Mouse TSG-6 Antibody

• Species Reactivity
  Mouse
• Specificity
  Detects mouse TSG-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human TSG-6 is observed and less than 2% cross-reactivity with recombinant mouse TSG-14 is observed.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant mouse TSG-6
  Trp18-Leu275
  Accession # O08859
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.1 µg/mL
  Recombinant Mouse TSG‑6 (Catalog # 2326-TS)
• Immunocytochemistry
  5-15 µg/mL
  See below

Immunocytochemistry click image to view larger

TSG‑6 in Mouse Splenocytes. TSG‑6 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse TSG‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2326) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
  2. Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
  3. Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
  4. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  5. Fulop, C. et al. (1997) Gene 202:95.
  6. Fulop, C. et al. (2003) Development 130:2253.
  7. Kohda, D. et al. (1996) Cell 86:767.
  8. Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
  9. Salustri, A. et al. (2003) Development 131:1577.
  10. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  11. Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
  12. Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.

• Long Name:
  Tumor Necrosis Factor-stimulated Gene Sequence 6

• Entrez Gene IDs:
  7130 (Human); 21930 (Mouse)

• Alternate Names:
  Hyaluronate-binding protein; TNF alpha-induced protein 6; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; TSG-6; TSG-6tumor necrosis factor alpha-inducible protein 6; TSG6tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-stimulated gene-6 protein

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