Mouse TSG-6 Antibody

AF2326 1 Citation Datasheet / COA / SDS

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AF2326
AF2326-SP

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Mouse TSG-6 Antibody in Immunocytochemistry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TSG-6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TSG-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human TSG-6 is observed and less than 2% cross-reactivity with recombinant mouse TSG-14 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TSG-6
Trp18-Leu275
Accession # O08859

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TSG‑6 (Catalog # 2326-TS)

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry

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TSG‑6 in Mouse Splenocytes. TSG‑6 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse TSG‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2326) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSG-6

TSG-6 (TNF-stimulated gene 6; also named TNFIP6) is a secreted, 35‑39 kDa group A member of the LINK-Module superfamily of proteins (1‑4). Mouse TSG-6 is synthesized as a 275 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 258 aa mature region (5, 6). The mature region has an N-terminal link module (aa 36‑129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (aa 135‑246). Link modules bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature mouse TSG-6 shares 97%, 94% and 94% aa identity with rat, human and canine TSG-6, respectively. Cells reported to express TGF-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA‑rich ECM. It does so by serving as an intermediary, or as a link between the individual subunits of the extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80‑85 kDa protein subunit from I alpha I (inter-alpha -inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a catalyst for the removal and transient binding of either HC chain. Each chain is subsequently transferred and covalently-linked to the surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10, 11, 12). This disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

References

Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
Fulop, C. et al. (1997) Gene 202:95.
Fulop, C. et al. (2003) Development 130:2253.
Kohda, D. et al. (1996) Cell 86:767.
Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
Salustri, A. et al. (2003) Development 131:1577.
Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.

Long Name

Tumor Necrosis Factor-stimulated Gene Sequence 6

Entrez Gene IDs

7130 (Human); 21930 (Mouse)

Alternate Names

Hyaluronate-binding protein; TNF alpha-induced protein 6; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; TSG-6; TSG-6tumor necrosis factor alpha-inducible protein 6; TSG6tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-stimulated gene-6 protein

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Product Datasheet

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Citation for Mouse TSG-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Mesenchymal Stromal Cells Inhibit Neutrophil Effector Functions in a Murine Model of Ocular Inflammation
Authors: SK Mittal, A Mashaghi, A Amouzegar, M Li, W Foulsham, SK Sahu, SK Chauhan
Invest. Ophthalmol. Vis. Sci., 2018;59(3):1191-1198.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization