bar, 50 µm. (E) The rarely occurring GFAP+ astrocytes (red) in healthy visual cortex also express hevin (green). Scale bar, 10 µm.DOI:http://dx.doi.org/10.7554/eLife.04047.003 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25517933), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse SPARC-like 1/SPARCL1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse SPARC-like 1/SPARCL1 by Immunocytochemistry/Immunofluorescence Hevin expression by astrocytes is developmentally regulated in the cortex.(A) Representative Western blots showing the developmental timeline for hevin expression in mouse cortex and hippocampus (tubulin was used as a loading control). (B) Quantification of Western blot analysis of hevin expression shows high expression between P15–P25. Data is presented as fold change compared to P1 levels (n = 3 animals per age; p < 0.05; one-way ANOVA with Dunnett's post hoc test). (C) Schematic diagram of a coronal slice through mouse brain shows the synaptic zone of primary visual cortex (V1) where EM, IHC and Golgi-cox staining analyses were performed. Layer II/III neurons of the visual cortex heavily project their dendrites to this region (D) IHC staining reveals that hevin expression (green) overlaps with all astrocytes (left, arrow) and a small subset of neurons (middle, asterisk) in V1, with no overlap seen with microglia (right, arrowhead). Cell-specific markers in red: Aldh1L1-EGFP for astrocytes, NeuN for neurons, Iba1 for microglia. Scale bar, 50 µm. (E) The rarely occurring GFAP+ astrocytes (red) in healthy visual cortex also express hevin (green). Scale bar, 10 µm.DOI:http://dx.doi.org/10.7554/eLife.04047.003 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25517933), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPARC-like 1/SPARCL1

SPARCL1 (Secreted Protein, Acidic and Rich in Cysteines-like 1), also known as hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins (1, 2). SPARCL1 is an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver (3, 4). Mouse SPARCL1 contains a 16 amino acid (aa) signal sequence and a 634 aa mature region that contains four domains: a 403 aa N-terminal acidic region, a 23 aa follistatin-like domain, a 55 aa kazal-like segment and a 148 aa calcium binding domain that contains two EF hand motifs (3, 4). Mouse mature SPARCL1 shares 89%, 67%, 63%, 61%, 60%, and 58% aa identity with rat, human, equine, canine, porcine, and bovine SPARCL1, respectively. The follistatin-like, kazal-like and calcium-binding domains of SPARCL1 show 61% aa identity with corresponding regions of SPARC. SPARCL1 is predicted at 75 kDa, but migrates at ~130 kDa, which has been explained either by disulfide-linked homodimerization or by glycosylation and high acidity (3 - 5). Some truncated forms have been reported. In mouse, a 55 kDa C‑terminal fragment is the only form in kidney and represent a portion of SPARCL1 in other tissues (6). In humans, a 25 kDa form is increased in liver tumors that are encapsulated, while the full-length form is downregulated in many epithelial cell-derived tumors (7, 8). SPARCL1 inhibits adhesion and spreading on a variety of substrates (5, 9). It is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma (10). In tonsillar high endothelial venules (HEV), SPARCL1 may induce endothelial cell dissociation, promoting extravasation (3). SPARCL1 binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin (6, 11).

References
  1. Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
  2. Sullivan, M.M. and E.H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991.
  3. Girard, J.P. and T.A. Springer (1995) Immunity 2:113.
  4. Bendik, I. et al. (1998) Cancer Res. 58:626.
  5. Brekken, R.A. et al. (2004) J. Histochem. Cytochem. 52:735.
  6. Hambrock, H.O. et al. (2003) J. Biol. Chem. 278:11351.
  7. Lau, C.P. et al. (2006) J. Pathol. 210:469.
  8. Isler, S.G. et al. (2001) Int. J. Oncol. 18:521.
  9. Girard, J.P. and T.A. Springer (1996) J. Biol. Chem. 271:4511.
  10. Gongidi, V. et al. (2004) Neuron 41:57.
  11. Sullivan, M.M. et al. (2006) J. Biol. Chem. 281:27621.
Entrez Gene IDs
8404 (Human); 13602 (Mouse)
Alternate Names
Hevin; High endothelial venule protein; MAST 9; MAST9; PIG33; proliferation-inducing protein 33; SC1; SPARC like 1; SPARCL1; SPARC-like 1 (hevin); SPARC-like 1 (mast9, hevin); SPARC-like 1; SPARC-like protein 1

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Citations for Mouse SPARC-like 1/SPARCL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Comprehensive Profiling of Early Neoplastic Gastric Microenvironment Modifications and Biodynamics in Impaired BMP-Signaling FoxL1+-Telocytes
    Authors: AB Alfonso, V Pomerleau, VR Nicolás, J Raisch, CM Jurkovic, FM Boisvert, N Perreault
    Biomedicines, 2022-12-22;11(1):.
    Species: Mouse
    Sample Types: Tissue Homogenate
    Applications: Western Blot
  2. SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice
    Authors: Y Wang, S Zhang, Z Lan, V Doan, B Kim, S Liu, M Zhu, VL Hull, S Rihani, CL Zhang, JA Gray, F Guo
    Cell Reports, 2022-12-20;41(12):111842.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Cocaine preference and neuroadaptations are maintained by astrocytic NMDA receptors in the nucleus accumbens
    Authors: GP Shelkar, PJ Gandhi, J Liu, SM Dravid
    Science Advances, 2022-07-22;8(29):eabo6574.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Autism-associated mutation in Hevin/Sparcl1 induces endoplasmic reticulum stress through structural instability
    Authors: T Taketomi, T Yasuda, R Morita, J Kim, Y Shigeta, C Eroglu, R Harada, F Tsuruta
    Scientific Reports, 2022-07-13;12(1):11891.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  5. Gene silencing by EZH2 suppresses TGF-beta activity within the decidua to avert pregnancy-adverse wound healing at the maternal-fetal interface
    Authors: I Osokine, J Siewiera, D Rideaux, S Ma, T Tsukui, A Erlebacher
    Cell Reports, 2022-02-01;38(5):110329.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Lineage-tracing and translatomic analysis of damage-inducible mitotic cochlear progenitors identifies candidate genes regulating regeneration
    Authors: T Udagawa, PJ Atkinson, B Milon, JM Abitbol, Y Song, M Sperber, E Huarcaya N, M Scheibinge, R Elkon, R Hertzano, AG Cheng
    PloS Biology, 2021-11-10;19(11):e3001445.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics
    Authors: TA Jan, Y Eltawil, AH Ling, L Chen, DC Ellwanger, S Heller, AG Cheng
    Cell Reports, 2021-07-13;36(2):109358.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC, RNAScope
  8. Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea
    Authors: M Kubota, M Scheibinge, TA Jan, S Heller
    Cell Reports, 2021-01-19;34(3):108646.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility
    Authors: L Yu, Y Zheng, BJ Liu, MH Kang, JC Millar, DJ Rhee
    PLoS ONE, 2020-11-04;15(11):e0241294.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  10. Species-, organ- and cell-type-dependent expression of SPARCL1 in human and mouse tissues
    Authors: A Klingler, D Regensburg, C Tenkerian, N Britzen-La, A Hartmann, M Stürzl, E Naschberge
    PLoS ONE, 2020-05-21;15(5):e0233422.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Astrocytes refine cortical connectivity at dendritic spines.
    Authors: Risher, W Christ, Patel, Sagar, Kim, Il Hwan, Uezu, Akiyoshi, Bhagat, Srishti, Wilton, Daniel K, Pilaz, Louis-Ja, Singh Alvarado, Jonnatha, Calhan, Osman Y, Silver, Debra L, Stevens, Beth, Calakos, Nicole, Soderling, Scott H, Eroglu, Cagla
    Elife, 2014-12-17;3(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Processing of the matricellular protein hevin in mouse brain is dependent on ADAMTS4.
    Authors: Weaver MS, Workman G, Cardo-Vila M, Arap W, Pasqualini R, Sage EH
    J. Biol. Chem., 2009-12-15;285(8):5868-77.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Mouse SPARC-like 1/SPARCL1 Antibody
By Anonymous on 12/10/2023
Application: IHC Sample Tested: Lung tissue Species: Mouse