Mouse TSG-6 Antibody

Catalog # Availability Size / Price Qty
AF2326
AF2326-SP
TSG‑6  in Mouse Splenocytes.
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Citations (1)
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Mouse TSG-6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TSG-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human TSG-6 is observed and less than 2% cross-reactivity with recombinant mouse TSG-14 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TSG-6
Trp18-Leu275
Accession # O08859
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TSG‑6 (Catalog # 2326-TS)
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry TSG‑6  in Mouse Splenocytes. View Larger

TSG‑6 in Mouse Splenocytes. TSG‑6 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse TSG‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2326) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSG-6

TSG-6 (TNF-stimulated gene 6; also named TNFIP6) is a secreted, 35‑39 kDa group A member of the LINK-Module superfamily of proteins (1‑4). Mouse TSG-6 is synthesized as a 275 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 258 aa mature region (5, 6). The mature region has an N-terminal link module (aa 36‑129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (aa 135‑246). Link modules bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature mouse TSG-6 shares 97%, 94% and 94% aa identity with rat, human and canine TSG-6, respectively. Cells reported to express TGF-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA‑rich ECM. It does so by serving as an intermediary, or as a link between the individual subunits of the extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80‑85 kDa protein subunit from I alpha I (inter-alpha -inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a catalyst for the removal and transient binding of either HC chain. Each chain is subsequently transferred and covalently-linked to the surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10, 11, 12). This disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

References
  1. Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
  2. Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
  3. Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
  4. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  5. Fulop, C. et al. (1997) Gene 202:95.
  6. Fulop, C. et al. (2003) Development 130:2253.
  7. Kohda, D. et al. (1996) Cell 86:767.
  8. Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
  9. Salustri, A. et al. (2003) Development 131:1577.
  10. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  11. Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
  12. Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.
Long Name
Tumor Necrosis Factor-stimulated Gene Sequence 6
Entrez Gene IDs
7130 (Human); 21930 (Mouse)
Alternate Names
Hyaluronate-binding protein; TNF alpha-induced protein 6; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; TSG-6; TSG-6tumor necrosis factor alpha-inducible protein 6; TSG6tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-stimulated gene-6 protein

Product Datasheets

Citation for Mouse TSG-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mesenchymal Stromal Cells Inhibit Neutrophil Effector Functions in a Murine Model of Ocular Inflammation
    Authors: SK Mittal, A Mashaghi, A Amouzegar, M Li, W Foulsham, SK Sahu, SK Chauhan
    Invest. Ophthalmol. Vis. Sci., 2018;59(3):1191-1198.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut

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